THE GROWTH PERFORMANCE AND YIELDS OF STEVIA (Stevia Rebaudiana BERTONI) RESPONSES TO DIFFERENT TYPES OF FERTILIZER (original) (raw)

Growth Response of Five Cultivars Stevia (Stevia rebaudiana Bertoni) Using Commercial Fertilizers as Basic Media In Vitro

2021

Background: Conventional propagation of seedling stevia is limited by parent material. Propagation by seeds that are difficult due to low of germination rate. Propagation through tissue culture can be applied as an alternative for propagation stevia and can produce last of seedling and in short-time in suitable media. Generaly, culture media is quite expensive, so it is necessary to find an alternative types of culture media. This study aimed to evaluate the growth response of five stevia cultivars on culture media containing commercial fertilizers. Materials and Methods: A Randomized Block Design (RBD) was used in this experiment with two factors and replicated three times. The first factor consisted of five culture media DKW, MS, AB Mix, Grandasil, and Growmore. The second factor consisted of Stevia cultivars i.e. Kanada, Bogor, Garut, Tawangmangu, and Pengalengan. Results and Conclusion: The result showed that the best responses to the character number of shoots (28.9 shoots), sh...

Yield, Nitrate and Steviol Content of Stevia (Stevia Rebaudiana Bertoni) Leaves as Affected by Fertilization Rate

Acta horticulturae, 2012

Stevia (Stevia rebaudiana Bertoni) is cultivated for the sweet taste of its leaves, which are, after drying and grinding used as a substitute for artificial sweeteners. The aim of this study was to determine the effect of fertilizer dose on the fresh leaves yield, nitrate and steviol content. Plants were grown in 5-L pots on the two types of acid soil (pH 4.0 and 5.0), which were supplied with different major nutrients. Five doses (0 to 1.6 g per plant) of mineral fertilizer Multi-Comp Base 14:13:20 (N:P:K)+2MgO+microelements were applied. The highest dry leaves yield (30.28 and 30.98 g per plant) was achieved by applying 1.6 and 0.8 g of fertilizer on soil with pH 4.0 and 5.0, respectively. These yields were 21.6, and 39.8% higher than the yield achieved under unfertilized control. Increased fertilization intensity resulted in higher amounts of total nitrogen in stevia leaves. Depending on the soil type, amounts between 14.32 and 20.61 g per kg of dry matter (DM) were obtained. At the soil with pH 4.0, higher fertilization rates decreased the amount of N-NO 3-in leaves from 18.16 to 10.05 mg per kg DM. At pH 5.0, only the fertilization with 1.6 g per plant increased the amount of nitrate in leaves compared to the unfertilized control (14.14 compared to 13.69 mg per kg DM). The amount of N-NH 4 + in the leaves showed the opposite trend. Increased fertilization rates resulted with an increased accumulation of ammonia in leaves, passing from 214.55 to 387.83 mg per kg DM. Average glycosides content was between 42.0 and 45.7 mg SE (steviol equivalents) per g of dry leaves. Stevia cultivation on the soil with higher pH value and medium fertilization rate resulted in economically profitable yield of leaves which contained significant steviol amount.

Effect of Environmentally Friendly Nutrition Supply on Stevia (Stevia rebaudiana B.) Production

Notulae Botanicae Horti Agrobotanici Cluj-napoca, 2018

The experiment was carried out on raised bed (3 rows) with 33 cm row distance and 25 cm between the plants and 24 plant m-2 density. The plantation was prepared on 28 May, 2015 with about 10 cm high transplants. The soil surface was mulched with wooden chips (5 cm layer). The aim was to evaluate the environmentally friendly fertilizers (Dudarit-150 g m-2 , Sprintalga-0.04% concentration four times 200 mL per plant). During the vegetation period pruning was applied at 30 cm plant height at the 4 th double leaf from the soil surface and two cuttings (13 Aug and 29 Sept). By evaluating the effect of treatment on the leaf surface and chlorophyll content, statistically significant results were not found. Higher SPAD (relative chlorophyll content) value was measured in the middle of the growing period compared to the end of the vegetation when the plants were older and the temperature started to decrease. The positive effect of Dudarit and Sprintalga was clear on the plant height and biomass production. The dried herb yield was higher (4 kg m-2) on treated plots comparing to control (3 kg m-2). Although increased photosynthetic intensity was not detected on treated plots, the plantation showed greater vitality and side shoot improvement to control plots. Under our circumstances, the environmentally friendly fertilization (Dudarit and Sprintalga) has increased the dried leaves yield with 20% by similar stevioside and rebaudioside-A content.

Influence of Plant Population Density on Growth and Yield of Stevia (Stevia Rebaudiana Bertoni L.)

International journal of Advanced Biological and Biomedical Research, 2017

Objective: An experiment was conducted at Wondo Genet Agricultural Research Center in the production season of 2013/14 and 2014/15 with the objective of identifying the best combination of intra and inter-row spacing for optimum plant population density of stevia. Methods: The experiment was conducted using five intra-row spacing (20cm, 25cm, 30cm, 35cm, and 40cm) and three inter-row spacing (40cm, 50cm and 60cm) with total treatment combination of fifteen that were laid out in factorial RCBD design with three replications. In 2013/14 cropping season the maximum fresh leaf weight (19467kg ha-1), fresh above-ground biomass (25002kg ha-1) and dry leaf weigh (7834kg ha-1) were obtained from the combined pacing of 20cm intra-row and 40cm inter-row spacing. In 2014/15 cropping season, the maximum fresh leaf weight (16470.1 kg ha-1) and (14433.9kg ha-1), fresh above-ground biomass (27547kg ha-1) and (23619.8kg ha-1) and dry leaf weight (4773.7 kg ha-1) and (4314.0 kg ha-1) were obtained...

Response of Stevia (Stevia rebaudiana Bertoni M) To Nitrogen and Potassium Fertilization

Abstract: Objective of this research was to obtain optimal dosages of nitrogen and potassium fertilization on growth, yield, and quality of stevia. The research was conducted using Randomized Block Design (RBD) by 3 replications, which was constructed in factorial that comprised of 2 factors. The first factor was dosage of the nitrogen with 4 (four) levels, such as: 100, 150, 200, and 250 kg N ha-1. The second factor was dosage of potassium with 3 (three) levels, such as: 75, 150, and 225 kg K2O ha-1. Result of the research showed that significant interaction between nitrogen and potassium fertilization has occurred on parameter of the plant growth, such as number of leaf, leaf area, leaf area index, and the total chlorophyll content. Separate application of nitrogen and potassium fertilization has significantly affected on the plant height, while the stomatal density was only affected by the application of nitrogen. Significant interaction between nitrogen and potassium application has occurred on plant biomass and the yield of leaf during the harvest period I, II, and total. During the period of total harvest, the fertilizing treatments of 200 kg N ha-1 and 225 kg K2O ha-1 have harvested fresh leaves and dry leaves for about 2,780 kg ha-1 and 636 kg ha-1, respectively. The increasing application of nitrogen from 100 to 250 kg N ha-1 has significantly increased the stevioside level of the stevia’s leaf linearly in accordance with the equation of Y = 0.000408 X + 0.439. Keywords: Stevia, nitrogen, potassium, fertilization, growth, yield

Influence of bio-fertilizers on the biomass yield and nutrient content in Stevia rebaudiana Bert. grown in Indian subtropics

A pot culture experiment was conducted at Indian Institute of Horticultural Research, Hessaraghata, Bangalore, India to study the effect of bio-fertilizers on the biomass yield and NPK content in Stevia (Stevia rebaudiana). The results show the yield and NPK content in stevia plant has been found to be increased initially and thereafter, the amount of the same decreased with the progress of plant growth up to 60 days with the combined treatment of bio-fertilizers rather than individual treatment. This is due to their ability to fix atmospheric nitrogen (symbiotic and asymbiotic) and transform native soil nutrients likely phosphorus, zinc, copper, iron, sulfur from the non-usable (fixed) to usable form and decompose organic wastes through biological processes which in turn releases nutrients in a form which can be easily assimilated by plants resulting in an increase in biomass production of stevia plant.

Effect of Mineral Nitrogen Fertilization and Compost on Stevia Yield and its Profitability

Journal of Soil Sciences and Agricultural Engineering, 2017

Stevia (Stevia rebaudiana Bertoni) was recently introduced to Egyptian agriculture in order to produce a natural sweetener (Steviol glycosides) instead of sugar (Sucrose) to cover some of the lack of sugar production, which reached annually about 0.8 million ton. So, a field experiment was carried out at Mallawi Agriculture Research Station , Minia Governorate , Egypt, during two successive seasons (2014 and 2015) to study the effect of different levels of mineral N fertilization and compost on yield and its quality of stevia (Stevia rebaudiana Bertoni) under Middle Egypt conditions. The experimental design was a split plot in three replicates and the main plot were devoted N fertilizer levels (40, 60 and 80 kg N fed-1) while compost levels (0, 3 and 6 ton fed-1) were allocated in the sub plot. The obtained results revealed that N fertilizer level and/or compost level had a significant effect on plant height (cm) , fresh and dry leaves weight (g plant-1) , N, P& K contents of stevia leaves and N uptake kg fed-1 , dry leaves yield kg fed-1 , stevioside (St %), rebaudioside A (Rb%), stevioside yield (kg/fed) and rebaudioside A yield (kg fed-1), the remained nutrients (N,P & K (ppm) and OM %) in the soil post-harvest of stevia in two growing seasons, except P% of leaf in the 2 nd season for N fertilization. A significant interaction was scored between N fertilizer level and compost rate with regard to plant height (cm) , fresh and dry leaves weight (g plant-1) , N % of stevia leaves and N uptake kg fed-1 , dry leaves yield kg fed-1 , stevioside (St %), rebaudioside A (Rb%), stevioside yield (kg fed-1) and rebaudioside A yield (kg fed-1) of stevia in the two growing seasons. From the present study , it may be concluded that the application of 60 kg mineral N fertilizer + 6.0 ton compost fed-1 was the best treatment for improving the yield and quality of stevia and is advisable to achieve the highest values of rebaudioside A (298.99 and 297.74 kg fed-1) ,and net profit of stevia.

The Effects of Plant Density and Plant Age on Yield of Stevia (Stevia rebaudiana Bertoni) in Çukurova Conditions

Journal of the Institute of Science and Technology, 2017

An experiment was conducted at Wondo Genet Agricultural Research Center in the production season of 2013/14 and 2014/15 with the objective of identifying the best combination of intra and inter-row spacing for optimum plant population density of stevia. Methods: The experiment was conducted using five intra-row spacing (20cm, 25cm, 30cm, 35cm, and 40cm) and three inter-row spacing (40cm, 50cm and 60cm) with total treatment combination of fifteen that were laid out in factorial RCBD design with three replications. In 2013/14 cropping season the maximum fresh leaf weight (19467kg ha-1), fresh above-ground biomass (25002kg ha-1) and dry leaf weigh (7834kg ha-1) were obtained from the combined pacing of 20cm intra-row and 40cm inter-row spacing. In 2014/15 cropping season, the maximum fresh leaf weight (16470.1 kg ha-1) and (14433.9kg ha-1), fresh above-ground biomass (27547kg ha-1) and (23619.8kg ha-1) and dry leaf weight (4773.7 kg ha-1) and (4314.0 kg ha-1) were obtained from 20cm intra-row and 40cm inter-row spacing respectively. Results: Although the study showed that the highest Stevia herbage yield per unit area was recorded from the combined spacing of 20cm intra-row and 40cm inter-row spacing, considering the difficult condition we met during weeding and watering, we, therefore; suggest that the best combined intra-row and inter-row spacing for Stevia is 25cm x 40cm to attain maximum yield under appropriate management conditions at wondo genet and similar locations.

Evaluation of stevia (Stevia rebaudiana Bertoni.) Genotypes performance on growth and chlorophyll accumulation

2019

The present investigation was carried out in 2018-19 to evaluate the performance of nine stevia genotypes viz., CIM Madhu, CIM Mithi, Kodaikanal Local, Pune Local, Zaheerabad Local, GKVK Stevia-1, Indore Local, Hyderabad Local and Bidar Local for their vegetative growth and chlorophyll content under the Northern Dry Zone of Karnataka. The experiment was laid out in Randomised Complete Block Design (RCBD) with three replications at the Main Horticultural Research and Extension Centre (MHREC), Bagalkot, Karnataka. The parameters like plant height, number of leaves per plant and chlorophyll reading were recorded at different growth stages. The maximum plant height (46.02 cm), number of branches per plant (57.60), number of leaves per plant (895.43), plant spread in East-West (31.47 cm) North-South (31.23 cm) directions and maximum chlorophyll reading (53.67) at harvest were recorded for the stevia genotype, GKVK Stevia-1.

Stevia (Stevia rebaudiana Bertoni ) responds to different levels of nitrogen and potassium fertilizers in loamy sand soil

Muthanna Journal for Agricultural Sciences, 2021

Re ce ived 2021 / 1 / 15 Acce pted date 2021 / 2 /17 A study was conducted in Al-Seba Reserve / Basra Governorate to study the effect of nitrogen and potassium fertilization on growth of stevia plants during 2018-2019 agricultural season, in pots. A sandy mixture of soil was used and two factors were studied: the first factor was urea fertilizer with five levels of nitrogen (N 0 0, N 1 100, N 2 150, N 3 200, and N 4 250 kg / ha) and the second factor was potassium sulfate with three levels of potassium (K 0 0, K 1 75 and K 3 150 kg /h (. The experiment was experimental factor using a complete randomized design (C.R.D) with three replications. The results showed a significant effect of adding nitrogen and potassium fertilizers and there interaction on: plant height, number of branches, leaf area index, and the treatment N 3 K 2 gave the highest yield reached (1.27 tons. h-1), and N 4 K 2 recorded a highest content of Rebaudioside A (53.26 ppm).