Morphological, Phytochemical and Molecular Characterization on Some Jatropha Species Cultivated in Egypt (original) (raw)
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Genetic Relationship Analysis of Plants in the Genu S Jatropha in Thailand Using Issr Techniques
2016
There are 5 species of Jatropha in Thailand. However, Jatropha curcas is the only one which is attracted as an alternative to biodiesel. It is widely distributed in many areas in Thailand. Therefore, objectives of this study were to determine the genetic relationships among 24 samples of the genus Jatropha using Inter Simple Sequence Repeats (ISSR). A total of 39 ISSR primers were used for initial screening, only 5 primers, MAO UBC808 UBC827 UBC873 and UBC835 were found to give polymorphic patterns. These ISSR primers amplified 54 polymorphic bands (0.35-2.3 Kb). The similarity coefficient using NTSYS pc (version 2.20e) ranged from 0.66-1.00 among J. curcas and interspecific level of Jatropha ranged from 0.4720.717. The dendogram was constructed base on SHAH clustering technique of UPGMA which divided the 24 samples into 3 groups. Group I consisted of 19 samples of J. curcas and J. multifida which J. curcas (Lampoon, Thailand) was closely related to J. curcas (Laos) with high simila...
International Journal of Chemical Studies, 2019
The genetic diversity in Jatropha curcas was evaluated through RAPD marker (Random Amplified Polymorphic) in a diverse, local, panel of 25 genotypes. Out of ten RAPD primers, five showed null result, and only five were found to be polymorphic. A total of 47 marker levels were amplified that exhibited 55.39 percent polymorphism. The highest 7 bands were observed for primers OPA-02 and 6 bands for OPAW-07.The average percentage number of monomorphic bands per primer was 30 and the percentage of polymorphism ranges from 44.44(OPA-04) to 62.50(OPH09). The Jaccard`s similarity coefficient range was found to vary from 0.80 to 1.0 i.e. 80 to 100 percent. The highest value for genetic similarity (1.0) was found between Sagar (SFRI, Jabalpur) and Kaladungi rd-15, PKVJ-MKV-1 and Lower Sowan Cheack, Danibunger-28 and Low Dhearti, Danibunger-27 and Low Dhearti. The lowest value for the similarity coefficient (0.80) was found between Pant J. Sel-1 and PKVJ-MKV-1, Pant J. Sel-1and Kamaluaganja-24, Pant J. Sel-1and Lamachaur-5, Pant J. Sel-1 and Lower Sowan Cheack, Pant J. Sel-1and Lamachaur-3. The crosses among the genotypes showing lower value of genetic similarity would be more useful for plant breeding purposes rather than those genotypes having high genetic similarity. All the loci were amplified by the RAPD primer which were found to be polymorphic varied in size from 100 bp to 1500 bp. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) ordered the populations of 25 genotypes into single cluster A divided into three clusters. With cluster highest number of accession grouped under cluster 1.Lamachaur 5 showed maximum diversity with all other genotypes.it showed 15 per cent diversity.
GENETIC CHARACTERIZATION OF SEVERAL PROMISING ACCESSION OF Jatropha curcas L. BASED ON RAPD MARKER
Jurnal Penelitian Tanaman Industri, 2020
ABSTRACTThe objective of this research was to obtain genetic relationshipamong 13 Jatropha curcas L. accession plants based on Random Amplified Polymorphic DNA marker. This experiment used 13 accessionsof J. curcas L. potential to have higher seed productivity, including HS-49,SP-16, SP-38, SP-8, SM-33, SP-34, SM-35, IP-1A, IP-1M, IP-1P, IP-2A,IP-2M, and IP-2P. Polymerase Chain Reaction (PCR) was performedusing 10 selected primers of RAPD markers (OPA 2, OPA 9, OPA 13,OPA 15, OPA 18, OPA 19, OPA 20, OPF 8, OPF 10, and OPF 15). PCRproduct was used to determine genetic distance which implemented Un-weighted Pair-Group Method With Arithmetic (UPGMA) procedure andconstructed phylogeny trees using Numerical Taxonomy and MultivariateSystem (NTSYS) software version 1.8. The confidence level of UPGMAwas then tested by Boostrap using WinBoot program. Ten primers used inthis research were able to be applied in genomic DNA of J. curcas L. plantwhich had resulted about four (OPA 19) to ten band...
Molecular Biology Reports, 2009
Jatropha curcas L., a multipurpose shrub has acquired significant economic importance for its seed oil which can be converted to biodiesel, is emerging as an alternative to petro-diesel. The deoiled seed cake remains after oil extraction is toxic and cannot be used as a feed despite having best nutritional contents. No quantitative and qualitative differences were observed between toxic and non-toxic varieties of J. curcas except for phorbol esters content. Development of molecular marker will enable to differentiate non-toxic from toxic variety in a mixed population and also help in improvement of the species through marker assisted breeding programs. The present investigation was undertaken to characterize the toxic and non-toxic varieties at molecular level and to develop PCR based molecular markers for distinguishing non-toxic from toxic or vice versa. The polymorphic markers were successfully identified specific to non-toxic and toxic variety using RAPD and AFLP techniques. Totally 371 RAPD, 1,442 AFLP markers were analyzed and 56 (15.09%) RAPD, 238 (16.49%) AFLP markers were found specific to either of the varieties. Genetic similarity between non-toxic and toxic verity was found to be 0.92 by RAPD and 0.90 by AFLP fingerprinting. In the present study out of 12 microsatellite markers analyzed, seven markers were found polymorphic. Among these seven, jcms21 showed homozygous allele in the toxic variety. The study demonstrated that both RAPD and AFLP techniques were equally competitive in identifying polymorphic markers and differentiating both the varieties of J. curcas. Polymorphism of SSR markers prevailed between the varieties of J. curcas. These RAPD and AFLP identified markers will help in selective cultivation of specific variety and along with SSRs these markers can be exploited for further improvement of the species through breeding and Marker Assisted Selection (MAS).
Characterization of genetic diversity in Jatropha curcas L. germplasm using RAPD and ISSR markers
2012
Jatropha curcas L. is a rapidly emerging biofuel crop attracting a lot of interest, triggering large investments and rapid expansion of cultivation areas. In the present investigation, the genetic relationships of 29 J. curcas accessions were assessed based on randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses. A total of 72 polymorphic primers (47 RAPD and 25 ISSR) were used. Amplification of genomic DNA of the 29 genotypes, using RAPD analysis, yielded 552 fragments that could be scored, of which 334 were polymorphic with an average of 7.1 polymorphic fragments per primer. Number of amplified fragments varied from 2 to 23 and ranged in size from 100-3,500 bp. The 25 polymorphic ISSR primers used in the study produced 336 bands across 29 genotypes, of which 201 were polymorphic. The number of amplified bands varied from 7 to 20 with a size range of 100-3,500 bp. Molecular polymorphism was 60.5 and 59.8% with RAPD and ISSR markers, respectively. Mantel test between the two Jaccard's similarity matrices gave r=0.8623, showing good fit correlation between RAPD and ISSR based similarities. Clustering of genotypes within groups remained more or less similar in ISSR and combined data of RAPD and ISSR.
Research Journal of Applied Biotechnology
Calli were induced from different explants of Jatropha curcas L. seedling on MS basal agar medium in presence of different plant growth regulators. at different levels for 4 weeks Random Amplified Polymorphic DNA (RAPD) markers were used for the assessment of genetic variation of callus grown in different hormonal combinations in comparison with the mother plant. A total of 8 arbitrary sequence primers were evaluated. Of 8 primers used for the RAPD analysis, primers showed consistent band patterns. In total, scorable bands were observed with the primers. The total number of amplicons produced per primer varied from 5 for OPA-07 and OPB-12 to as many as 8 bands for OPB-10, OPC-09 and OPR-17. The average number of bands per primer was 6.625. Out of 53 bands, 36 were polymorphic (67.92%). The average number of polymorphic RAPD bands was 4.5 per primer. the highest similarity (0.71) with mother plant was recorded in leaf-derived callus (S4) grown on MS solid medium with 2.5 µM NAA plus 2.5 µM IBA, while endosperm callus maintained on MS with hormonal combination 12.5 µM IBA + BAP-25.0 µM was found to show least similarity (0.54) with mother plant. In this concern, results have been suggested to be useful fast methods for comparing of genetic changes and variation in plants.
Journal of Agricultural Science and Technology
Jatropha accessions that included 15 accessions of J.curcas and 4 different species was carried out using 3 different markers systems. Highest polymorphism (96.67%) was recorded by RAPD followed by DAMD (91.02%) and ISSR (90%). Polymorphism Information Content (PIC) was higher for DAMD (0.873) and almost equal for RAPD (0.863) and ISSR (0.862) markers, whereas Resolving Power (Rp) was found to be higher for RAPD as compared to the other two marker systems. Marker Index (MI) values varied greatly with highest (19.07) in RAPD. Shannon index (i), observed number of alleles (na), effective number of alleles (ne) and Nei's genetic diversity (h) values were found to be significantly higher for ISSR as compared to RAPD and DAMD markers. Thus, all the markers proved to be equally efficient for diversity studies in Jatropha. Several alleles in all the markers indicated J. gossypiifolia as one of the parents of J. tanjorensis. Dendrograms and PCA plots generated based on RAPD showed three major clusters with J. integerrima and J. podagrica falling in group I, fifteen J. curcas accessions in group II, and J. gossypiifolia as an outlier in group III. DAMD markers also showed similar clustering pattern whereas ISSR showed last cluster of J. gossypiifolia and J. tanjorensis. These results may provide a future base for conservation and characterization of available Jatropha genetic resources.
Molecular Biology Reports, 2012
Jatropha curcas L. (Euphorbiaceae) has acquired a great importance as a renewable source of energy with a number of environmental benefits. Very few attempts were made to understand the extent of genetic diversity of J. curcas germplasm. In the present study, efforts were made to analyze the genetic diversity among the elite germplasms of J. curcas, selected on the basis of their performance in field using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSR). The plants were selected on the basis of height, canopy circumference, number of seeds per fruit, weight of 100 seeds, seed yield in grams per plant and oil content. Out of 250 RAPD (with 26 primers), 822 AFLP (with 17 primers) and 19 SSR band classes, 141, 346 and 7 were found to be polymorphic, respectively. The percentage polymorphism among the selected germplasms using RAPD, AFLP and SSR was found to be 56.43, 57.9, and 36.84, respectively. The Jaccard's similarity coefficient was found 0.91, 0.90 and 0.91 through RAPD, AFLP and SSR marker systems, respectively. Principle component analysis (PCA) and dendrogarm analysis of genetic relationship among the germplasm using RAPD, AFLP and SSR data showed a good correlation for individual markers. The germplasm JCC-11, 12, 13, 14 and 15 whose yield found to be high were clustered together in dendrogram and PCA analysis though JCC11 is geographically distinct from others. In overall analysis JCC6 (in RAPD), JCC8 (in AFLP) and JCC 6 and JCC10 (in SSR) were found genetically diverse. Characterization of geographically distinct and genetically diverse germplasms with varied yield characters is an important step in marker assisted selection (MAS) and it can be useful for breeding programs and QTL mapping.
Genetic diversity analysis of Jatropha by Random Amplified Polymorphic DNA (RAPD)
NU. International Journal of Science, 2018
Genetic diversity analysis of Jatropha and intraspecific level of J. curcas were studied using RAPD technique. Six out of one hundred and seven primers produced polymorphic DNA patterns. A total of 144 bands were scored ranging at 0.25-3.0 kb. Genetic similarity was estimated by the Jaccard coefficient from NTSYSpc 2.20e version and ranged between 0.46-1.000. A dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) which divided Jatropha into two major clusters. The first group comprised all the 18 J. curcas accessions. J. curcas (Myanmar), J. curcas (Lumpang) and J. curcas (Chiang Mai) were grouped together with high similarity index (1.000), while J. curcas (Mukdahan) and J. curcas (India) were also clustered together. The second clade included 5 samples of Jatropha as J. gossypifolia , J. podagrica , J. multifida , J. integerrima (1) and J. integerrima (2), with the latter two clustered together and showing genetic similarity according to...