In-vitro antioxidant and GC-MS analysis ethanolic extract of poly herbal drug (original) (raw)
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In Vitro Antioxidant and Anti-Diabetic Evaluation of a Polyherbal Formulation
Journal of Natural Remedies, 2022
Diabetes Mellitus (DM) is a major metabolic disorder causing morbidity and mortality worldwide. The prominent adverse effects of allopathic drugs are the principal reason for an escalating number of people seeking alternative and complementary-based therapies that may have less or no adverse effects. The poly-herbal formulation Vilva Kudineer Choornam (VKC) is a combination of Siddha medicinal plants for the treatment of diabetes. The present study aimed to investigate the antioxidant and antidiabetic efficacy of poly-herbal formulation by invitro assays. The Aqueous Extract of Formulation (AEF) and Ethanol Extract of Formulation (EEF) were used for the assay. In the present research work, the antioxidant potential of polyherbal formulation (VKC) was evaluated using DPPH radical scavenging and Nitric oxide radical scavenging assay. The IC50 value of AEF was 333.02 ?g/ml and EEF was 86.37 ?g/ml in DPPH radical scavenging assay whereas, in nitric oxide scavenging assay, the IC50 of AE...
International Journal of Pharmacological Research, 2015
The consumption of selected plant extracts in Ayurveda, naturopathy, the antioxidant potential of the aqueous extract of Vinca rosea (VR), Gymnema sylvestre (GS), Tinospora cordifolia (TC) and Emblica officinalis (EO) and their mixture (PHF) of Indian origin was investigated by using in vitro models like superoxide, hydroxyl radical scavenging activity and lipid peroxide inhibition assay. The effects were related with standard (ascorbic acid), a known antioxidant. Various phytoconstituents identified in the above selected plants extracts were poly phenols, flavonoids, terpenoids, tannins, alkaloids. The terpenoids were stated to posess the scavenging properties against the free radicals. The presence of these phytoconstituents in selected plants might be responsible for antioxidant activity; ascorbic acid is used as a standard. Keywords: Polyherbal formulation (PHF), Anti-oxidant activity, Ascorbic acid (A.A)
Determination of Bioactive Contents and in Vitro Antioxidant Activity of Poly Herbal Formulations
INDIAN DRUGS, 2020
Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis and liver injury and cardiac complications. In polyherbal formulations HAF-I and HAF-II, described below, the total phenolics content were found to be 34.4±0.10 and 27.6±1.20 mg gallic acid equivalent (GAE)/g and total flavonoids contents, total tannin contents were 24.7±0.25 and 18.1±1.20 RE/g and 12.31±0.25 and 9.48±1.85 GAE/g, respectively. Free radical scavenging activity was determined according to the elimination of DPPH radicals. Total phenol content was determined by the Folin–Ciocalteu reaction. The relative antioxidant ability of the polyherbal formulations were investigated through two in vitro models, namely, antioxidant capacity by radical scavenging activity using α, α-diphenyl-β-picrylhydrazyl (DPPH) and nitric oxide (NO) methods. The extracts were used at 20, 40, 60, 80 and 100 μg/mL concentrations and radical sc...
Study on the in vitro antioxidant properties of selected traditional medicinal plants
Journal of Pharmacognosy and Phytochemistry, 2020
Oxidative stress is the phenomenon by which the excessive production of free radicals and oxidants that cause detrimental effects to diseases and finally death. Plants are the rich source of antioxidants which have the potential to neutralize these reactive oxygen species,thereby preserving the redox equilibrium of the body. Western Ghats serves as anexcellent habitat for many endangered species of flora and a reservoir of edible source of food for many living beings. Some of the commonly available traditional plants like Ficus benghalensis, Psidium guajava, Beta vulgaris, Syzygium cumini were known to possess rich pharmacological properties. In the present study, we aim to evaluate and compare the in vitro antioxidant properties of the selected traditional medicinal plants using various radical scavenging and reducing capacity assays. Further, the structural elucidation of the presence of phytochemical compounds in the extract was carried out using Infrared (IR) spectra. The above investigation on the free radical scavenging properties and the determination of the functional groups of bioactive compounds may open up new interest on their applications in food, pharmaceutical and cosmetic industries.
Formulation, phytochemical and antioxidant activity evaluation of selected Indian medicinal plants
Annals of Phytomedicine: An International Journal
The objective of this research was to develop a polyherbal formulation (ABA PHF) using three different herbs and to evaluate their phytochemicals, physical constants and determination of their antioxidant activity by DPPH method. The PHF authenticated herbs were characterized by studying their morphological and phytochemical analysis. Preliminary screening showed the presence of alkaloids, glycosides, carbohydrates, amino acids and flavonoids in the combination extract. Physical parameters such as loss on drying (LOD), ash values and extractive values have been studied. The antioxidant activity of the combination of extract (100 mg each) was determined using DPPH free radical scavenging method.The results showed that the combination extract has best antioxidant effect at a dose of 300 µg/ml when it was compared with ascorbic acid as the reference standard. All the extracts and PHF showed a dose dependent activity. The result showed that the PHF has more potent antioxidant activity when compared with individual extract. It also shows that the combination of three different herbs in a single formulation is completely compatible with each other which is determined from FTIR. The presence of heavy metal and minerals in the prepared extracts and PHF where analysed using atomic absorption spectroscopy. The results showed that the tested minerals and metals were within the specified limit. The result showed that the PHF has more potent antioxidant activity when compared with individual extract.
Phytochemical and In-vitro antioxidant activity of polyherbal formulation of medicinal plants
Journal of the University of Shanghai for Science and Technology, 2021
Herbal plants produce a diverse range of bioactive molecules, making them a rich source of different types of medicines. Thus, proper scientific evidence or assessment has become the criteria for the acceptance of herbal health claims. Plants-based antioxidants are now preferred to synthetic ones because of safety concerns. There is an increasing interest in natural antioxidants e.g. Polyphenols, present in medicinal and dietary plants, which might help prevent oxidative damage. Natural antioxidant increases the antioxidant capacity of plasma and reduces the risk of disease. As such developing a polyhedral formulation will definitely produce a synergistic effect as needed comparable to standard drugs that are available in the market all over the world. The polyherbal formulation, which has a combination of medicinal herbs such as Zingiber officinale (sukku), Nigella sativa (karunjeeragam), Plectranthus amboinicus (karpooravalli), Pipeer cubeba (Vaal melagu), Trigonella foenum graecu...
Evaluation Of Antioxidant Property Of Three Herbal Formulations -An In Vitro Study
SciDoc Publishers, 2021
Oxygen is an element that is invaluable to all living organisms. Although it is an indispensable part of life, it can be both beneficial as well as poisonous to the health of a body. The free radical theory of oxygen toxicity proposed by Gerschman in 1954 states that the toxicity of oxygen is due to its partially reduced forms [1]. Free radicals are chemically active atoms that have a charge due to an excess or deficient number of electrons. They can be reactive oxygen species (ROS) or reactive nitrogen species (RNS) [2]. They are cytotoxic and are considered as the etiology for various diseases
In-vitro Antioxidant Potential of a Herbal Preparation Containing Four Selected Medicinal Plants
Background: The therapeutic effects of several plants used in traditional medicine, are usually attributed to their antioxidant properties. Aim and objective: To evaluate the in-vitro antioxidant potential of herbal preparation a combination of four selected medicinal plants (HP-4) using different experimental models. Material and Methods: Polyphenols, flavonoids and flavonols concentrations and antioxidant activity of herbal preparation (HP-4) as compared to butylated hydroxyl toluene (BHT) and átocopherol in various experimental models were evaluated. Results: The antioxidant activities of HP-4 were concentration dependent in different experimental models and were comparable to activities of BHT and αtocopherol. Conclusion: Polyherbal formulation of HP-4 is better than individual plant extracts.
Majalah Obat Tradisional, 2022
Chinaberry (Melia azedarach L.) leaves, Malayan cherry (Muntingia calabura L.) fruits, and Yacon (Smallanthus sonchifolius (Poepp.) H.Rob.) leaves are used for traditional diabetes treatment by Banyumas (Central Java, Indonesia) people. This study characterized selected quality parameters, evaluated the invitro antioxidant activity as the preliminary assay for its antidiabetic activity, and calculated the total flavonoid content (TFC) and total phenolic content (TPC) of those crude drugs. The plant materials of each species were collected from three different areas in Banyumas and dried into crude drugs. The quality parameters were determined according to the standard method in the Indonesian Herbal Pharmacopeia (IHP) 2017. The antioxidant activity was evaluated by the standard 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing antioxidant power (FRAP) assays, while TFC and TPC were analyzed following standard methods in IHP 2017. The correlations between antioxidant activities and the TFC-TPC were analyzed by Pearson's correlation test. The values for acid-insoluble ash, loss on drying, total ash, ethanol extractable, and water-extractable of all crude drugs were quantified, with TPC and TFC proposed as the chemical content parameter for Malayan cherry fruits and Yacon leaves. Yacon leaves and Malayan cherry fruits contained the highest TFC and TPC at 8.01±0.72 mg Quercetin equivalent (QE)/g dry weight (DW) and 11.54±1.44 mg Gallic acid equivalent (GAE)/g DW, respectively. Malayan cherry fruits exerted the strongest DPPH scavenging activity (366.13±17.65 mM Trolox equivalent (TE)/g DW) and FRAP (1025.33±50.47 mM TE/g DW). Moderate to strong correlations were observed between DPPH radical scavenging activity-FRAP and TFC-TPC of Malayan cherry fruits and Yacon leaves. Hence, flavonoids and phenolic compounds of both crude drugs contributed to their antioxidant activity.
2022
Oxidative stress and free radicals-induced diseases can be managed by introducing clinically proven naturally occurring antioxidants or antioxidant supplements which can prevent the onset of many cardiovascular diseases, neurodegenerative disorders, and cancers. Collection, identification, and preservation of plant material, sample preparation from plant sources using suitable solvents. Rapid screening of plant extracts for antioxidant activity and Electron Transfer methods (ET) assay. In-vitro Antioxidant Methods Hydrogen Atom Transfer methods (HAT). These antioxidants have a strong potential in the conventional treatments of diseases especially in inflammatory disease, neurodegenerative diseases, cancer, and diabetes. Hence, with a balanced diet and best supplementation of fruits, vegetables, grains, oils, and nuts having adequate essential antioxidants such as vitamin A, E, C, lipoic acid, etc., are sufficient to improve our body’s immune system.