Interaction of Desulfovibrio desulfuricans biofilms with stainless steel surface and its impact on bacterial metabolism (original) (raw)
Related papers
The influence of surface material on the development of Desulfovibrio desulfuricans biofilms
Sulphate reducing bacteria have an important role in the sulphur cycle, and therefore in wastewater treatment systems. They are able to form biofilms on metallic surfaces, leading to fouling and corrosion problems. Additionally, hydrogen sulphide that is a product of their metabolism can cause serious health risks. In this study, sulphate reducing bacteria (SRB) biofilms were developed on stainless steel 304 and on polycarbonate in order to evaluate surface effect on biofilm formation. Results showed that the biofilm formed on stainless steel presented higher metabolic activity, confirmed by lactate and sulfate removals. Metal elements present in stainless steel may affect SRB activity. This can be the case of nickel that represents around 8% of stainless steel 304. Studies performed with suspended cultures of Desulfovibrio desulfuricans also showed that the presence of nickel in the media had a positive impact on bacterial activity.
The influence of nickel on the adhesion ability of Desulfovibrio desulfuricans
Colloids and Surfaces B: Biointerfaces, 2005
The build-up of biofilms on metals surfaces may lead to severe corrosion, especially in the presence of sulphate-reducing bacteria (SRB). To prevent the deterioration of material caused by biofilms it is necessary to understand the processes governing biofilm development including mechanisms of cell adhesion. Additionally, corrosion of metallic surfaces due to bacteria may lead to the dissolution of metallic elements that may further affect adhesion and biofilm development. A study was carried out to evaluate how the presence of nickel in the substrata affects the adhesion ability of Desulfovibrio desulfuricans. The substrata tested were stainless steel 304 (SS), metallic nickel (Ni) and polymethylmetacrylate (PMMA), a non-metallic material used as control. The influence of nickel on SRB growth and its relation to adhesion was also checked. A statistically significant difference in the number of adhered cells to the materials tested was detected, with higher bacterial number on nickel, followed by SS and finally by PMMA. The higher number of SRB adhered to steel compared with PMMA may be explained by differences in hydrophobicity, in roughness and in the electron-acceptor character of the substrata. Additionally, bacterial growth was found to be positively affected by the presence of nickel as revealed by a significant increase in the specific growth rate of SRB in the presence of increased nickel concentrations.
Effect of biofilm in the corrosion of austenitic stainless steels in wastewater treatment plants
The hydrogenotrophic sulfate-reducing bacterium (SRB) Desulfovibrio capillatus (DSM14982 T ) was isolated from an oil field separator with serious corrosion problems; this is the study of its role in the corrosion of carbon steels under anaerobic conditions. Immersion tests with two steel alloys, St-35.8 (typical carbon steel employed in European naval industry), and API-5XL52 (weathering alloy steel employed in Mexican oil industries) were performed. Total exposure was 45 days and different concentrations of thiosulfate as electron acceptor for bacterial growth were employed. The samples immersed in media with SRB undergo fast activation and numerous active sites form on the surface. Microscopic observations were made by environmental scanning electron microscopy (ESEM). Weight loss and electrochemical testing included open circuit potential (E corr ), polarization resistance (R p ), electrochemical impedance spectroscopy (EIS) and electrochemical noise (EN) were measured with and without bacteria in the culture medium in order to determine corrosion rates and 0010-938X/$ -see front matter Ó mechanisms. All electrochemical techniques have shown that after the end of the exponential phase the corrosion activity notably increased due to the high concentration of bacterial metabolites. Finally, the corrosion behavior of API-5XL52 was worse than St-35.8.
Materials and Corrosion, 2017
The paper presents the results on biofilm formation induced by pure Desulfovibrio desulfuricans strains and its effect on corrosion behaviour of 2205 duplex stainless steel (DSS). Biofilm formation and damage process stimulated by D. desulfuricans strains are analysed with reference to their metabolic activity and to the surface characterisation of structure and configuration of the biofilm. Electrochemical techniques are applied to monitor bacterial attachment to the steel surface and to determine the influence of bacteria on the passivity and corrosion resistance of 2205 DSS. The obtained results prove that 2205 DSS is rapidly colonised by D. desulfuricans and that the biofilms formed affect its corrosion resistance. Bacterial activity leads to the more noble corrosion potential values and to the increased current densities on the potentiodynamic polarisation curves. SEM observations reveal micropits as well as signs of crevice attack on the steel surface.
Water Science and Technology, 2020
Ag-Cu ions in cooling water may inhibit the activity of sulfate-reducing bacteria and therefore provide solutions to microbiologically induced corrosion (MIC) problems, mainly caused by Desulfovibrio sp. To investigate this, the MIC behavior of Desulfovibrio sp. on 316L stainless steel in terms of growth and extracellular polymeric substances (EPS) production was investigated in the presence of Ag-Cu ions. Laboratory-scale systems were set up with final concentrations of 0.13 ppm Ag and 0.3 ppm Cu ions, as they are the frequently used doses for cooling waters, and operated over 720 hours. The corrosion rate was evaluated by gravimetric assay, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analyses. The growth of Desulfovibrio sp. was assessed by bacterial counting and EPS production. Ag-Cu ions in the biofilm were assessed by inductively coupled plasma - optical emission spectrometry (ICP-OES) and EDS-elemental mapping analyses. It was concluded that the...
International Biodeterioration & Biodegradation
The microbiologically influenced corrosion (MIC) of zinc and galvanized steel caused by a sulfate reducing bacterium (SRB) was investigated. After 7 days of incubation of Desulfovibrio vulgaris in 125 mL anaerobic vials (100 mL culture medium) at 37 • C, the sessile cell coverage on the galvanized steel was slightly higher than that on pure zinc: (1.9 ± 0.2) × 10 9 cells/cm 2 vs. (9.0 ± 1.8) × 10 8 cells/cm 2. The weight losses for galvanized steel and pure zinc were 31.5 ± 2.5 mg/cm 2 and 35.4 ± 4.5 mg/cm 2 , respectively, which were 10 1 higher than that for carbon steel. The corrosion current densities of galvanized and pure zinc were 25.5 μA/cm 2 and 100 μA/cm 2 , respectively after the 7-day incubation, confirming that galvanized steel was less prone to SRB MIC despite having a slightly higher sessile cell count. In both cases, the corrosion product was mainly ZnS. Three MIC mechanisms were possible for the severe corrosion against the two metals. Extracellular electron transfer MIC (EET-MIC) was thermodynamically favorable for zinc. Furthermore, in the presence of Zn coupons, H 2 evolution in the headspace was 5.5 times higher than without Zn coupons, which suggested that proton attack and/or H 2 S attack also occurred in the corrosion process.
Influence of Desulfovibrio sp. biofilm on SAE 1018 carbon steel corrosion in synthetic marine medium
Corrosion Science, 2007
This work assessed the effect of an enriched culture medium and synthetic seawater on the growth and production of exopolymeric substances (EPS) of a Desulfovibrio sp. strain, isolated from a Mexican oil well. The EPS (mainly consisting of proteins) growth was only achieved after exposing sulfate-reducing bacteria to culture media under dissimilative conditions that predominantly promoted the growth of the biofilm and a small concentration of microorganisms. Once this EPS film was obtained, the evolution of SAE 1018 carbon steel/biofilm/synthetic seawater (VNNS medium) interface was further studied using electrochemical impedance spectroscopy technique (EIS). This study revealed strong adhesion of the biofilm during the formation of iron sulfide (pirrotite) on carbon steel surface. The biofilm inhibits the accelerated damage of the steel for some time exhibiting impedance values of 30 000 X. However, at longer times the chemical environment around the biofilm, as a result of microbial metabolism, may become quite corrosive to steel.
Desulfovibrio Vulgaris, a species of Sulphate Reducing Bacteria (SRB) that are mostly found living in the absence of oxygen or namely anaerobic condition is an imminent threat to pipeline long-term integrity. Pipeline infrastructure may experience severe metal loss due to corrosion induced by the species and can potentially lead to catastrophic failure. Desulfovibrio Vulgaris (ATCC7757) was cultured in broth number 1249 (Modified Barr's Medium) to study the effect of bacteria growth upon metal loss. The medium was modified to pH 7.0 at 37°C. Carbon steel coupons grade X70 were cut to approximately 10mmx20mmx5mm and kept in anaerobic vials. After 28 days of incubation, samples were retrieved to determine the weight loss of the carbon steel. Based on the weight loss graph pattern, the amount of weight loss significantly depends on the exposure time to the bacteria. The weight lost dramatically increases from day-1 to day-28 to signify the common pattern of corrosion induced by SRB.
The Influence of the Desulfovibrio desulfuricans 14 ATCC 27774 on the corrosion of mild steel
Materials and Corrosion, 2000
The involvement of sulphate-reducing bacteria (SRB) in microbially influenced corrosion (MIC) of steel and the serious implications associated with their presence in industrial environments have long been known and extensively described. Desulfovibrio desulfuricans ATCC 27774 is an interesting metabolic case of SRB, as it can use both sulphate and nitrate as respiratory substrates during lactate oxidation. This strain has been extensively studied from both a biochemical and structural point of view but, so far, restricted information is available concerning its role in MIC. This work describes a comparative study of the corrosive aggressivity of ATCC 27774 strain towards mild steel when grown either in lactate/sulphate or lactate/nitrate media. The carbon source and electron acceptor's consumption rates were analysed and the metabolic features were correlated with weight loss measurements and SEM observations. Die Beteiligung von sulfatreduzierenden Bakterien (SRB) bei der mikrobiologisch beeinfluûten Korrosion (MIC) von Stahl und die Auswirkungen, die mit ihrer Anwesenheit in industriellen Umgebungen verbunden sind, sind seit langem bekannt und ausfu Èhrlich beschrieben. Desulfovibrio desulfuricans ATCC 27774 ist ein interessanter metabolischer Fall von SRB, da es sowohl Sulfat als auch Nitrat als Respirationssubstrat wa Èhrend der Laktatoxidation nutzen kann. Diese Art ist sowohl vom biochemischen als auch vom strukturellen Standpunkt aus intensiv untersucht worden; bisher gibt es allerdings nur begrenzte Informationen u Èber seine Rolle bei MIC. Diese Arbeit beschreibt eine Vergleichsstudie der Korrosivita Èt der ATCC 27774 Art (bei Wachstum entweder in Laktat/Sulfatoder Laktat/Nitrat-Medien) gegenu Èber unlegiertem Stahl. Die Kohlenstoffquelle und die Elektronenakzeptorverbrauchsrate wurden analysiert und die metabolischen Merkmale wurden mit Massenverlustmessungen und REM-Beobachtungen korreliert. 2 Materials and methods 2.1 Microorganisms , growth media and culture conditions Studies were performed with pure cultures of the strain Desulfovibrio desulfuricans ATCC 27774, grown in lactate/sulphate and lactate/nitrate media whose composition is described below.
Corrosion behavior of low-alloy steel in the presence of Desulfotomaculum sp.
Corrosion Science, 2009
The objective of this study was to determine the effect of sulfate-reducing Desulfotomaculum sp. bacteria isolated from a crude oil field on the corrosion of low-alloy steel. The corrosion rate and mechanism were determined with the use of Tafel slopes, mass loss method and electrochemical impedance spectroscopy (EIS). The formation of the biofilm and the corrosion products on the steel surface was determined with scanning electron microscopy (SEM) micrographs and energy dispersive X-ray spectra (EDS) analysis. It was observed from the Tafel plots that the corrosion potential exhibited a cathodic shift that verifies an increase in the corrosion rates. The semicircles tended to open at lower frequencies in the Nyquist plots which indicates the rupture of the protective film. The corrosion current density reached its maximum value at the 14th hour after the inoculation and decreased afterwards. This was attributed to the accumulation of corrosion products on the surface.