Dietary α-linolenic acid from flaxseed oil or eicosapentaenoic and docosahexaenoic acids from fish oil differentially alter fatty acid composition and characteristics of fresh and frozen-thawed bull semen (original) (raw)
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SLOVENIAN VETERINARY RESEARCH, 2019
The aim of this work was to evaluate the effects of flaxseed oil supplementation as a source of omega-3 fatty acids (OFA) on some blood parameters, semen quality and testosterone level in male Frisian bull. A total of 30 Friesian bulls (14.2 ± 0.16 month of age and 265±15 kg body weight) were divided into three equal groups according to age and live body weight. Bulls in the 1 st group (G1) were supplemented with 2% dray matter intake (DMI) flaxseed oil as a source of OFA, while those in 2 nd group were supplemented with 4% DMI flaxseed oil as a source of OTA at 14 to 21 months of age (G2). Bulls in the 3 rd group were fed a basal diet and considered as a control group (G3). The obtained results revealed that OFA administrated animals had a significant (P< 0.05) inducing effect on serum levels of total protein, globulin, glucose, high density lipoprotein (HDL) and triglyceride compared to the control group. No significant changes were noticed in albumin levels among the three groups. However, the levels of total cholesterol, urea and low density lipoprotein (LDL) were significantly lower in bulls received OFA than the control group. Omega-3 fatty acids treatment has a significant positive effect on the semen characteristics and lower abnormality in G2 and G3 than in G1. G2 and G3 also showed a significant higher intact spermatozoa cell membrane than in G3 by about 9.68 and 10.84%, respectively. Omega-3 treatment significantly increased blood testosterone levels to be 23,78% in G2 and 31.82% in G3 higher than that of the control. In conclusion, dietary supplementation with omega-3 in male Frisian ration improved semen quality and reproductive potentiality as well as testosterone level.
Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm
Animal, 2010
The fatty acid composition of sperm affects the fertilization rate. The objective was to investigate the effects of dietary fish oil (as a source of n-3 fatty acids) on semen quality and sperm fatty acid composition in sheep. Eight Zandi fat-tailed rams were randomly allocated into two groups and fed either a control diet or a diet supplemented with fish oil. Both diets were isocaloric and isonitrogenous and were fed for 13 weeks, starting in the middle of the breeding season. Semen samples were collected weekly and their characteristics evaluated by standard methods, whereas samples collected at the start and end of the study were assessed (gas chromatography) for sperm lipid composition. Mean (±s.e.m.) sperm concentrations (4.3 × 109± 1.3 × 108v. 3.9 × 109± 1.3 × 108sperm/ml and percentages of motile (77.25 ± 3.34v. 60.8 ± 3.34) and progressively motile sperm (74.13 ± 1.69v. 62.69 ± 1.69) were significantly higher in the fish oil group than control. Dietary fish oil increased the ...
The Professional Animal Scientist, 2009
The use of cooled and frozen semen offers many advantages to breeders. However, many stallions produce spermatozoa that are unable to endure the stresses associated with these preservation processes. Improving the quality and viability of equine spermatozoa via appropriate dietary alterations may render spermatozoa from these stallions commercially viable for preservation. One way to improve sperm quality may be through increasing dietary intake of PUFA, specifically docosahexaenoic acid (22:3n-3), to enhance the plasma membrane of the sperm cell. To evaluate this possibility, along with analyzing varying supplemental sources of n-3 fatty acids that are high in docosahexaenoic acid, stallions were placed into 1 of 2 treatment groups and fed either an algae-and flaxseed-based or a fish-based dietary supplement. Semen was collected every 2 wk for 112 d, 28 d before and 84 d during PUFA supplementation. Spermatozoa motion characteristics and membrane integrity and morphology of fresh, cooled, and stored (24 and 48 h), and frozen and thawed semen samples were analyzed. When spermatozoa obtained from stallions in each treatment were compared, no overall improvements were found (P > 0.05) in motility, percentage of membrane-intact spermatozoa, and percentage of morphologically normal spermatozoa regardless of supplementation or source of supplementation.
Animals, 2021
The demand to conserve indigenous species through the cryo-gene bank is increasing. Spermatozoa remain sensitive to cryopreservation damages especially that of avian species thus limiting the use of reproductive biotechnologies such as artificial insemination in the conservation programs. Long-chain polyunsaturated fatty acid (LCPUFAs), specifically omega n-3, expanded a research interest to improve animal reproductive efficiency through improving spermatozoa quality. This is driven by the fact that mammals cannot synthesize omega-3 de-novo because they lack delta-12 and delta-15 desaturase enzymes thus supplemented in the diet is mandatory. Delta-12 and delta-15 add a double bond at the 12th and 15th carbon-carbon bond from the methyl end of fatty acids, lengthening the chain to 22 carbon molecules. Fish oil is a pioneer source of omega n-3 and n-6 fatty acids. However, there is a report that numerous fisheries are over-exploited and could collapse. Furthermore, processing techniqu...
Veterinaria México OA, 2017
The aim of this study was to evaluate the effects of adding conjugated linoleic acid (CLA) to boar diets on semen producion and characteristics, the fatty acid profile of spermatozoa, and on testicle and boar carcass parameters. Ten lean-type terminal-line (Yorkshire x Landrace x Petrain) adult boars were randomly assigned to one of two treatments: (1) a control diet and (2) the same diet with 1% CLA. The research was conducted in Mexico, and the animals were exposed to ambient temperatures betwen 13.5 and 26.3 °C. Boars were ejaculated twice per week a 12-week period to examine semen production and characteristics. The results suggest that supplementation of boar diets with CLA did not affect the spermatozoa fatty acid profile, semen volume, sperm concetration, viability, or motility, nor did it cause abnormal sperm morphology. Compared to controls, boar fed a CLA diet had a higher seminal pH and increased backfat thickness. In summary, this study indicates that CLA addition in boa...
International Journal of …, 2011
Background: Long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family are important for sperm membrane integrity, sperm motility and viability. There are evidences to suggest that dietary supplementation with omega-3 fatty acids affects reproduction in men and males of different animal species. Therefore, the aim of current study was to investigate changes in the quality parameters of Holstein bull semen during heat stress and the effect of feeding a source of omega-3 fatty acids during this period. Materials and Methods: Samples were obtained from 19 Holstein bulls during the expected time of heat stress in Iran (June to September 2009). Control group (n=10) were fed a standard concentrate feed while the treatment group (n=9) had this feed top dressed with 100 g of an omega-3 enriched nutriceutical. Semen volume, sperm concentration and total sperm production were evaluated on ejaculates collected after 1, 5, 9 and 12 weeks of supplementation. Moreover, computer-assisted assessment of sperm motility, viability (eosin-nigrosin) and hypo-osmotic swelling test (HOST) were conducted. Results: Heat stress affected sperm quality parameters by weeks five and nine of the study (p<0.05). Supplementation significantly increased total motility, progressive motility, HOST-positive spermatozoa and average path velocity in the fresh semen of bulls (p<0.05). Conclusion: Dietary omega-3 supplementation improved in vitro quality and motility parameters of fresh semen in Holstein bulls. However, this effect was not evident in frozen-thawed semen.
Cell Journal, 2013
Objective: The effects of dietary fish oil on semen quality and sperm fatty acid (FA) profiles during consumption of n-3 FA as well as the persistency of FA in ram's sperm after removing dietary oil from the diet were investigated. Materials and Methods: Nine Zandi's rams were randomly assigned to two groups (isoenergetic and isonitrogenous diets): Control (CTR; n=5) and Fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the end week of feeding period (phase 1). After feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removing FO (phase 2). The sperm parameters and FA profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed using the SPSS 16. Does the conventional diet contain any fats/lipids? If so, what is the concentration of these fats given to the control group compared to treatement group? No, it dosnt. This diet typically have 2.5-3 % ether extract or crude fat originated from forages and some garin such as barley. Results: Dietary FO had significant positive effects on all sperm quality and quantity parameters compared with CTR during feeding period (P<0.05). The positive effects of FO on sperm concentration and total sperm output were observed one and two months after removing FO (P<0.05), whereas, other sperm parameters were unaffected. Before feeding, C14 (Myristic acid), C16 (Palmitic acid), C18 (Stearic acid), C18:1 (Oleic acid) and C22:6 Cell Journal (Yakhteh
Cell journal, 2014
The effects of dietary fish oil on semen quality and sperm fatty acid profiles during consumption of n-3 fatty acids as well as the persistency of fatty acids in ram's sperm after removing dietary oil from the diet were investigated. In this experimental study, we randomly assigned 9 Zandi rams to two groups (isoenergetic and isonitrogenous diets): control (CTR; n=5) and fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the last week of the feeding period (phase 1). After the feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removal of FO (phase 2). The sperm parameters and fatty acid profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed with SPSS version 16. Dietary FO had significant positive effects on all sperm quality a...
The goal of this study was to investigate the effect of fish oil–supplemented diet on fresh and post-thaw semen quality and sperm lipid composition in bulls. Bulls were randomly assigned to two groups (n ¼ 6). Six bulls were used as the control group and six received the fish oil (1.2% dry matter of total diet) for 11 weeks. Semen was individually collected from each bull and frozen biweekly. Semen volume, sperm concentration, viability, progressive motility, and fatty acid profile of sperm were measured in 1st, 3rd, 5th, 7th, 9th, and 11th week of experiment. Viability, progressive motility, and fatty acid profile of post-thaw sperm were also measured in 3rd, 5th, 9th, and 11th week of experiment. Data were analyzed with using Proc GLM or MIXED (for repeated measurement data) in SAS program. The fish oil–supplemented diet increased the semen volume and sperm concentration. The fish oil–supplemented diet also altered the viability, progressive motility, and fatty acid profile of fresh and post-thaw sperm. In conclusion, feeding a fish oil– enriched diet via alteration of fatty acid profile of sperm lipid could improve in vitro quality of fresh and post-thaw sperm in Holstein bulls.