Collagen metabolism in normal and complicated pregnancy: changes in the aminoterminal propeptide of type III procollagen in serum (original) (raw)
Related papers
American Journal of Biomedical and Life Sciences
It was a prospective study with a case control design. The purpose of the study was to measure the urinary collagens IV (U-coll IV) and microalbumin (MA) level in early pregnancy and to explore the role of excretion U-coll IV in prediction of preeclampsia (PE). A total number of 119 pregnant women at 10-14 weeks of pregnancy were selected on the basis of availability. MA by immunoturbidimetry assay and U-coll IV by enzyme immunoassay method were measured in these subjects and they were followed up to the term for the possible development of PE. MA was defined by albumincreatinine ratio (ACR) above 32mg/g and high U-coll IV was defined by values above the cutoff point 2.74 ng/ml determined by the median value of the (controls). The data were analyzed by grouping the subjects who developed PE in later stages of pregnancy (the PE group) and those who did not develop PE in later stages (Control group). From the total subjects, 10 developed PE which shows a prevalence of about 8.4%. The PE group had higher value of ACR as compared to Control [ACR, mg/g, median (range), 42.3]. The sensitivity of ACR in predicting PE was 80%, specificity 49.54%, PPV 12.69% and NPV 96.42% respectively. The sensitivity of high U-coll IV in predicting PE was 70%, specificity 50.5%, PPV 11.5% and NPV 94.8%. It may be concluded that early pregnancy levels of MA and U-coll IV can be used as predictors of PE with high negative predictive value; and U-coll IV has no added advantage over MA in this respect.
Folia Medica
Introduction: Collagen type I is a major structural component of human uterus. Recent studies have found that collagen type I turnover is altered in preeclampsia and imbalance occurs between collagen synthesis and degradation processes. This acts as a stimulus for abnormal changes in the concentrations of collagen type I derived products in circulating blood. Aim: Having in mind the pathological collagen type I turnover in preeclampisa, the aim of the present study was to determine the levels of N-terminal propeptide of collagen type I (PINP), C-terminal propeptide of collagen type I (PICP): both markers of collagen type I synthesis and levels of matrix metalloproteinase-1 (MMP-1): marker of collagen type I degradation in sera of women with early-onset preeclampsia. Materials and methods: Thirty-two patients with early-onset preeclampsia were examined. The mean age of the patients was 28.8±5.5 years; and the mean age of 22 age-matched healthy pregnant women was 28.5±6.0 years. The e...
The concentration of collagen and the collagenolytic activity in the amnion and the chorion
European Journal of Obstetrics & Gynecology and Reproductive Biology, 1989
obtained from second-trimester abortions, elective Caesarean sections and after normal deliveries were studied. The hydrolytic activity against a DNP-peptide expressing the collagen turn-over was found to be very high in the second trimester and relatively high at term of pregnancy. The activity in the chorion was twice that in the amnion. The concentration of another collagen-degrading enzyme, leukocyte elastase, which is present during inflammatory reactions was high at the site of rupture.
European Journal of Obstetrics & Gynecology and Reproductive Biology, 2001
Objectives: To investigate the concentrations of osteocalcin and collagen type I C-terminal telopeptides in pregnant women with preeclampsia. Study design: 26 patients with severe pre-eclampsia and 24 healthy pregnant women were included in the study. Serum concentrations of osteocalcin and C-telopeptides Ð degradation products of type I collagen were determined using the ELISA method. Statistical analysis was performed using Mann±Whitney U-test. Results: In pre-eclamptic patients, the concentrations of osteocalcin and degradation products of collagen type I were signi®cantly higher (P < 0:005) when compared to healthy pregnant women. Conclusion: These results could suggest that there are alterations in bone metabolism in pregnant women with pre-eclampsia.
American Journal of Obstetrics and Gynecology, 1990
We have previously found the serum concentration of the aminoterminal propeptide of type III procollagen, an indicator of collagen metabOlism, to be increased in advanced ovarian cancer. In this study we measured the serum aminoterminal propeptide of type III procollagen concentration in healthy women during the menstrual cycle and in patients with salpingo-oophoritis, leiomyomas, endometriosis, and benign ovarian tumors. The concentration was higher in the luteal phase than that in the follicular phase, suggesting an association of collagen metabolism with ovarian steroid hormones. Severe salpingo-oophoritis increased the serum level of the aminoterminal propeptide of type III procollagen with a decrease to normal during recovery. Elevated values were occasionally seen in endometriosis and leiomyomas. These findings indicate that the aminoterminal propeptide of type III procollagen is a relatively unspecific indicator of ovarian carcinoma. (AM J OBSTET GVNECOL 1990;163:1276-81.) Key words: Menstrual cycle, endometriosis, ovarian cysts, salpingo-oophoritis, aminoterminal pro peptide of type III procollagen Type 111 procollagen is one of the most abundant constituents of interstitial connective tissue, and comprises about 20% of the collagenous framework of both the myometrium and the cervix. I It is synthesized in the form of a larger precursor protein, known as type III procollagen, which contains additional peptide extentions at both ends. These sequences are known as the aminoterminal and carboxy terminal propeptides of type III procollagen.
Amniotic fluid laminin and type IV collagen in normal and pathological pregnancies
Clinica Chimica Acta, 1985
Specific radioimmunoassays for the fragment Pl of human laminin and the 7-S collagen domain of human type IV collagen were used to quantify these basement membrane proteins in second trimester amniotic fluid samples from 21 normal and 41 patholo~c~ pregnancies, the latter group being defined by elevated amniotic fluid cu-foetoprotein (AFP) or abnormal foetal karyotype, or both. The mean laminin PI concentration in the normal 15 to 18-wk pregnancies was 36 pg/l (range 10-77) and that of the 7-S collagen was 46 pg/l (range 7-152). The molecular size of the antigens in amniotic fluid from both normal and pathological pregnancies, when assessed by gel filtration was very large, probably representing intact laminin and type IV collagen. Pathological pregnancies, e.g. cases of Turner syndrome, Meckel syndrome and anencephaly often had elevated amniotic fluid laminin and type IV collagen con~ntrations. A weak, but nevertheless significant, correlation was found between the amniotic fluid laminin and type IV collagen concentrations and also between type IV collagen and AFP, but none between laminin and AFP. In eight pregnancies with foetuses suffering from the congenital nephrotic syndrome of the Finnish type, a genetic disease assumed primarily to involve some component of the glomerular basement membrane, the arrmiotic fluid concentrations of both laminin and type IV collagen were within normal limits in spite of an elevated amniotic fluid AFP.
Biomedical Research, 1987
A neutral proteinase catalyzing the removal of the C-terminal cross-linking region of type I collagen (telopeptidase) was extracted from rat uteri obtained 0-5 days postpartum. This enzyme showed lack of latency, in contrast to the collagenase which was activated by trypsin. Telopeptidase activity was highest at 0 and 1 day postpartum and decreased rapidly to the nulliparous level by 3 days. Collagenase activity increased slightly from 0 to 1 day postpartum and decreased rapidly thereafter. The elevated levels of these two enzymes were correlated with rapid loss of uterus collagen (77% by 3 days postpartum). The extracted telopeptidase had a neutral pH optimum. The major fraction isolated by gel-chromatography had an apparent molecular weight of approximately 15,000. It was inhibited by ethylenediamine-tetra-acetic acid and 1,10-phenanthroline but not by N-ethylmaleimide, nor by phenylmethanesulphonyl fluoride. It is therefore probably a metalloproteinase. 3
Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
International Journal of Morphology, 2011
The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was undertaken to analyze the rol of osteopontin, fibrinogen and collagen in uterine-placental interface during normal porcine gestation. Uterine and placental tissues from crossbred gilts of 30 (n=5), 60 (n=5), 70 (n=5) and 114 (at term, n=5) days of gestation were used. Macroscopic analysis of the embryos/fetuses allowed us to determine their gestational age by means of the crown-rump lenght. Haematoxylin-Eosin and Masson's Trichrome dyes along with light microscopy were used to structure analysis of every selected period of gestation. A spacial and temporal study of osteopontin and fibrinogen was performed through immunohistochemical technique. Determination of collagen fibers was carried out through Picrosirius red technique and polarizing microscopy. Results were expressed as semi-quantitative. Higher expression of osteopontin was observed at early periods of gestation, mainly in uterine and placental villi, endometrial gland epithelium and histotroph. Fibrinogen expressed abundantly in fetal mesenchyme in every period analyzed and in fetal and maternal vessels at Day 70. Negative expression of collagen fibers was observed in villi, however increasing expression of thick fibers throughout pregnancy was detected in uterine stroma and myometrium. These results confirm the importance of osteopontin, fibrinogen and collagen in the support of uterine and placental structures and in the suitable maintenance of pregnancy.
Archives of Gynecology and Obstetrics, 2011
Purpose To compare the maternal serum and placental tissue levels of prolidase enzyme activity in women with early pregnancy loss (EPL) with those of women who underwent elective dilatation and curettage (D&C). Methods Serum and placental tissue samples from 52 women with early pregnancy loss (study group) and 49 women who underwent elective D&C (control group) were collected. Criteria for inclusion in the study group were based on evidence of vaginal bleeding, transvaginal ultrasonography results, and clinical findings. Prolidase enzyme activity was determined using a photometric method. Results Serum levels of prolidase activity were lower in the early pregnancy loss group than in the control group (p \ 0.001). However, placental tissue activity levels were significantly higher among women with an EPL than among women without an EPL (p \ 0.001). Conclusion Results from this study demonstrate an inverse relationship of serum and placental levels of prolidase activity. Placental utilization of the prolidase enzyme may explain the low serum levels. As prolidase is a marker of collagen turnover, we conclude that collagen turnover is increased in patients with early pregnancy loss and may be an etiopathological factor of this disease.