HIV-1 gp160 Induces Transforming Growth Factor-β Production in Human PBMC (original) (raw)

1996, Clinical Immunology and Immunopathology

The mechanisms by which HIV infection results in Transforming growth factor-b (TGF-b) is a multiinduction of TGF-b secretion is however unknown. The functional cytokine secreted by many mononuclear HIV envelope glycoprotein gp160, which is expressed cells in peripheral blood (PBMC) and has diverse efon the surface of HIV and of HIV-infected cells, interfects on cellular and humoral immunity. Increased acts with the CD4 receptor of CD4-bearing T cells and TGF-b mRNA expression has been reported in PBMC macrophages with high affinity and has been shown of HIV-infected patients, but the mechanism by which to induce a variety of cytokines from T cells and/or HIV induces TGF-b secretion is unknown. In this macrophages (9-11). We have previously demonstudy, we observed that HIV gp160 could induce sigstrated that gp120/gp160 could induce IL-6/GM-CSF/ nificant TGF-b secretion and TGF-b mRNA expression IL-3 mRNA expression and cytokine secretion in pein PBMC from HIV-seronegative healthy donors. The ripheral blood mononuclear cells (PBMC), CD4 / T cell cellular source of TGF-b was attributed to non-T cells, clones, and cord blood T cells (12, 13). Other groups presumably monocytes. Specificity of secreted TGF-b have shown that gp120 can induce IL-1b and TNF-a was confirmed by the addition of anti-TGF-b mAb in macrophages as well (14). In the present study, we which abrogated the proliferative response of CCL-64 have investigated the influence of the highly purified cells by gp160-treated culture supernatants. Soluble native gp160 in inducing TGF-b production. Here, we CD4 blocked the gp160-induced TGF-b production, demonstrate that in vitro exposure of normal PBMC to suggesting that CD4-gp160 interaction is required to induce TGF-b production. Our results suggest that gp160 results in the induction of TGF-b mRNA expres-HIV-1 gp160 may contribute to the immune defects in sion and secretion of bioactive molecules. Soluble CD4 HIV infection by inducing TGF-b secretion. ᭧ 1996 blocked the gp160-induced TGF-b production, sug-Academic Press, Inc.