Evaluation of the growth and sporulation of different entomopathogenic fungi in different liquid and solid media at varied concentrations (original) (raw)
Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides worldwide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.
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Mass production of entomopathogenic fungi using agricultural products and by products
Various agricultural products and by products such as grains, vegetable wastes, seeds, rice husk, saw dust and liquid media such as coconut water, rice and wheat washed water and rice cooked water were evaluated for mass production of three entomopathogenic fungi; Beauveria bassiana, (Bals.) Vuil. Paecilomyces fumosoroseus (Wize) Brown and Smith and Verticillium lecanii. (Zimm) Viegas. Among the grains, wheat supported maximum spore production for B. bassiana while sorghum recorded maximum spore production in P. fumosoroseus and V. lecanii. Similarly carrot, jack seeds and ladies finger also supported good growth and sporulation of all the three tested fungi. Coconut water supported maximum growth and sporulation.
Journal of Pure and Applied Microbiology, 2015
A systematic study was conducted to develop the cost effective entomopathogenic fungal biopesticide. The different grains like Sorghum, Ragi, Paddy and Rice were selected based on their local availability and their lower in cost. However the carbon and nitrogen requirement was met by adding 1 per cent yeast extract. Among all the treatments, the half crushed rice kernels used treatment showed their best by producing highest spore load of 2.15 spores g-1. This was followed by half crushed Sorghum and Maize. Rice kernels also retained the highest B:C ratio (7.91:1). All together it is concluded that Rice and Sorghum could be considered as the best source for mass production of entomopathogens.
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