PIX5 Evaluation of diagnostic methods of cytomegalovirus infection in Egyptian renal transplant recipients (original) (raw)

was consistent with acute graft-versus-host disease (GVHD). A second attack happened on posttransplant day 80. CSF, blood, urine analysis and imaging studies were not remarkable for bacteria, CMV, EBV, HSV, toxoplasma, fungi, leukemic relapse and other neoplastic cells. In the posttransplant period, erythrocyte and platelet concentrates were transfused. It was suspected that the causing agent might be West Nile virus. Then, WNV was investigated by a new developed TaqMan based one-step real time reverse transcriptase polymerase chain reaction (RT-PCR) assay for detection and quantification of WNV RNA with oligos targeting 3 non coding region. The first WNV was detected in a blood sample of a patient with acute GVHD in our hospital. Since Turkey is in the Mediterranean Basin where WN fever is an endemic disease, it is critical to investigate WNV in risk groups.