Hepatoprotective Effect of Aqueous Extracts of Some Medicinal Plant Mixtures on CCl4-Induced Liver Toxicity (original) (raw)
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The hepatoprotective action of ten herbal extracts in CCl4 intoxicated liver
Phytotherapy Research, 2005
The effect of ten phytotherapeutic products on CCl4 intoxicated liver in albino male Wistar rats was investigated. Biochemical parameters, including serum transaminase activity (GPT and GOT), histoenzymological measurements (lactate dehydrogenase, LDH; succinate dehydrogenase, SDH, cytochromoxidase, CyOx; Mg2+-dependent adenosine triphosphatase, ATP-ase) and histochemical (Sudan black) and histological examinations (haematoxylin-eosin staining) of the liver were investigated. Some positive effects such as the reduction of hepatocytolysis and steatosis, and a return to normal values of the activity of some enzymes in the following plants: Chrysanthemum balsamita, Echinacea pallida, Calendula officinalis and Corylus avelana were obtained. Copyright © 2005 John Wiley & Sons, Ltd.
GSC Biological and Pharmaceutical Sciences, 2022
Traditional healers combine four medicinal plants (Cochlospermum tinctorium, Terminalia macroptera, Leptadenia hastata and Commiphora Africana to treat hepatitis in Burkina Faso. The aimed was to evaluate the hepato-curative activity of lyophilized aqueous decoction (LAD) and hydroethanolic macerate (LHM) of plant extracts on CCl4-induced hepatitis in rats. We assessed the acute toxicity and scavenging activity of the 2, 2-Diphenyl-1-picrylhydrazyl (DPPH). Hepato-curative activity study included nine groups with five rats each. We used rats as followed: group 1 as neutral controls, group 2 as negative controls, and the other groups were experimental groups. Rats in groups 2-9 received a single dose (1 mL/kg) of CCl4 in intraperitoneal injection to induce hepatitis. We fed orally the rats for seven consecutive days with sylimarin in group 3, LAD and LHM respectively in groups 4-6 and groups 7-9 by 400, 200 and 100 mg/kg/day. This study revealed LAD and LHM had a LD50> 2000 mg/kg a...
Hepatoprotective effect of herbal drug on CCl(4) induced liver damage
Pakistan Journal of Pharmaceutical Sciences, 2013
Herbs have been a huge source of natural substances used to treat and prevent several illnesses; therefore it is vital to identify the probable toxicity that might take place as a consequence of using herbal combinations. This study was undertaken in rabbits to investigate the hepatoprotective effects of herbal drug in normal and CCl 4induced hepatic damage. Herbal drug was tested in 3 different doses, each group comprising of seven rabbits of either sex followed by the administration of CCl 4 with herbal drug and saline for 45 days. Liver function tests and histopathological evaluation were carried out at the end of dosing using standards kits. The result shows that normal dose of herbal drug (0.43 ml/kg) possess hepatoprotective effects against CCl 4 induced liver damage in rabbits which may be due to the various active ingredients present in herbal drug combination. Present study also suggests that there was a significant (p<0.05) increase in serum alkaline phosphatase and γ-GT in animals kept on high dose of herbal drug (10 ml/kg); however studies on huge number of animals and humans are requisite before reaching to definite conclusion.
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF MARKETED HERBAL DRUG PREPARATION
The aim of the present study is to evaluate the protective effect of marketed herbal preparation (Duliv) against carbon tetrachloride (CCl4) induced liver damage in Albino rats. Administration with Duliv Tablet (100mg/kg p.o.)and Duliv Syrup 0.64 ml/100 gm for 15 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, SGPT,SGOT, albumin,protein , cholesterol and alkaline phosphatase.The histopathological studies in the liver of rats also supported that Duliv tablet and Duliv syrup markedly reduced the toxicity of CCl 4 and preserved the histoarchitecture of the liver tissue to near normal. Thus, the results suggest that Duliv preparation acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats.
Evaluation of Hepatoprotective Activity of Traditional Herbal Formulation
sadgurupublications.com
An indigenous herbal formulation containing Cichorium intybus (Wild chicory seed), Sphaeranthus indicus (East Indian globe thistle), Rosa gallica (Red rose petals), Swertia chirata (Chirata), Coriandrum sativum (Coriander), Smilax chinensis (China root), Rheum emodi (Indian rhubarb) and water was investigated for hepatoprotective activity on experimentally induced liver injury with carbon tetrachloride (CCl 4) (0.7 mL/kg body weight, intra peritoneal for 7 days). The hepatoprotective activity of the test formulation was evaluated by the assay of liver functioning biochemical parameters (total bilirubin, serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (SALP) and liver weight). The toxic effect of carbon tetrachloride was controlled significantly by restoration of the levels of total bilirubin, serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (SALP) and liver weight as compared to the normal (control) and the standard drug (Liv52) treated groups. Histology of the liver sections of the animals treated with the test formulation showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity.
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: The leaves of Caesalpinia bonduc (CB) have been used against various disorders in folk medicine including the liver disorders. Earlier, we have shown the hepatoprotective effect of CB in acute hepatotoxicity model. The present study was designed to evaluate the anti-hepatotoxic and anti-fibrotic effect of the aqueous leaf extract of CB on CCl4 (carbon tetrachloride) induced chronic hepatotoxicity/fibrosis in Wistar rats. Methods: Animals were divided into three groups namely; preventive, curative and prophylactic, which was further subdivided into four groups each: Group I-untreated control, group II-CCl4 control, group III-CB+CCl4 and group IV-silymarin+CCl4. The aqueous extract of CB/silymarin was administered orally once, daily for eight weeks in the curative group and for four weeks in preventive and prophylactic groups respectively. The chronic liver damage/fibrosis was induced by intraperitoneal injection of CCl4 twice a week, for four weeks in preventive and prophylactic groups and for eight weeks in the curative group. Blood samples were collected for assaying serum biochemical parameters, and the livers were excised and processed for histology. Results: The data showed that supplementation of aqueous leaf extract of CB along with CCl4 significantly reduced the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase(ALP), total bilirubin(TB) and prothrombin time(PT) thus further restoring the total protein(TP) and albumin(ALB) in preventive, curative and prophylactic groups when compared to CCl4 control. Significant improvement in the microscopic structure of the liver further confirmed the hepatoprotective effect of aqueous extract of CB over the liver injury and fibrosis induced by CCl4 in rats. Conclusion: The study, therefore, suggests that aqueous extract of CB might provide a novel and alternative approach for treating the chronic hepatotoxicity/liver fibrosis.
Effects of few Indian medicinal herbs on carbon tetrachloride induced hepatic injury in animals
2009
The Phyllantus nirruri (PN), Andrographis paniculata (AP) and Picrroriza kurroa (PK) have been widely used in number of hepatoprotective formulations based on their traditional claims. However, no published data showing the comparative study of these herbs is available. The aim of the present study was to evaluate the hepatoprotective effect of standardised herbal extracts of PN 142.5 mg/kg, p.o), AP (300 mg/kg, p.o) and PK (200 mg/kg, p.o) on carbon tetrachloride (CCI 4) induced acute and chronic hepatic damage in rats. In chronic hepatitis, CCI 4 (0.2 ml/kg, p.o) was administered twice weekly during eight weeks of extract treatment, whereas, in acute hepatitis, CCI 4 (0.5 ml/kg, p.o) was given on 10 th day of administration of extract. Liver injury was analyzed by estimating the biochemical marker enzymes levels and antioxidant activities. Histological studies were also carried out. Statistically significant reversal of the elevated serum levels and depleted tissue levels of biochemical marker enzymes were found as the biochemical indices for hepatoprotection. The histological scores and liver weight was significantly reduced as well as SOD and catalase activities were significantly increased in treated groups compared to CCI 4 control. Further, PK and PN were found to be most and least effective respectively in chronic CCI 4 induced liver damage, whereas, AP exhibited least protection than PN and PK in acute CCI 4 hepatic injury. These results show that the standardised extracts PN, AP and PK possess hepatoprotective potential and prevented hepatic damage induced by CCI 4 .
Pharmacognosy Communications, 2017
Background and Aim: Herbal remedies offer another strategy for alleviation of co-existing liver and kidney pathologic conditions. Objective: The present study ascertained the functional integrity of hepatic and renal tissues, concurrently with blood lipid patterns, of Wistar rats infused with CCl 4 and treated with raw and hydrothermal processed herbs, namely, Monodora myristica, Chromolaena odorata, Buccholzia coriacea and Sphenostylis stenocarpa. Materials and Methods: Measurement of phytochemical contents of the herbs was according to standard methods. The rats were randomly designated on the basis of diets and treatments received for 28 consecutive days. Fibrosis was induced in the Wistar rats by single dose intra-peritoneal injection of CCl 4 for 2 consecutive days. Liver and kidney function tests and serum lipid profile were measured using spectrophotometric methods. Renal and hepatic tissues were subjected to histopathological examinations. Results: The concentrations of alkaloids in the four herbal extracts were within the range of 4.83 ± 0.03-31.33 ± 0.29 mg/100 g sample, whereas the concentrations of saponins varied within a relatively narrow range: 0.33 ± 0.09-4.33 ± 0.02 mg/100 g dry sample; p > 0.05. The activity ratios of AST to ALT of the rat groups were generally less than 1.0 unit. Atherogenic indices of fibrotic rats were within the following ranges: TAG/HDL-C ratio (3.59 ± 0.03-6.76 ± 0.06), TC/HDL-C ratio (3.72 ± 0.02-6.94 ± 0.05) and LDL-C/HDL-C ratio (2.00 ± 0.01-4.59 ± 0.02). Conclusion: Losses in phytochemical contents following hydrothermal processing of the herbs did not substantially affect their overall therapeutic scores against morphological and functional impairments of hepatic and renal tissues following CCl 4 intoxication of the rats.
The methanol extracts of plant materials of some plants like Casuarina equisetifolia, Cajanus cajan, Glycosmis pentaphylla, Bixa orellana, Argemone mexicana, Physalis minima, Caesalpinia bonduc, belonging to the different family were studied for hepatoprotective activity against Swiss albino rats with liver damage induced by carbon tetrachloride(CCl). It was found that the methanol extract of B. orellana, C. cajan, 4 G.pentaphylla and C. equisetifolia at a dose of 500 mg/kg body weight exhibited moderate protective effect by lowering the serum levels of alanine aminotransferase(ALT) or Serum Glutamate Pyruvate Transaminase (SGPT), aspartate aminotransferase (AST)or Serum Glutamate Oxaloacetate Transaminase (SGOT) and cholesterol to a significant extent. Other methanol extracts of A. mexicana, P. minima and C. bonduc had no effect of lowering blood serum level rather than produced toxicity at the above specified dose. The highest activity of observed for methanol extract of B. orellana at a dose of 500 mg/kg body weight (b.wt.) and the reduction of serum level of ALT, AST and cholesterol were 52.08%, 57.37% and 52.90% respectively. The hepatoprotective activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of carbon tetrachloride treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by CCl and blood samples from the animals 4 treated with the methanol extracts of B. orellana, C. cajan, G.pentaphylla and C. equisetifolia showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells, the extracts of four above plants could afford significant dose-dependent protection against CCl induced hepatocellular injury. 4
ABSTRACT The liver is a vital organ in the body. It plays a major role in metabolism, including ridding the body of substances that would otherwise be injurious if allowed to accumulate, and excretion of xenobiotics from the body. The endogenous antioxidants defenses from reactive oxygen species are strengthened by natural antioxidants and restore the optimal balance by neutralizing reactive species. The present study aims to highlight on hepatotoxic agents, and prevention of hepatic disorders using Curcuma longa, Trigonella foenumgraecum, Allium sativum, Coffea arabica, Petroselinum crispum, Olea europaea leaves, and Mentha piperita. Curcuma longa showed that hepatoprotective effect against hepatotoxicity induced by paracetamol, diethyl nitrosamine, CCl4, and gentamicin. Also, the hepatoprotective effect of Trigonella foenumgraecum seeds has been elucidated against hepatic disorders induced by γ-radiation, monosodium glutamate, ethanol, CCl4, AlCl3, and diabetes. Allium sativum has been used in the treatment of hepatic disorders. The hepatoprotective effect of Allium sativum has been confirmed against oxidative damage and hepatic toxicity of D-galactosamine, lipopolysaccharide, ethanol, and CCl4, which may be due to the presence of organosulfur compounds. Coffea arabica intake has been inversely related to the incidence of liver diseases. Petroselinum crispum showed a hepatoprotective effect against hepatic disorders induced by CCl4 and diabetes, which may be due to their high content of antioxidants. The leaves of Mentha piperita showed a good hepatoprotection against hepatopathy induced arsenic, anti-tuberculosis drugs, and CCl4 which may be due to their high content of phenolics and flavonoids. Olea europaea leaves extract significantly ameliorated pathophysiological changes induced in the liver by diazinon, carbendazim, and γ-irradiation in rats. It can be concluded that administration of Curcuma longa, Trigonella foenumgraecum, Allium sativum, Coffea arabica, Petroselinum crispum, Olea europaea leaves, and Mentha piperita showed a remarkable hepatic protection against hepatotoxic agents, which may be due to its antioxidant properties of these medicinal plants and herbs. So, Human expose to hepatotoxic agents and the patients with hepatic disorders should be advised to take these medicinal plants and herbs.