Performance of Serological Diagnosis of TORCH Agents in Aborted versus non aborted Women of Waset province in Iraq (original) (raw)
Related papers
2017
Recurrent Abortion (RA) in the general populations is common occurrence and often changes among different communities. This study was to determination the prevalence of Rubella, Cytomegalovirus, herpes, and Toxoplasma Gondii in Baghdad. Blood samples were collected from 210 women, 180 women with recurrent abortion (three or more abortions) in the first trimester and 30 women with a healthy pregnancy. Based on the clinical examination, and diagnostic laboratory findings of the rapid lateral immune chromatography assay (RLICA), and Enzyme-linked Immunosorbent assay (ELISA) test for TORCH examination measured serum levels of immunoglobulin (IgM and IgG) for Toxoplasmosis, cytomegalovirus, herpes, and rubella in aborted women. Depending on the result of ELISA test the patients were included women with recurrent abortion with sero-negative for TORCH test was 50 (27.8%), women with recurrent aborted with sero-positive for antitoxoplasma antibodies was 44 (24.5%), women with recurrent abor...
Serodiagnosis of Torch Infections among Aborted Women in Duhok City/ Kurdistan Region /iraq
The Journal of The University of Duhok
TORCH syndrome is a cluster of symptoms caused by congenital infection with (Toxoplasma gondii, Rubella virus, Cytomegalovirus, Herpes simplex virus, and other pathogens such as Hepatitis B virus antigen and syphilis) during the gestation period of pregnancy. The infection might lead to fetal abnormalities or abortion. Objective: The current study was done to detect TORCH pathogens, hepatitis B virus, and syphilis antigens in women with a previous history of recurrent abortion using serological test in Duhok city. Materials and method: A direct rapid test was used as a serological method for the detection of IgM and IgG antibodies against TORCH pathogens as well as the seropositivity of HBV antigen and syphilis. A total of 90 pregnant women with bad obstetric history were included in the current study throughout April-August 2017 in Duhok City/ Kurdistan Region/Iraq. Results: The total seropositivity of anti-pathogens (Toxoplasma gondii, Rubella virus, Cytomegalovirus, and Herpes virus) were 19/90(21.1%), 17/90(18.9), 16/90(17.8), 16/90(17.8%) and for HBs Ag and syphilis 3/90 (3.3%) and syphilis 1/90(1.1%) respectively. The IgM and IgG seropositivity to Toxoplasma gondii, Rubella virus, Cytomegalovirus, and Herpes viruses were (13.3% and 7.8%), (10.0% and 8.9%), (10.0% and 7.8%), and (10.0% and 7.8%) respectively. Seroprevalence of antipathogens IgG and IgM antibodies associated with socioeconomic and demographic characteristics were indicated in the results section. Conclusion: A previous history of pregnancy wastage and the serological test for TORCH infection in addition to HBs Ag and syphilis during current pregnancy must be considered while managing BOH cases as to reduce the adverse fetal outcomes, the early diagnosis of TORCH using serological methods is highly significant.
Incidence of Miscarriage in Pregnant Women due to TORCH Co-Infection in Erbil City/ Iraq
Journal of University of Babylon for Pure and Applied Sciences, 2021
Background: TORCH infections in pregnant women can result in serious effects, such as miscarriages, stillbirths, intrauterine growth retardations and congenital anomalies. TORCH infections caused by Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus, and these organisms can transmit from mother to fetus through the placenta. The study aimed to investigate TORCH infection in pregnant women. Materials and Methods: A cross-sectional and analytical study was conducted on 268 pregnant women (21-45 years) with miscarriage history. Blood samples were taken from all participants for screening TORCH infection. The screening was performed via Cobas e411 and using ROCH test kits manufactured by ROCH Company, Germany. Results: Toxoplasma gondii was found in the highest percentage of TORCH infection (45.1%), followed by Cytomegalovirus CMV (21.6%), then Rubella (18.3%), which was found in the lowest percentage of infection. Non-significant differences were observed among age groups concerning Toxoplasmosis and Rubellosis, but significant differences were observed with CMV infection. Toxoplasma infections were observed with a high rate of infection (61.2%) in house wife, but there were no significant differences in Rubella and CMV infection based on occupation. The highest rate of toxoplasmosis (63.6%) was recorded in the first trimester of gestational age, with a significant difference, whereas there were no significant differences between gestational age with Rubella and CMV infections. Correlation was recorded between toxoplasmosis and the number of miscarriages with the highest rate (68.9%) in the number of miscarriages, whereas a non-significant correlation was recorded between Rubella or CMV infection and the number of miscarriages. Conclusions: In TORCH infection, Toxoplasmosis are observed in high rate (45.1%) among pregnant women suffered from previous miscarriages, and significant correlation between toxoplasmosis and the number of miscarriages was found but this correlation was non-significant with Rubella and CMV infection.
Abstract In this work 180 blood samples was collected from pregnant women in Babylon province, Babylon maternity and children hospital from October/2008 to April/2009. It revealed that TORCH infections was; Cytomegalovirus formed (CMV) 57.2% followed by Toxoplasma gondii 55.5% Rubella 53.9%, Herpes simplex II 28.9% and Chlamydia trachomatis 24.4%. Seroprevalence of Toxoplasma, Rubella, CMV, Chlamydia trachomatis and Herpes IgM Antibodies according to various obstetric losses showed that Abortions happened in all causes with high percentage (Over than 30%) except Herpes infections (less than 6%), while congenital anomalies and premature delivery formed high ratio with some different in some cases. Neonatal deaths are very low under 1% except in CMV infections which formed 4.9%. Distribution of age with type of infection according IgM Antibodies to Toxoplasma, Rubella, CMV, Chlamydia trachomatis and Herpes simplex revealed that major age group for infection was between <20 to 40 years which formed more than two third of all infection cases. Residential distribution with type of infection according IgM Antibodies shows that most infection occurred in rural area (over than 50% in all agents) except in Herpes simplex infections which formed 82.7% in urban area. TORCH (Toxoplasma gondii, Rubella, Cytomegalovirus and Herpes simplex) infections with incidence of abortion in pregnant women in this study revealed that First trimester was the highest ratio of infection than other two trimesters.
Seroprevalence of TORCH in women with spontaneous abortion and stillbirth, in Asmara, Eritrea
Population Medicine
INTRODUCTION Toxoplasma gondii, others, rubella, cytomegalovirus and herpes simplex (all together denoted as TORCH) are the major causes of bad obstetric history (BOH) and may cause birth defects. Therefore, antenatal screening for antibodies to TORCH infectious agents is an important tool for diagnosis. Thus, the study was conducted to determine the seroprevalence of TORCH among mothers with a loss of pregnancy (abortion) or stillbirth. METHODS A laboratory-based cross-sectional study on the frequency and associated risk factors for the prevalence of TORCH was conducted from 28 February to 31 March 2018. The study included 44 women aged 18-45 years who were admitted at Orotta Maternity National Referral and Teaching Hospital, Asmara, Eritrea, due to loss of pregnancy (abortion) or stillbirth. The study participants were selected by systematic random sampling. From each woman, 3 mL of venous blood was collected in a container with aseptic technique. Immunoglobulin G antibodies against Toxoplasma gondii, rubella, cytomegalovirus and herpes simplex were detected by IgG Combo Rapid Test Cassette (Serum). RESULTS Of the 44 mothers with mean age of 29.3 years (SD=5.6), who were tested by IgG Combo Rapid Test Cassette
Association of TORCH Agents with Spontaneous Abortion in Patients with Bad Obstetric History
The aim of this study was to evaluate the associations between prevalence of TORCH (Toxoplasma gondii, Rubella virus, Cytomegalovirus/CMV, Herpes simplex virus) infection and abortion in pregnant women. In the present work, the prevalence of IgM and IgG antibodies to TORCH agents were investigated in women with Bad Obstetric History (BOH) using ARCHITECT IgM/IgG assay. A total of 55 serum samples were collected from pregnant women having BOH. Eleven (20%) were positive for Toxoplasma, 55 (100%) for Rubella, 55 (100%) for CMV alone or in combination. There was no IgM, or IgG antibodies to Herpes simplex virus found in all samples. The present study revealed co-infection of Rubella and CMV and mixed infection with Toxoplasma. IgM antibodies were not detected against Toxoplasma and CMV but 6 sera (10.90%) were positive for IgM antibodies against Rubella. It is concluded that antibody positivity was highest for Rubella and CMV followed by combination of Toxoplasma and Rubella and CMV and Rubella respectively. The study shows that there is a strong association of these agents with BOH. Thus, screening and early diagnosis for these pathogens in women can help in proper management of these cases.
2016
Viral and bacterial infections in pregnant women have long been known to play a critical role in pregnancy wastage and adverse fetal consequences in terms of recurrent and spontaneous abortions especially at the first trimester of gestation with the increasing risk of in-utero transmission. Aim of the study: The correlate between the incidence of Rubella, CMV, HSV and Ct primary infection among first trimester gravidas with recurrent abortion, and estimating the rate of mixed infection with these agents. Materials and methods: A cross-sectional study was carried out at the private laboratories of Wasit province/Iraq between September 2015-August 2016 serum level of Rubella, Cytomegalovirus (CMV), Herpes simplex virus 1and 2 (HSV) and Chlamydia trachomatis(Ct) antibodies were evaluated using enzyme-linked immunosorbent assay (ELISA) method and IgG avidity. Results: 82 serum samples were collected from gravidas in their first trimester, the study group included 67 (81.7%) gravidas suf...
Saudi Medical Journal, 2020
Objectives: To investigate the presence of toxoplasmosis, rubella, cytomegalovirus, and herpes (TORCH) infections in women attending at the antenatal care clinic in Abha, Kingdom of Saudi Arabia (KSA). Methods: A total of 190 blood samples were collected from Abha maternity hospital in Aseer region, KSA, from February 2018 to May 2019 and screened with the TORCH panel (toxoplasmagondii [IgG/IgM], cytomegalovirus [CMV] [IgG/IgM], rubella [IgG/ IgM], and herpes simplex type 1 and 2 [IgG/IgM]). Results: The mean age was 31.42±6.514 years and gestational age was 32.48±6.168 weeks. Serum IgG was positive for Toxoplasma gondii (T. gondii) (27.4%), herpes simplex type 1 (HSV-1) (94.7%), herpes simplex type 2 (HSV-2) (0.5%), CMV (100%), and rubella (88.9%). Serum IgM was positive only for CMV (9.5%). Though, there was an association between abortions from previous pregnancies (26.5%), intrauterine death (5.8%), premature labor (3.2%), microcephaly (1.6%), other congenital diseases (1.6%) and low birth weight (0.5%) with current IgG positivity for TORCH infections, the results were not statistically significant. Conclusion: Seropositivity for IgG antibodies correlate with TORCH-associated pregnancy complications in Abha, KSA; however, IgM positive CMV pregnant cases warrant further systematic investigation to understand the implications of CMV on outcomes during pregnancy.