Antibody Response of Broiler Chickens against Eight Commercial Infectious Bursal Disease Live Vaccines tested by ELISA (original) (raw)
Related papers
Revista Brasileira de Ciência Avícola, 2009
The interference of low or high maternal antibodies titers on the attenuated infectious bursal disease (IBD) virus (IBDV) vaccine infection and its effects on the performance of broilers vaccinated at the 18th day of incubation (in ovo), at one day of age (subcutaneously-SC), or at 15 days of age (drinking water-DW) were investigated. After a series of three live vaccinations, breeders were given or not an IBD oil emulsion vaccine (IBD-OEV) prior to sexual maturity. At day 18 of incubation (in ovo), a commercial vaccine containing HVT and an intermediate IBDV strain or the single HVT vaccine was given. An intermediate IBDV vaccine was given SC at one day of age, or at 15 days of age via DW. The progeny of unvaccinated breeders presented higher neutralizing IBDVspecific antibody (IBDVab) titers at 25 and 40 days of age than those of the progeny of IBD-OEV breeders (p<0.05) at any broilers vaccination age and route. The lower IBDV RNA detection by RT-PCR in the bursa of Fabricius (BF) and the lower IBDV antibody titers in the serum of the groups vaccinated at one and 15 days of age derived from IBD-OEV breeders may indicate antibody-mediated IBDV neutralization. The inovo and one-day vaccinations did not interfere with performance, both in low and high antibody-titered progenies. The in-ovo vaccination against IBD is considered convenient and safe for industrial chickens, irrespective their maternal antibody levels.
International Journal of Poultry Science, 2002
Infectious bursal disease is one of the most important viral disease of poultry usually affects young chickens of 3-6 weeks. Hygienic management and proper vaccination are main way of control of this disease. But maternal antibody affects vaccination with live vaccine. To determine the effect of maternally derived antibody on live vaccine, the study was conducted. A total of 100 day old chicks (50 from vaccinated parent stock and 50 from non-vaccinated parent stock) were used in this study. A preset vaccination schedule was followed for chicken and blood samples were collected to find out the actual effect. It is observed that day old chicks contain high level (6294.14±24.95) of maternally derived antibody which gradually decline below positive level within 15-20 days (390.45 ±19.42) and half-life is about 5 days. Vaccination of chicken with high level of maternally derived antibody interferes with the vaccine virus results no immune response but revaccination provokes immune response. Better immune response is found in chickens vaccinated at day 21 and boostered at day 28. But there may be chance of infection because maternal antibody declined below positive level within 15-20 days. Chickens from nonvaccinated parent stock shows good immune response from first time that is from primary vaccination at day 7 and boosting at day 14.
2018
Infectious Bursal Disease (IBD) is one of the oldest and widely known poultry diseases all over the world. It is caused by IBD virus of Avibirnavirus genus of family Birnaviridae family. IBD has great economical impact on backyard poultry world as it causes high weakness and mortality. The objective of present study was to evaluate the antibody response in village chickens in India after vaccinating them with IBD live vaccine. Serum was collected at regular intervals from chickens up to 112 days after vaccination. Antibodies against IBD virus were measured using ELISA method. It was observed that the vaccines with both intermediate and intermediate invasive strain caused good immune response in the birds. Serum antibody level was found significantly high in 28 days blood collection, which decreased gradually up to 112 days. It was also observed that Intermediate invasive strain produced higher amount of antibodies than intermediate strain of the vaccine. Furthermore, it is also sugg...
Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds
Scientific Reports
Intermediate plus live strain infectious bursal disease virus (IBDV) vaccines are used to control IBDV endemic infections in India. In the present study, immunopathological changes induced by commercial infectious bursal disease live vaccines with different immunization regimes were compared. A total of days old 108 Cobb broiler chicks were randomly divided into five groups with 24 chicks each in groups I, II, III and 18 chicks each in group IV and V. Group I served as control I (no immunization) and group II and III chicks were immunized with a single dose of vaccines 1 and 2 on 17th day of age (DOA), respectively. The group IV and V chicks were immunized with vaccines 1 and 2, respectively with primary dose on 17th DOA followed by booster dose on 24th DOA. Both intermediate plus live vaccines produced gross and histopathological lesions in lymphoid organs (bursa of Fabricius, thymus, spleen and caecal tonsils). Increased CD4 + , CD8 + T cells in affected bursa of Fabricius was evi...
International Journal of Poultry Science, 2012
Collecting sera from newborn chicks requires their sacrifice, which is an invasive and little economic method of sampling. We studied the possibility of replacing the antibody titres in serum of chicks by those in pre-incubated egg yolk for determining the optimal Age of Vaccination (OAV) against Infectious Bursal Disease (IBD) by the Deventer formula. The study focused on 10 hyper-immunized broiler-breeder flocks. Yolk of pre-incubated eggs and sera of newborn chicks, originated from the same parent flock, were essayed by Enzyme-Linked Immunosorbent Assay (ELISA) to detect IBDV specific Maternally Derived Antibodies (MDA). ELISA titres in both types of samples were similar (p<0.05) and well correlated (r = 0.74, p<0.05). The difference between the two vaccination age estimations, based on egg yolk and serum antibodies respectively, was minimal (less than one day). This difference was independent of parent breeder-flock age (r = 0.04, p>0.05 NS). These results encourage the use of egg yolk in predicting the OAV against IBD instead of sera from sacrificed chicks.
One hundred and thirty two, one day old broiler chickens were divided into 4 groups. Group 1 (1 day old) was subcutaneously vaccinated with immune complex vaccine into the nape of the neck. Group 2 and 3 (14 days old) were vaccinated with different commercial products, intermediate-plus strains of live attenuated infectious bursal disease (IBD) vaccine I and II, respectively, via the oral route. Group 4 acted as the positive control. Chickens were bled at days 1, 14, 28 and 38 for evaluating IBD virus antibody titers by ELISA. The body weight, FCR, bursa to body weight ratios and bursal lesion scores at days 14, 28 and 38 were compared. All groups were challenged with local strain of IBD virus at 28 days old. The study revealed that the antibody titers of group 3 were significantly higher (p<0.05) than those of the other groups at day 28. At 38 days old, the body weight of group 2 was significantly lower (p<0.05) than that of group 3. The bursal lesion scores of group 3 were significantly lower (p<0.05) than those of other groups. In conclusion, the immune complex vaccine was shown to be safe for 1 day old vaccination.
Veterinary Research Communications, 2020
Introduction The selection of the right IBD control strategy is primarily based on the choice of the appropriate vaccine strain. High maternal IBD-specific antibodies (Abs) compete with the efficacy IBD vaccine, which necessitates the application of intermediate-plus vaccine strain. Methods A comparative experimental study was designed for evaluation of four different commercially available intermediateplus IBD vaccines in commercial broilers before complete weaning of IBD-specific maternal Abs. Results As determined by IBD-specific quantitative real-time polymerase chain reaction, three tested vaccine strains (228E, Winterfield H2512, and Winterfield 2512) were able to establish in the bursal tissues as early as six hours (hrs) post-vaccination (PV). Both the 228E and the Winterfield H2512 strains vaccinated groups had the highest viral load and replication rate in the bursal tissues at 24, 36, 48 and 72 hrs PV. Earlier seroconversion, 7-14 days PV, was observed in the case of Winterfield H2512, 228E, and Winterfield 2512 vaccinated birds compared to the Lukert vaccinated birds. The 228E strain was more virulent and induces the highest lesion score with severe degrees of lymphocyte depletion and necrosis which persisted up to 28 days PV. Conclusion Overall, the different intermediate-plus IBD strains possess variable early kinetics in the bursal tissues and eliciting antibody (Ab) responses differently withdifferent degrees of bursal lesions. The assessment of the intrabursal vaccine load together with humoral immunity and bursal damage lesion score are fundamental parameters in the evaluation of the intermediate-plus IBD vaccines.
Journal of Agricultural Science and Technology A, 2019
A study on infectious bursal disease virus (IBDV) on chickens of Cobb-500 strain broiler breed at Thakurgaon district of Bangladesh was performed. The protective antibody was measured on one day old chicks (DOC) and post-vaccinated (PV) flocks up to 75 weeks by indirect enzyme linked-immunosorbent assay (I-ELISA). The assays have included five flocks with vaccination historic against IBDV contained 45,000 birds with age ranging from DOC to 75 weeks, just before culling. Maternally derived antibody (MDA) mean titer (MT) ranged from 3,395 to 5,184. The antibody from serum samples (N = 92 per flock) were titer tested by I-ELISA at age 14 d, 5, 8, 23, 50 and 75 weeks of each vaccinated flock. Antibody titer level gradually decreased before vaccination. Vaccination done by intermediate plus vaccine resulting titers level was increased and stayed at the same level. The antibody MT at the 14th day was 500, which supported Deventer method. The protective antibody MT was declined at growing, laying, mid laying and last stage of laying groups. So MDA titer was enough in offspring that could protect birds easily.
Veterinary Medicine : Research and Reports, 2022
Infectious bursal disease (IBD) is one of the most endemic diseases of commercial poultry in Ethiopia. Vaccination is used as the major means of IBD prevention and control. A study was conducted to compare the immunogenicity of two commercially available IBD vaccines in broiler chicken with maternally derived antibody (MDA). Methods: A total of 270 day-one-old chicks were randomly assigned to three groups, group 1 vaccinated with product A vaccine at the age of 7 and 19 days and group 2 with product B vaccine on day 15 and 22 while group 3 were kept as control. Six chickens were also randomly selected and bled on day 1 for differential leukocyte count (DLC) and determination of MDA. Representative chickens from each group were bled at 24th and 42nd days of age for antibody titration using the indirect ELISA test. DLC scores were determined in the 1st and 24th days. Results: The result revealed highly significant differences (P = 0.001) between group 1 and group 2 in DLC at 24th days of age. Antibody titers against IBD were differed significantly (P = 0.02) at 24th and 42nd days of age in broilers vaccinated with product A and product B vaccines. Conclusion: Both vaccines have induced an adequate immunological response at the end of the experiment; however, product A has shown significantly higher antibody titers against the IBDV and DLC than product B.