Influence of imidacloprid treatment on Protein Profiling of soil isolate Bacillus weihenstephanensis (original) (raw)
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Bulletin of Environment, Pharmacology and Life Sciences, 2023
Imidacloprid (IMI), a neonicotinoid-class synthetic organic insecticide, is widely used across the world to control both piercing and sucking insect pests. It can persist in soil and contaminate soil, ground water etc. Microbial bioremediation aids in enhancing the soil's health by transforming dangerous pesticide byproducts into less toxic metabolites. The primary screening and isolation of microbes was done by enriching contaminated soil in a carbon-limited liquid Mineral Salt Medium (MSM) containing 100 ppm IMI concentration. Total eleven bacterial strains were isolated and were screened further for their tolerance to proliferate on MSM agar plate containing possible maximum concentration (50,000 ppm) of IMI. Four bacterial isolates VG5, VG7, VG 10 and VG11 were grown fast at highest IMI concentration and were screened further for growth pattern study in full strength MSM medium. Based on the growth pattern and biomass production isolate VG5 was selected as the most efficient strains for the IMI degradation study. Isolates VG5 was characterized on the basis of morphological, cultural and biochemical characteristics as Bacillus sp. by referring Bergey's Manual of Determinative Bacteriology. This potential isolates was identified by 16S rDNA gene sequencing as Cytobacillus firmus.
2018
Pesticides are the chemical substances that prevent, kill, repel any pest. Repeated use of same pesticide, bulky handling or accidental release results in accumulation of pesticides residues or its metabolites in soil or water streams. The persistent nature of pesticides carries potential hazards to man and show lethal effects on living system. Imidacloprid (1-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine), is a second-generation neonicotinoid pesticide (chloronicotinyl insectile). It is used to treat a wide range of pests on rice, maize, potatoes and vegetables. Imidacloprid has been reported as a stable compound in the environment with a half-life over 100 days. There are very few repots of imidacoprid degrading soil microorganisms. In an effort to find active microbial strains over the problem of commonly used pesticides like imidacloprid, a study was carried out. The objectives of this study were to isolate and characterize the efficient imidacoprid degrading micro...
Imidacloprid application changes microbial dynamics and enzymes in rice soil
Ecotoxicology and environmental safety, 2017
Extensive use of imidacloprid in rice ecosystem may alter dynamics of microorganisms and can change soil biochemical properties. The objective of this study was to assess the effect of imidacloprid on growth and activities of microbes in tropical rice soil ecosystem. Four treatments, namely, recommended dose (at 25g a.i. ha(-1), RD), double the recommended dose (at 50g a.i. ha(-1), 2RD), five times the recommended dose (at 125g a.i. ha(-1), 5RD) & ten times the recommended dose (at 250g a.i. ha(-1), 10RD) along with control were imposed under controlled condition. Dissipation half lives of imidacloprid in soil were 19.25, 20.38, 21.65 and 33.00 days for RD, 2RD, 5RD and 10RD, respectively. In general bacteria, actinomycetes, fungi and phosphate solubilising bacteria population were disturbed due to imidacloprid application. Changes in diversity indices within bacterial community confirmed that imidacloprid application significantly affected distribution of bacteria. Total soil micro...
PloS one, 2017
In this study, different context-dependent effects of imidacloprid exposure on the honey bee response were studied. Honey bees were exposed to different concentrations of imidacloprid during a time period of 40 days. Next to these variables, a laboratory-field comparison was conducted. The influence of the chronic exposure on gene expression levels was determined using an in-house developed microarray targeting different immunity-related and detoxification genes to determine stress-related gene expression changes. Increased levels of the detoxification genes encoding, CYP9Q3 and CYT P450, were detected in imidacloprid-exposed honey bees. The different context-dependent effects of imidacloprid exposure on honey bees were confirmed physiologically by decreased hypopharyngeal gland sizes. Honey bees exposed to imidacloprid in laboratory cages showed a general immunosuppression and no detoxification mechanisms were triggered significantly, while honey bees in-field showed a resilient re...
Biodegradation of Imidacloprid, the New Generation Neurotoxic Insecticide
2020
ABSTRACT: Imidacloprid (1-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine), a chloronicotinyl insecticide used to control biting and sucking insects, is very persistent in the soil with a half-life often greater than 100 days. Although a few soil metabolites have been reported in the literature, there are few reports of biodegradation of imidacloprid. Our objectives were to discover, isolate, and characterize microorganisms capable of degrading imidacloprid in soil. Two soil free stable enrichment cultures (NUS1, and NUS4) in minimal media were obtained that showed maximum degradation of Imidacloprid between 48 -72 hours after incubation. The degradation was indicated by growth of microorganisms in minimal media, where sole source carbon and nitrogen was Imidacloprid. The degradation product was characterized by High Performance Liquid Chromatography (HPLC), which was found to be 6-Chloronicotinic acid. The two isolates were thus found to metabolize Imidacloprid and were...