Polymorphic microsatellite markers for the ant Plagiolepis pygmaea (original) (raw)
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Molecular Ecology Notes
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Conservation Genetics Resources, 2014
Obligate social parasites, or inquilines, exploit the colonies of free-living social species and evolved at least 80 times in ants alone. Most species of the highly specialized inquiline social parasites are rare, only known from one or very few, geographically isolated populations, and the sexual offspring of most inquiline species mates inside the maternal colony. Therefore, inquiline populations are believed to be small and genetically homogeneous due to inbreeding. To comparatively study the genetic diversity of the socially parasitic fungus-growing ant, Mycocepurus castrator, and its only known host species, Mycocepurus goeldii, and to infer the parasite's conservation status, we developed 21 microsatellite markers for the host species, M. goeldii, and evaluated whether these markers cross-amplify in the social parasite, M. castrator.
Polymorphic microsatellite loci in the ponerine ant, Hypoponera opacior (Hymenoptera, Formicidae)
Molecular Ecology Notes, 2005
The ant genus Hypoponera , with its high diversity of alternative reproductive tactics, is of particular interest in studies on sexual selection. In the species Hypoponera opacior , winged and wingless males and queens co-occur and molecular markers are essential to study the reproductive success of these sexual tactics. Primers were developed for five polymorphic microsatellite loci isolated from H. opacior . Their variability was tested on 34 colonies from a population in the United States. Nine to 21 alleles per locus were found with observed heterozygosities between 0.1 and 0.7. A significantly positive F IS value suggests inbreeding in this ant with predominant intracolonial matings.
Research Square (Research Square), 2020
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Isolation and characterization of microsatellite loci from the invasive antPheidole megacephala
Molecular Ecology Resources, 2008
We report the characterization of eight microsatellite markers in the bigheaded ant Pheidole megacephala, a pest ant registered in the list of '100 of the world's worst invasive alien species'. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with 36 individuals collected in an invasive population from Australia and 20 individuals collected in a population from the native mainland location in South Africa. These primers showed a number of alleles per locus ranging from two to 10, and expected heterozygosities ranging from 0.083 to 0.826. Moreover, results of cross-species amplification are reported in five other Pheidole species and in seven other ants of the subfamily Myrmicinae.
Characterization of 12 new microsatellite loci in Aenictus and Neivamyrmex army ants
Molecular Ecology Notes, 2007
Here we describe 12 polymorphic microsatellite loci that were cloned and characterized for three species of army ants: the North American Neivamyrmex nigrescens , and the Asian Aenictus laeviceps and Aenictus dentatus . Observed and expected heterozygosities ranged from 0.37 to 0.97 (mean 0.70), and from 0.48 to 0.95 (mean 0.72), respectively. We observed 2-30 (mean 12) alleles per locus. These new genetic markers will be useful for studies of overall population structure and the complex pedigrees in colonies of army ants.
Molecular Ecology Notes, 2002
Fourteen microsatellite loci were isolated and their level of polymorphism characterized in two populations of the facultatively polygynous plant-ant Petalomyrmex phylax (Formicinae). High levels of within-population variation were observed at most loci, with number of alleles ranging from two to 15, and heterozygosity from 0.050 to 0.925. Cross-species amplification of these loci was also tested in four plant-ant species belonging to three other genera, Aphomomyrmex , Cladomyrma (both Formicinae) and Cataulacus (Myrmicinae).
Microsatellite primers for fungus-growing ants
Molecular Ecology Notes, 2002
We isolated five polymorphic microsatellite loci from a library of two thousand recombinant clones of two fungus-growing ant species, Cyphomyrmex longiscapus and Trachymyrmex cf . zeteki . Amplification and heterozygosity were tested in five species of higher attine ants using both the newly developed primers and earlier published primers that were developed for fungus-growing ants. A total of 20 variable microsatellite loci, developed for six different species of fungus-growing ants, are now available for studying the population genetics and colony kin-structure of these ants.