African Journal of Microbiology Research Antibacterial susceptibility spectrum of some gram negative bacteria from suspected Otitis media patients (original) (raw)
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African Journal of Microbiology Research, 2016
Otitis media (OM) is a multifactorial disease characterized by high rate of recurrence in young children and considered to cause hearing impairment in children. This study was undertaken to evaluate the antibacterial sensitivity of Gram-negative bacteria of OM to some antibiotics. A total of Fifty four (54) samples were obtained from patients with suspected cases of otitis media coming into the National Ear Care Centre for the first time. Fifty four (54) patients (29 male, 25 female), with mean ages of 3.86 and 3.29 respectively, tested positive to OM pathogens with a total of 84 bacterial isolates while no culturable pathogen was observed in 4 patients (7.4%). The study reveals highest frequency of Pseudomonas aeruginosa 60 (71.43 %) followed by Proteus mirabilis 14 (16.67), Klebsiella pnuemoniae 6 (7.14) and Escherichia coli 4 (4.76). P. aeruginosa had the highest prevalence among patients < 30 years, whereas K. pnuemoniae and E. coli had the least isolated across all age group...
Antibiotics susceptibility of bacterial pathogens associated with otitis media
African Journal of Bacteriology Research, 2010
One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65(54.2%) males and 55(45.8%) Females, with male: females ratio (1.2:1) and 60 individual without ontological problems as control group were included in this study, which was done during the period of March, 2009 to January, 2010. This Include collection of aural swab samples, culturing of samples, identification of causative agents species and antibiotic sensitivity. Gram ' s negative bacteria were the commonest microorganisms; it comprises (60%). Pseudomonas aeruginosa was common causative agent (19.04%), followed by Staphylococcus aureus (16.7%) and Klebsiella spp. (14.3%). Mixed infection was found in high percent (74%), in which P. aeruginosa and other microorganisms were more common. The antibiotic sensitivity pattern showed that P. aeruginosa was sensitive to Ciprofloxacin, amoxicillin +clavulanic acid and gentamicin, while other is appeared resistant, S. aureus was sensitive to ciprofloxacin, amoxicillin+clavulanic acid, erthomycin, cephalexine and it is resistant to pencillin and ampicillin, klebsiella species were sensitive to ciprofloxacin, amoxicillin +clavulanic acid ,gentamicin,while resistant to tetracycline.
Bacteriology and Antibiograms of Chronic Otitis Media Among Children in Hyderabad
Journal of Evolution of Medical and Dental Sciences, 2016
BACKGROUND Suppurative otitis media is a very common disease in ENT practice in all age groups, but in children it represents an important cause of preventable hearing loss. Physicians attending such patients tend to use antibiotics in the first instance empirically. Even though many studies are available on the susceptibility profiles of the bacteria isolated from the ear discharge, the susceptibility of these organisms varies from time to time and from place to place. Hence, such studies are required periodically to evaluate the changing trends in bacterial susceptibility following isolation. Objective of this study is to isolate and identify the bacteria and study their antibiotic susceptibility profiles in children attending the Government ENT Hospital in Hyderabad. MATERIALS AND METHODS Between December 2008 and November 2012, a cross-sectional study was conducted at Government ENT Hospital, Koti, Hyderabad on 320 ear discharge samples collected from children attending with complaints of suppurative otitis media. Standard microbiological procedures adopted in collection, isolation and culture sensitivity of the samples. RESULTS Bacterial isolates encountered were Staphylococcus aureus (42.46%), E. coli (22.14%), Pseudomonas aeruginosa (16.02%), Proteus species (11.76%) and Streptococcus species (7.62%). The gram positive bacteria showed higher sensitivity to Ceftriaxone sodium (~ 90-100%), Cefotaxime (~ 90%) and Cefaclor (~ 85-90%). The Gram negative bacteria showed sensitivity to Amikacin (~ 100%), Gentamicin (~ 90-100%) and Ceftriaxone sodium (~ 80-95%). Pseudomonas aeruginosa showed resistance to many of the antibiotics used for testing sensitivity and termed as MDR (Multidrug Resistant Bacteria). CONCLUSION Among the clinical isolates, low levels of resistance was found in general to the commonly used antibiotics in the community. An increased frequency of (Methicillin-Resistant Staph aureus) MRSA was found. Awareness among the public and treating physicians should be created to reduce the risks of developing complications of suppurative otitis media and empirical treatment protocols that should be followed.
Pattern of bacterial pathogens of acute Otitis media in a tertiary hospital, South Western Nigeria
African Journal of Clinical and Experimental Microbiology, 2016
INTRODUCTION: Otitis media was reasonably prevalent prior to the use of antibiotics for treatment. In Nigeria, hospital incidence reports indicate that chronic suppurative Otitis media is the commonest. Complications that usually arise as a result of untreated Otitis media are meningitis, brain abscess, keratoma, otosclerosis, and hearing loss. The study aimed at providing information on the pattern of bacterial pathogens of acute Otitis media in LAUTECH Teaching hospital, Osogbo, Nigeria. METHODS: It was a cross-sectional study involving patients with acute Otitis media attending ENT clinic at LAUTECH Teaching Hospital, Osogbo, Nigeria. Ear swabs were collected from the patients after informed consent. The samples were inoculated on general and selective laboratory media. Bacterial pathogens were isolated and identified. Antibiotic susceptibility testing was performed on each of the bacterial isolates using modified Kirby Bauer disk diffusion. RESULTS: There were 115 isolates from 98 patients with acute Otitis media. Gram negative bacteria constituted 66.7% of the isolates. The most common organism was Pseudomonas aeruginosa (34.8%). Others were Staphylococcus aureus (30.4%), Proteus spp (15.7%), Klebsiella spp (11.3%), Escherichia coli (2.6%) and few Fungi (4.1%). Antibiotics sensitivity results of the isolates showed high resistance against most readily available antibiotics. The cumulative resistance of all the bacteria isolates to Augmentin was 89%, gentamicin 80%, ciprofloxacin 34% and ceftazidime 10%. About 88% of the Gram positive bacteria were resistant to penicillin G, amoxicillin, cotrimoxazole, and erythromycin. While 100% of the Gram negative bacteria were resistant to cotrimoxazole, tetracycline, and chloramphenicol. However, commonly isolated organisms were highly susceptible to few 3rd-generation cephalosporins especially ceftriaxone and ceftazidime. CONCLUSION: Based on the result of this study, it is suggested that knowledge of antibiotic profile of etiological agents in Otitis media would be of great advantage in reducing the morbidity and mortality associated with Otitis media.
Clinical application of a bacteriology study on Otitis media
Otitis media (OM) is a notorious inflammation of middle ear that mainly affects tympanic membrane and a major health problem in developing countries causing serious local damage and threatening complications. The focus was mainly on aerobic bacteria, involved in active OM in adults as well as children. An attempt was made, despite resource and man power constraints, to have a glimpse of the current antibiotic sensitivity pattern Early and effective treatment based on the knowledge of causative microorganisms and their antimicrobial sensitivity ensures prompt clinical recovery and possible complications can thus be avoided.
Microbiology Research International
Acute otitis media (AOM) is occasionally caused by Eustachian tube dysfunction and obstruction; it is second most common disease in children. The objective of this study is to determinate and isolate the causative agents of AOM among children and antimicrobial susceptibility pattern to certain antibiotics. A cross sectional hospital based study on 100 acute otitis media patients seen in Khartoum Ear Nose throat Teaching hospital. Patients' information was collected using pre-structural questionnaire. Ear swabs were collected from discharging ears and processed for bacteria culture and susceptibility to different antimicrobial agent using standard microbiological techniques. Out of 100 ear swabs collected and processed through standard microbiological techniques, 68 (total number of culture plate) were culture positive and 32 were negative for bacterial growth. The most common isolated microorganism was Staphylococcus aureus 38 (from total number of isolated organism = 78) (48.7%), followed by Pseudomonas aeruginosa 15 (19.2%), Escherichia coli 4 (5.1%), Citrobacter freundii 8 (10.3%), Morgenalla morganii 9 (11.5%) and Edwardsiella tarda 4 (5.1%). All isolates were resistant to Ampicillin (100%). Almost all isolated bacteria were sensitive to two or more antibiotics. About 6 (15.8 %) of S. aureus were resistant to Methicillin. In conclusions, S. aureus was the major isolated bacteria, about (15.8%) of S. aureus are Methicillin resistance that cause acute otitis media. Ciprofloxacin, Ceftazidime, Gentamicin and Ceftriaxone were relatively effective antibiotics. However, all isolates showed multi drug resistance to the Ampicillin.
IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain
Objective: To isolate and identify various bacterial pathogens in positive ear swab cultures of CSOM cases and to determine the antibiotic sensitivity pattern of different bacterial species identified. Study design: Prospective study Setting: J. L. N. Hospital & Research Centre, Bhilai (C.G). Materials and Method: The study was done on the population of Bhilai and surrounding region. A sample size of 120 cases attending the ENT OPD with the diagnosis of Chronic Suppurative Otitis Media and active ear discharge were included in the study. Detailed history, general physical examination, local systemic examination was done along with aural swab for Gram's staining and aerobic bacterial culture and drug sensitivity. The data were then compared with the relevant & available literature. Results: Most of the patients were in the age group of 11-20 years (38.33%), followed by 21-30 years age group (31.67%). More males (60%) were affected by the disease than females (40%). Unilateral (59.16%) infection was more common than bilateral (40.83%). Incidence of monobacterial (90%) etiology was more common than polymicrobial (9.1%). Staphylococcus aureus 67 (51.53%) was the most commonly isolated bacteria followed by the Pseudomonas 26 (20%). Antibiogram showed the most effective drugs in the present study are clindamycin, cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime Conclusion: Staphylococcus aureus is the most common pathogen isolated, followed by Pseudomonas. Clindamycin was the most effective antibiotic followed by cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime. Antibiotic sensitivity of the organisms varies according to geographical area and local practice regarding the choice of antibiotics. A continuous and periodic evaluation of microbiological pattern and their antibiotic sensitivity pattern in local area is helpful in prescribing empirical antibiotics for successful treatment of otitis media and thus minimizing its complications and emergence of resistant strains.
Journal of Biology Agriculture and Healthcare, 2014
An inflammation of the middle ear by bacteria, viruses or champignons is called otitis media. The objective of this study was to determine the microbial etiology of otitis media in children in Rabat, and susceptibility pattern commonly prescribed antibiotics. Samples of ear swabs were collected from patients at the pediatric hospital, CHU Ibn Sina Rabat, during the period between February 2012 and March 2013. The total number of isolates was 67 (66 bacteria and Candida spp 1). Of these 66 bacteria spp, Streptococcus pneumoniae was the highest number (27) followed by Pseudomonas aeruginosa (16), Staphylococcus aureus (15), Escherichia coli (3), Klebsiella spp (3), Proteus species (2), and candida spp (1). This study out showed that the peak incidence was found in the age group 0-5 years (62,69%), followed by the group 5-10 years (20.90%), by cons, the group age 10-16 years was a rate of 16.41%. All bacteria were resistant to penicillin.. Pseudomonas aeruginosa, Escherichia coli and Klebsiella spp were resistant to certain antibiotics such as ofloxacin, amoxicillin, amoxicillin+clavulanic-acid, levofloxacin and cefuroxime. All strains of streptocoques pneumoniae were sensitive to ciprofloxacin, but all resistant to amoxicillin, cefuroxime and penicillin. All strains of Proteus spp were susceptible to ciprofloxacin, levofloxacin, ofloxacin, only resistant to amoxicillin. Accordingly, sufficient knowledge of the antibacterial susceptibility of bacteria, the judicious use of antibiotics and the periodic evaluation of the resistance profile of antibiotic sensitivity in the region is very important in order to avoid failure in the treatment of otitis media in children.
Impact of multi drugs resistant bacteria on the pathogenesis of chronic suppurative otitis media
African Journal of Microbiology Research, 2010
One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65 (54.2%) males and 55 (45.8%) females, with male: females ratio (1.2:1) and 60 individual without otological problems as control group were included in this study, which done during the period between March 2009 and January 2010. This includes the collection of aural swab samples, culturing of samples, identification of causative agent’s species and antibiotic sensitivity. Gram’s negative bacteria were the commonest microorganism comprises (60%). Pseudomonas aeruginosa was common causative agent (19.04%), followed by Staphylococcus aureus (16.7%) andKlebsiella spp. (14.3%). Mixed infection was found in high percent (74%), in which P. aeruginosa and other microorganisms were more common. The antibiotic sensitivity pattern showed that P. aeruginosa was sensitive to Ciprofloxacin, amoxicillin +clavulanic acid and gentamicin, while other is appeared resistant, S. aureus was sensitive to ciprofloxacin, ...