Histometric analysis of alveolar bone regeneration with expanded polytetrafluoroethylene (e-PTFE) and latex membranes (original) (raw)
Related papers
Materials
Aim: The aim of the present study was to investigate quantitative histological examination of bone reconstructed with non-resorbable high-density polytetrafluoroethylene membrane (d-PTFE), left intentionally exposed in post extraction sockets grafted with anorganic bone material, and removed after four weeks, versus extraction and guided bone regeneration (GBR), performed two months later. Materials and Methods: This study was designed as a multicenter randomized controlled trial of parallel-group design. Patients were selected and consecutively treated in three centers in Italy. Patients randomly received intentionally exposed non-resorbable d-PTFE membrane (group A), or guided bone regeneration (group B), to treat post-extractive alveolar bone defects with implant-supported restorations. Outcomes were: the implant failure, any mechanical and biological complications, patient satisfaction, and qualitative and histomorphometric evaluation of the collected bone samples. Results: Eigh...
Acta Veterinaria, 2020
Post extraction alveolar ridge preservation is a preventive oral surgical procedure aimed to preserve the dimensions of the alveolar ridge after removal of the teeth. According to literature data, the utilization of guided bone regeneration for this purpose is absolutely justified. The aim of this study was to examine the effect of resorbable collagen membranes placed in two layers in respect to the application of membranes placed in one layer to the degree of bone regeneration after a tooth extraction. This experimental study was conducted on six adult dogs. In the first phase all four premolars were extracted on both sides in the lower jaw. After the volume-standardisation with bone drill, three post extraction alveoli were filled with bone substitute of equine origin and then covered with: collagen bioresor bable membrane of porcine origin; collagen bioresorbable membrane of equine origin; pericardial bioresorbable membrane of equine origin. The membranes on the left side were pl...
Journal of Materials Science-Materials in Medicine, 2014
This work assessed the performance of membranes made of natural latex extracted from Hevea brasiliensis prepared with three different methods: polymerized immediately after collection without the use of ammonia (L1); polymerized after preservation in ammonia solution (L2); and polymerized after storage in ammonia, followed by Soxhlet technique for the extraction of substances (L3). Polytetrafluoroethylene (PTFE) membrane was used as control. Two 10-mm diameter bone defects were surgically made in the calvaria of thirty adult male New Zealand rabbits. Defects (total n = 60) were treated with guided bone regeneration (GBR) using L1, L2, L3 or PTFE membranes (n = 15 for each membrane). Ten animals were euthanized after 7, 20 and 60 days postoperatively so that five samples (n = 5) of each treatment were collected at each time, and bone regeneration was assessed microscopically. The microscopic analysis revealed defects filled with blood clot and new bone formation at the margins of the defect in all 7-day samples, while 20-day defects were mainly filled with fibrous connective tissue. After 60 days defects covered with L1 membranes showed a significantly larger bone formation area in comparison to the other groups (P \ 0.05, ANOVA, Tukey). Additionally, bone tissue hypersensitization for L1 and PTFE membranes was also investigated in six additional rabbits. The animals were subjected to the same surgical procedure for the confection of one 10-mm diameter bone defect that was treated with L1 (n = 3) or PTFE (n = 3).
Guided bone regeneration with intentionally exposed membranes and its implications for implant d
Journal of Osseointegration, 2010
Aim Post-extraction alteration of the alveolar bone topography is one of the main problems associated with tooth loss. Guided bone regeneration (GBR) technique avoids in great part alveolar bone resorption after extractions This randomized controlled study aimed to evaluate the influence of early membrane exposure on GBR in humans. Materials and methods The study involved 13 patients, each with 2 premolars with orthodontic indication for extraction. After extractions, clinical measurements were recorded for alveolar depth (AD), buccal-lingual (BL) and mesio-distal (MD) dimensions of the alveoli, and radiographic measurements for radiographic alveolar depth (RAD), width (AW), and total alveoli area (AA). Polytetrafluorethylene (e-PTFE) membranes were then adapted and fixed over the extraction sockets, and based on membrane coverage sockets were divided in: Group I - flap was rotated to completely cover the membrane; Group II - flap was conventionally replaced and membrane remained, i...
Journal of Periodontology, 2009
Background: Remodeling and resorption of the alveolar crest, specifically at the buccal aspect, characterize the healing extraction socket. These result in narrowing and shortening of the alveolar ridge, which compromise esthetics and complicate restoration. Alveolar ridge augmentation has been proposed to facilitate future site restoration by minimizing ridge resorption. Therefore, the purpose of this study was to compare extraction socket healing and alveolar ridge alteration after socket augmentation using bone allograft covered with an acellular dermal matrix (ADM) or polytetrafluoroethylene (PTFE) membrane.
Guided bone regeneration using resorbable and non-resorbable membranes: a histological study in dogs
Odonto-stomatologie tropicale = Tropical dental journal, 2012
The aim of this study was to evaluate the effectiveness of e-PTFE titanium reinforced and Guidor membranes in the management of alveolar ridge deformities. Five beagle dogs were included in the study. Three through and through defects of the same size (12 x 5 x 5) were created in each animal, one on the right side and two defects 3-4 mm distant from each other on the left side. Guidor membrane was applied to the right defect, e-PTFE was applied to one of the left defects while the third defect served as control. Three, six and nine months following membrane placement, the dogs were euthanized and membranes with surrounding bone tissues harvested for histological analysis. Healing in each animal was uneventful with no clinical sign of inflammation. Histological evaluation indicated the defects covered with both membranes had significantly gained greater bone formation than the control defects. On the other hand, the control defects healed thin rim of bone mostly with a persistent cen...
Effects of latex membrane on guided regeneration of long bones
Journal of Biomaterials Science, Polymer Edition, 2019
Effects of latex membrane in guided regeneration of long bones Natural latex extracted from Hevea brasiliensis is one of the materials pointed out as potential tissue regenerators. The use of latex-based membranes in bone regeneration might be an alternative to stimulate bone formation. The aim of this study was to evaluate the effects of latex membranes in guided bone regeneration of defects produced in long bones of rats. Sixty rats were equally divided into latex and control groups, and each group was subdivided into two subgroups according to treatment duration of one and four weeks. Bone defects with 2.5 mm in diameter were surgically made in the left tibia. In the animals of the latex group, a latex membrane was placed over the bone defect. The samples underwent quantitative histological analysis of bone formation and collagen matrix, immunohistochemical analysis of osteogenic protein markers, assessment of bone mechanical properties and bone densitometry, and radiological assessment. The osteocalcin immunostaining data were submitted to the generalized linear model test with two independent factors. For the other data, the multivariate ANOVA with two independent factors was performed. The use of the latex membrane significantly improved (p<0.005) the volume of newly formed bone, collagen type I matrix, expression of osteopontin, and bone stiffness, both in the early and late stages of regeneration. In conclusion, the latex membrane was able to promote bone regeneration in long bones.