Spectral Manifestations of Thioflavin T Aggregation (original) (raw)

Journal of Applied Spectroscopy, 2015

Abstract

ABSTRACT Absorption, fluorescence, and fluorescence excitation spectra of thioflavin T in aqueous solutions and polyvinyl alcohol films were studied. The dye aggregated when its concentration was increased. This was accompanied by a hypsochromic shift of the absorption spectrum and a bathochromic shift of the fluorescence spectrum. It was shown that fluorescence of the probe embedded in amyloid fibrils was caused by monomers rather than dimers or other aggregates.

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