Genetic Variation Analysis of Sinai Chicken and Japanese Quail Populations Using Microsatellite DNA Markers (original) (raw)
Tow avian species; Japanese quail and Sinai chickens were examined genetically using 3 microsatellite markers to detect genetic variation. The studied loci on average produced 5.666 alleles per locus (range: 4-8). The mean observed heterozygosity (H o ) was 0.568 and ranged across loci from 0.125 to 0.900, whereas the mean expected heterozygosity (H e ) was 0.697 and ranged between 0.611 and 0.843. The polymorphic information content (PIC) values varied among loci and ranged between 0.519 for locus GUJ0063 and 0.806 for locus GUJ0087 with overall mean 0.637. Differentiation among populations was high (F ST = 0.311; R ST = -0.042). Sinai chickens showed no departure from Hardy-Weinberg equilibrium, while Japanese quails were not in Hardy-Weinberg equilibrium. These results reflect that, the set of studied markers can be used effectively to capture the magnitude of genetic variability in both of Sinai chicken and Japanese quail populations.
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