Journal of Veterinary Medicine and Research Evaluation of the Antischistosomal Effect of Poly I:C or Zymosan against Schistosoma Mansoni Infectious Disease (original) (raw)

Introduction: In this study we focused on novel antischistosomal toll-like receptor (TLR) agonists which induced immune cells to produce a plethora of inflammatory cytokines against shistosoma mansoni infection. We used in this study two importants agonists TLR3L (polyinosinicsalsidylic acid; poly I:C) andTLR2L zymosan. We divided this study into 2 experiments 1. The adjuvant effect of polyI:C orzymosan with soluble worm antigen (SWAP antigen) and we divided it into negative control group, positive control group,vaccinated group with SWAP antigen, vaccinated group with SWAP antigen plus poly I:C,vaccinated group with SWAP antigen pluszymosan 2. Antischistosomal effect of poly I:C orzymosan and we divided it intonegative control group, two positive control groups, treated groups with poly(I:C) on day 25 or day 35 after infection, treated groups with zymosan on day 25 and day 35 after infection. Antischistosomal effect of poly I:C or zymosan was evaluated by measuring serum biochemical parameters as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, albumin and total protein. We measured also oxidative stress parameters such as glutathione S. transferase, catalase, total antioxidant capacity, total thiol and malondialdehyde in liver homogenate, in addition to the histopathological examinations of all mice liver tissue with measuring granuloma sizes of all infected and treated groups and finally we measured the parasitological parameters as worm burden, liver and intestine eggs count.

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