Effect of Operating Conditions on Molasses fermentation for Bioethanol production (original) (raw)

Impact of Storage Time, Rain and Quality of Molasses in the Production of Bioethanol

Due to environmental concerns and the rising prices of petroleum products, usually bio-ethanol is being produced in greater amount from sugar cane molasses in Pakistan. In this work various properties related to molasses are being analyzed by performing experiments at the Distillery Plant, AASML (Al-Abbas Sugar Mills Limited), Mirpurkhas, Sindh, Pakistan. Brix, Sucrose, pH, ethyl alcohol, RS (Reducing Sugar), TSI (Total Sugar as an Invert), TS (Total Sugar), purity and presence of micro-organisms are

Production of Ethanol From Sugar Cane Molasses and Evaluation of Its Quality

The objective of the present study was to produce ethanol from final sugar cane molasses and to evaluate its quality. Final cane molasses samples were obtained from Elguneid Sugar Factory. Urea was added in different concentrations (0.15%, 0.5%, and 0.25%) and used as nitrogen source to determine optimum concentration. Experiments were conducted using four treatments depending upon molasses sugar concentration which was calculated as percentages (10, 15, 20 and 25). The pH of the mash was adjusted to 4.8 using conc. sulphuric acid. 5% baker's yeast (DCL) was added. The fermentation was conducted for 72 hours, at 33ْ C. The microbiological analysis revealed absence of bacteria, yeasts and moulds in dilutions 10 -3 , 10 -4 , 10 -5 of molasses samples. The yield ethanol obtained was 20 ml per 100 gm of molasses, and the maximum concentration of ethanol (96%) could be obtained when the main medium of production) molasses) includes 0.25% urea and 20% sugar concentration.

Production of Ethanol from Sugarcane Molasses

International Journal For Research in Applied Sciences and Biotechnology

Ethanol is a potential energy source and its production from renewable biomass has gained lot of popularity. There has been worldwide research to produce ethanol from regional inexpensive substrates. The present study deals with the optimization of process parameters (viz Ph, Substrate conc, Urea conc) for ethanol production from sugar cane molasses. Sugar cane molasses are cheapest source of ethanol production it can also produce from wheat, sugar beet and corn etc. The study was carried out by process of parameter optimization. The process parameters optimized were substrate conc, pH and urea conc. The values of the process parameters are 30% substrate conc, pH 4.5 and urea conc 0.5%. Fermentation period was 7 to 8 days at 28°C.

Bioethanol production from sugarcane molasses by instant dry yeast (effect of pretreatment and fermentation temperature)

IOP Conference Series: Earth and Environmental Science

Some bioethanol industries in Indonesia that use sugarcane molasses as raw material often face low level of ethanol content from fermentation process. The molasses condition and the temperature fluctuation are supposed to be the cause. This research aimed to determine the effect of sugarcane molasses pre-treatment with H 2 SO 4 and fermentation temperature in bioethanol production by using instant dry yeast of Saccharomyces cerevisiae. Factorial Randomized Block Design with 2 factors was used in the research. The first factor was pre-treatment effect in sugarcane molasses (with and without pre-treatment) and the second was fermentation temperature (29 0 C, 32 0 C and 35 0 C). The data were analysed using analysis of variance or ANOVA and continued with further test Duncan Multiple Range Test or HSD (Honestly Significant Difference) with confidence interval of 5%. The best treatment was determined using Multiple Attribute Test. Results showed that sugarcane molasses pretreatment with H 2 SO 4 and fermentation temperature had a significant effect (α= 0.05) on bioethanol production. The best result was obtained from sugarcane molasses medium that given H 2 SO 4 pre-treatment and fermentation temperature of 32 0 C with total decrease value of total soluble solids (% brix) of 10.9 % brix, total decrease sugar of 12.15%, reducing sugar consumption of 57.21 g/L, ethanol content of 8.30 % and yield ethanol of 68.67 %.

Enzymatic hydrolysis of sugarcane molasses as pre-treatment for bioethanol production

International Sugar Journal

This study aimed at analysing the influence of enzymatic pre-hydrolysis of sugarcane molasses with α-amylase and amyloglucosidase on the subsequent alcoholic fermentation, with the objective of increasing bioethanol productivity. Sugarcane molasses must was treated with the amylolytic enzymes and then submitted to alcoholic fermentation for 8 h at 32 o C, in presence of baker's yeast (Saccharomyces cerevisiae) and nutrients. Analyses carried out for sugars and ethanol yield showed the efficiency of the pre-treatment, concerning the increase in fermentable sugars. The best result for the treatment of molasses with the two enzymes yielded a 10.2% increase of sugars in comparison to molasses without pre-treatment. However, there was greater ethanol yield for sugarcane molasses must treated only with the α-amylase enzyme, which gave an average ethanol yield of 98% and productivity of 12.5 g L-1 h-1 , considered satisfactory.

Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast

Microbiology and Biotechnology Letters

Bioethanol has recently attracted much attention as a sustainable and environmentally friendly alternative energy source. This study aimed to develop a potential process for bioethanol production by fed-batch fermentation using instant dry yeast. To obtain the highest cell growth, we studied the influence of the initial sugar concentrations and pH of sugarcane molasses in batch fermentation. The batch system employed three levels of sugar concentrations, viz. 10%, 15%, 20% (w/v), and two levels of pH, 5.0 and 5.5. The highest cell growth was achieved at 20% (w/v) and pH 5.5 of molasses. The fed-batch system was then performed using the best batch fermentation conditions, with a molasses concentration of 13% (w/v) which resulted in high ethanol concentration and fermentation efficiency of 15.96% and 89%, respectively.

Bioethanol production from molasses by different strains of Saccharomyces cerevisiae

In commercial ethanol production producers often use sugar cane molasses as raw material due to their abundance and low costs. The most employed microorganisms used for fermentation is Saccharomyces cerevisiae yeasts due to their ability to hydrolyze sucrose from cane molasses into glucose and fructose, two easily assimilable hexoses. The aim of this study was to evaluate the application of different strains of Saccharomyces cerevisiae for sugar cane molasses in order to produce bioethanol. According to the obtained results the strain D1 (Safdistil C-70) achieved higher values of the specific growth rate in comparison with other strains used. The maximum ethanol productivity of 2.33 g/L . h was achieved around 36 hours of fermentation by using the yeast D1. Therefore, the optimal duration of the fermentation process in technical and economic terms should be considered.

Bioethanol production from sugarcane molasses by instant dry yeast

IOP conference series, 2019

Sugarcane molasses is a by-product of sugarcane products that can be used as bioethanol production. Bioethanol is ethanol produced through a fermentation process that can be done by various microorganisms such as Saccharomyces cerevisiae. Commercial instant dry yeast on this fermentation was chosen because it can be directly used as a starter to simplify the production process and reduce the bacterial contamination risk. The sugar industry in West Java is one of the sugarcane processing industries that produce molasses in Indonesia. Then molasses were processed into bioethanol. However, the ethanol content on bioethanol production in this industry is low (± 4-6%). Some factors can cause low ethanol production such as the presence of mineral contents and sugar concentrations in molasses. The aim of this study is to investigate the effect of H 2 SO 4 pretreatment and sugar concentration on ethanol production. Three level of sugar concentration i.e. 20%, 25% and 30% Brix were compared for ethanol production using with and without pretreated molasses. The obtained data were analyzed statistically using Analysis of Variance (ANOVA). Under the best condition (30% sugar concentration with H 2 SO 4 pretreatment), the ethanol concentration could be increased by 3.5-5.5%.

Bioethanol Production from Sugarcane Molasses with Simultaneous Saccharification and Fermentation (SSF) Method using Saccaromyces cerevisiae-Pichia stipitis Consortium

IOP Conference Series: Earth and Environmental Science, 2018

The sugar-making industry from sugar cane has a byproduct of sugar cane as molasses. One alternative to reduce the amount of molasses waste is by reusing molasses as biomass for bioethanol production. Molasses can be reused as bioethanol that have economic value because of their high sugar content. The process of making bioethanol from molasses is done by Simultaneous Saccharification and Fermentation (SSF) method using 10% substrate (100 gr / L molasses) and 20% substrat (200 gr / L molasses). The process of hydrolysis of sugarcane molasses is carried out biologically using Aspergillus niger and Trichoderma viride, while the fermentation process uses the consortium Saccaromyces cerevisiae-Pichia stipitis. Ethanol production using the SSF methods had the highest bioethanol concentration of 14.38% v/v for 20% substrat and 10% consortium usage at 72 hours fermentation.

Experimental Synthesis of Ethanol from Sugar Cane Juice Molasses

http://www.gijash.com/archive\_gijash\_vol.1\_issue1.html, 2017

Ethanol synthesis by using raw feed stock is being widely explored. Production of ethanol from waste food grains, fruit wastes, vegetable wastes, and sugar industry byproducts is being carried out on experimental, pilot plant and in some cases industrial scale. Ethanol has huge potential as fuel and is main compound in alcoholic beverages. It is most often used as a motor fuel, mainly as a bio fuel additive for gasoline. Bio ethanol can be made from very common crops such as sugar cane, potato, manioc and corn. In current research sugar molasses is used for ethanol synthesis. Key words:Bacteria, molasses, purity, incubation, slant, fermentation.