A new distinct group of 2 S albumins from rapeseed (original) (raw)

Two napins (nla and nlb), isolated from Brrrssicrr rru/~rs (rapcsccd) seeds, have been scqucnccd. The two proteins show the common slrucIural pattern of ~.hc 2 S albumins, since tlq arc composed of Iwo disulfidc-linked chains or dilTcrcn1 size. yet Ihcy exhibit an atypical low molecular weight (12.5 kDa vs. 14.5 kDa of Ihc major napins). High scqucncc similarity has been found bctwcen thcsc 2 proteins. bu1 only 54% similarity can bc cslimaIcd ltom Ihcir comparison wiIh Ihc 14.5 kDir major napins. Thus. nla and nlb arc considcrcd rcprescnIativcs of a new disIinc1 group or rapcsccd napins since all Ihc previously known napinscxhibit 95% scqucncc similariIy. Uncxpccicdly. Ihc similarity incrcascs when compared with [he 2 S prolcins rrom other spccics.