Two new pregnane-type steroidal alkaloids from Sarcococca saligna (original) (raw)
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The bioassay-guided phytochemical investigation of Sarcococca hookeriana with respect to cholinesterase inhibitory properties has yielded two new 5R-pregnane-type steroidal alkaloids, hookerianamides J (1) and K (2), along with eight known compounds (3-10). The structures of 1 and 2 were elucidated by spectroscopic methods. These compounds displayed good to moderate activities in vitro against the enzymes acetylcholinesterase (IC 50 8.1-48.5 µM) and butyrylcholinesterase (IC 50 0.4-4.0 µM). Compounds 1-10 were also tested in vitro for their leishmanicidal activity against Leishmania major and for their antibacterial activities against Bacillus subtilis, Micrococcus luteus, Streptococcus faecalis, and Pseudomonas pallida.
TWO NEW PREGNANE-TYPE STEROIDAL ALKALOIDS FROM SARCOCOCCA SALIGNA Key Word Index--Surcococca
Buxaceae; steroidal alkaloids; (2OS, 2'Z)-20-(N,N-dimethylamino)-38-(2-methyl-2Z-butenamidone; (2OS, 2'Z)-20-(N,N-dimethyl-amino)-3/&2-(methyl-2Z-butenamido)-pregna-5, 14-dien-4-one. Abstract-Two new preganae-type steroidal alkaloids, (2OS, 2'Z)-20-(N,N-dimethylamino)-3B-(2-methyl-2Z-butenamido)-pregn-5-en-4-one and (2OS, 2'Z)-20-(N,N-dimethylamino)-3~-(2-methyl-2Z-butenamidc~)-pregna-5,14-dien-4-one, have been isolated from an ethanolic extract of the roots and stems of Surcococca saligna, in addition to the two known alkaloids, N-formylchonemorphine and vaganine-A, which have been isolated for the first time from this species. 0 1997 Elsevier Science Ltd INTRODUCTION
The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens
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The growing incidence of microorganisms that resist antimicrobials is a constant concern for the scientific community, while the development of new antimicrobials from new chemical entities has become more and more expensive, time-consuming, and exacerbated by emerging drug-resistant strains. In this regard, many scientists are conducting research on plants aiming to discover possible antimicrobial compounds. The secondary metabolites contained in plants are a source of chemical entities having pharmacological activities and intended to be used for the treatment of different diseases. These chemical entities have the potential to be used as an effective antioxidant, antimutagenic, anticarcinogenic and antimicrobial agents. Among these pharmacologically active entities are the alkaloids which are classified into a number of classes, including pyrrolizidines, pyrrolidines, quinolizidines, indoles, tropanes, piperidines, purines, imidazoles, and isoquinolines. Alkaloids that have antio...