A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute (original) (raw)
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Characterization of sixteen jute genotypes, from Corchorus olitorius L. and Corchorus capsularis L. using jute specific SSR marker attained a high polymorphism value of 92.20%. A total of 171 different alleles were amplified by 27 primer pairs with a mean of 6.33 ± 2.04 alleles per locus. The genetic diversity was also relatively high (0.81 ± 0.06). The Un-weighted Pair-group Method with Arithmetic averages (UPGMA) cluster analysis of the 16 jute genotypes produced a dendogram, which was in concordance with known information. The study reinforces the utility of SSR primers for providing useful and high levels of markers for individual plant genotypes even with a narrow genetic base.
Genetic diversity and relationships in jute (Corchorus spp.) revealed by SSR markers
Bangladesh Journal of Botany, 2010
Characterization of sixteen jute genotypes, from Corchorus olitorius L. and Corchorus capsularis L. using jute specific SSR marker attained a high polymorphism value of 92.20%. A total of 171 different alleles were amplified by 27 primer pairs with a mean of 6.33 ± 2.04 alleles per locus. The genetic diversity was also relatively high (0.81 ± 0.06). The Un-weighted Pair-group Method with Arithmetic averages (UPGMA) cluster analysis of the 16 jute genotypes produced a dendogram, which was in concordance with known information. The study reinforces the utility of SSR primers for providing useful and high levels of markers for individual plant genotypes even with a narrow genetic base.
GENETIC DIVERSITY AND RELATIONSHIPS IN JUTE (CORCHORUS SPP.) REVEALED BY SSR MARKERS
2009
Characterization of sixteen jute genotypes, from Corchorus olitorius L. and Corchorus capsularis L. using jute specific SSR marker attained a high polymorphism value of 92.20%. A total of 171 different alleles were amplified by 27 primer pairs with a mean of 6.33 ± 2.04 alleles per locus. The genetic diversity was also relatively high (0.81 ± 0.06). The Un-weighted Pair-group Method with Arithmetic averages (UPGMA) cluster analysis of the 16 jute genotypes produced a dendogram, which was in concordance with known information. The study reinforces the utility of SSR primers for providing useful and high levels of markers for individual plant genotypes even with a narrow genetic base.
European of agriculture and food sciences, 2023
Indigenous leafy vegetables such as Corchorus olitorius (jew's mallow) have potential in contributing to food security as they can provide important nutritional requirements needed for human nourishment. However, in Botswana, this plant is not yet domesticated and its utilization is minimal due to lack of knowledge. To have the crop fully domesticated it is important to document important information on the available landraces and wild species. One of the important information is understanding the variation among the available genotypes as this will help not only in domestication but also in breeding purposes. Thus this study aimed to assess the genetic diversity of 49 accessions of jews mallow collected in Botswana and other African countries. Simple sequence repeats (SSR) markers were used to characterize fourty nine accessions. The results revealed that, out of 49 SSR primer pairs used, 46 showed scorable polymorphism by producing clear amplified products. The average polymorphic loci rate was 82.44% with the lowest rate (33.33%) detected in MJM-536 and the highest was 100% recorded by 27 of the primers. The polymorphism information content (PIC) potential ranged from 0.398 for MJM-475 primer to 0.979 for the primer MJM-623 with an average of 0.719. With the Shannon diversity index, an average of 3.626 was recorded under the studied accessions and this confirmed a very high diversity. In addition, a dendrogram was generated to illustrate the genetic diversity and possible relationships among the 49 Corchorus olitorius accessions using the unweighted pair group method (UPGM) with arithmetic means. This UPGM cluster analysis distinctively divided the accessions into five (5) groups at a cophenetic correlation coefficient 0.977. The total number of accessions per cluster varied from one group to the other. These results reinforced the effectiveness of the SSR markers in discriminating individuals within species even within a narrow genetic background.
2018
Fifteen inter simple sequence repeat (ISSR) and five simple sequence repeat (SSR) markers were amplified in ninety eight accessions of both the cultivated species of jute Corchorus olitorius and Corchorus capsularis with the objective to study the genetic diversity of both the species at molecular level. Allelic variability, total number of alleles, and higher polymorphic information content (PIC) suggested ISSR as efficient marker system utilized for the study. Jaccard’s similarity coefficients based on ISSR and combined ISSR-SSR analysis gave useful information for appropriate parent selection for future hybridization programme. Principal coordinate analysis (PCoA) supported UPGMA based cluster analysis and species speciation occurred at molecular level.
PTC&B Construction of Genetic Map of Jute (Corchorus olitorius L.) Based on RAPD Markers
2014
The first and preliminary genetic linkage map of the jute genome was constructed with RAPD markers using two parents (Variety O-9897 and Accession No. 1805) and their F2 populations. Linkage analysis at a LOD (Log of odds base 10) score of 3.0 and a maximum distance 50 cM revealed 18 linkage groups. Among the 18 linkage groups, 15 contained single locus and the remaining three groups 16, 17 and 18 contained 2, 11 and 12 loci, respectively. The three multi locus linkage groups varying in length from 15.9- 241.7 cM, snapped a total length of 463.7 cM with an average marker density of 19.6 cM between adjacent markers. The basic chromosome number of Corchorus spp. is seven (2n = 14), so in saturated map, seven linkage groups should have been obtained to represent the genome. But for linkage group analysis, the effort was very limited and the total number of loci (40) was also low.
Construction of Genetic Map of Jute (Corchorus olitorius L.) Based on RAPD Markers
Plant Tissue Culture and Biotechnology, 2009
The first and preliminary genetic linkage map of the jute genome was constructed with RAPD markers using two parents (Variety O-9897 and Accession No. 1805) and their F 2 populations. Linkage analysis at a LOD (Log of odds base 10) score of 3.0 and a maximum distance 50 cM revealed 18 linkage groups. Among the 18 linkage groups, 15 contained single locus and the remaining three groups 16, 17 and 18 contained 2, 11 and 12 loci, respectively. The three multi locus linkage groups varying in length from 15.9 -241.7 cM, snapped a total length of 463.7 cM with an average marker density of 19.6 cM between adjacent markers. The basic chromosome number of Corchorus spp. is seven (2n = 14), so in saturated map, seven linkage groups should have been obtained to represent the genome. But for linkage group analysis, the effort was very limited and the total number of loci (40) was also low.
Genetic diversity in Corchorus olitorius L. revealed by morphophysiological and molecular analyses
Molecular Biology Reports, 2019
Assessment of genetic diversity has an efficient role in plant breeding and improvement programs. There is a limit number of investigations dealing with the evaluation of genetic diversity in Jew's mallow (Corchorus olitorius L.), despite its valuable importance as a leafy vegetable and a delicious dish rich in vitamins and antioxidants. Therefore, in this study, 18 landraces of Jew's mallow-collected from different locations in Egypt-were used for genetic diversity assessment based on morphophysiological and molecular evaluations. A high degree of variability was found among the evaluated landraces at both levels, indicating the appropriateness of such collection to be involved in breeding approaches. Some morphophysiological traits offered a high level of diversity and effectively discriminated the landraces. Thus, they are recommended to be used in successive morphological evaluation studies. On the other hand, molecular evaluation using the random amplified polymorphic DNA (RAPD) and the sequence related amplified polymorphism (SRAP) efficiently supported the morphological results by exposing a clear genetic relationship among the landraces. In addition, the principal coordinate analysis based on combined data of RAPD and SRAP divided the landraces into two main groups, reflecting their relationship molecularly. The first group included nine landraces related to Upper Egypt and the second gathered three landraces from Delta, while the other six landraces were distinctly distributed around these two groups. The two groups may have two distinct ancestors in addition to the different ancestors of the scattered landraces. Findings of this study are valuable and could assist in Jew's mallow breeding programs.
PTC&B Determining Genetic Diversity of Some Jute Varieties and Accessions Using RAPD Markers
2015
The genetic diversity of 18 jute genotypes of the two cultivated species Corchorus capsularis L. and C. olitorius L. which include released varieties and collected races, referred to as accessions was evaluated. DNA profiling was generated using sequence independent RAPD markers. A total of 140 scorable loci were observed and a dendrogram was constructed with these markers. The relationship that is portrayed by this clustering also agreed with the available pedigree information on jute. Two major clusters representing the two species were resolved among the genotypes that were examined in the study. This genetic distance information could be useful in breeding programs in order to introduce agronomically important traits such as short field duration, low temperature tolerance, snow white fibre, higher harvest index etc. From the study one C. olitorius and two C. capsularis varieties were found more suitable for their selection as seed parent against different accessions for improvem...
2022
Genomic DNA (gDNA) from Corchorus olitorius O four was used for the High Throughput Next Generation Sequencing (NGS) podiums. About 50-fold coverage of Jute's genome sequencing data was intended for the recombination assignment. Molecular analysis of repetitive DNA sequences, which account for a large percentage of plant genomes, has not been conducted in jute, but it may be useful for studying chromosome long-range organization. Several open-source and industrial-grade genome assemblage and annotation conduits were used for accumulating and appraise raw statistics. For authenticating the genome project, a transcriptome genome and proteome assessment were additionally also implemented for which evaluated data is assessed by exceptional computing resources, ranging from an overall high-performance cluster server to Dell servers, were used. The jute plant is well adapted to grow in hot and humid climates; however, it is typically grown in a wide range of climatic conditions. Abiotic stress can limit its growth, yield, and quality and affects the metabolism, growth, physiology, and fiber yield of the plant. Despite jute's adaptedness to grow.in hot and humid climates, its growth can be adapted to a wide range of climates and it is relatively resistant to some environmental stresses. However, abiotic stress hinders both jute's growth, yield, and quality significantly. Jute is restricted in its growth, yield, and quality significantly by abiotic stress. Abiotic stress directly affects jute's metabolism, growth, physiology, and fiber yield. However, the utmost proficient array of 858 EST was deposited in the Gene Bank database. Ostensibly, the communal record is far from satisfactory to apprehend the molecular machinery of filamentous biosynthesis. In this review I will cite some of the recent information in the field of sequencing and analysis of Jute (Corchous species) plant by which we can develop jute varieties that are highly productive, saline-tolerant, and produce good quality fibre, including strength and color.