Effects of glucose concentration on the sperm motility of the catfish Sorubim cuspicaudus (original) (raw)

The aim of the study was to evaluate the effect of glucose concentration (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10%) in the activation of sperm motility of the catfish Sorubim cuspicaudus. Semen was obtained by hormonal induction, with salmonid GnRH analogue (10 mg/Kg body weight) of males undergoing spermiation (n = 13). The osmolarity of the extender and the seminal plasma (273.28±7.2 mOsm/Kg) was measured with an osmometer (Osmomette III, Precision Systems, USA). The semen and extender were mixed in proportion 1:3. The total motility and activation time were evaluated in the glucose solution and post-dilution, activated with 75 µL of distilled water in a Makler chamber with the assistance of software Sperm Class Analyzer (SCA, Microptic, Spain).