Recovery and Molecular characterization of poly-β-hydoxybutyrate polymer by Bacillus cereus isolate P83 (original) (raw)
Related papers
CHARACTERIZATION OF POLYHYDROXYBUTYRATE SYNTHESIZED BY BACILLUS CEREUS Original Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objectives: To characterize the Polyhydroxybutyrate (PHB) produced from Bacillus cereus using various instrumental methods and reduce them to the nanoscale which can be used as drug carrier Methods: The isolated bacterium was identified by 16S rDNA analysis. PHB produced by the bacterium was extracted by a process using boiling chloroform followed by methanol which selectively precipitates PHA. The polymer produced was analyzed using various instrumental techniques like FTIR, GC-MS, 1 Hand 13 Results: A bacterium with the ability to produced PHB was isolated from the cloth used to smear oil on pan cake pan which was identified as Bacillus cereus by 16S rDNA analysis. The organism was capable of accumulating 1.19 g L C NMR, XRD and FESEM. This biogenic PHB has been reduced to nanospheres which were analyzed by FESEM.-1 Conclusion: The organism seems to be a potential candidate for the biogenic synthesis of PHB which can find application as drug carrier. of PHA corresponding to 49.7% of its dry weight after 48 h of incubation. The polymer produced was analyzed using various instrumental techniques which identified the polymer as PHB.
Production of Poly-β-hydroxybutyric acid (PHB) by Bacillus cereus
International Journal of Current Microbiology and Applied Sciences, 2016
One poly-β-hydroxybutyrate producing bacterial from different microbial sources was isolated and characterized for their morphological, biochemical properties. Based on their 16S rDNA, it was identified as Bacillus cereus E6. The bacterial strain was screened for PHB production and compared for the intensity of fluorescence using Nile red staining method. PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with sucrose and ammonium sulphate by B. cereus, respectively. Regarding incubation time as well as temperature and pH, optimum PHB production conditions were 48 h, 30 °C and 7, respectively. B. cereus is capable of accumulating appreciable levels of PHB from glucose, xylose, lactose, whey, molasses, sugar cane bagasse, rice straw hydroysate when 2% from all substrates were used an alternative carbon for the PHB production. Ammonium sulphate was the best nitrogen source. C/N ratio was also one of the factors that affected the production of PHB. The ratio of C/N that reaches 20:1 was considered the best ratio to produce the highest production of PHB. The present study provide the useful data about the optimized conditions for PHB production by B. cereus that can be utilized for industrial production of PHB, a fast emerging alternative of nonbiodegradable plastics.
Polymers, 2022
In this work, the strains Bacillus megaterium BM 1, Azotobacter chrocococcumAz 3, Bacillus araybhattay RA 5 were used as an effective producer of poly-3-hydroxybutyrate P(3HB). The purpose of the study was to isolate and obtain an effective producer of P(3HB) isolated from regional chestnut soils of northern Kazakhstan. This study demonstrates the possibility of combining the protective system of cells to physical stress as a way to optimize the synthesis of PHA by strains. Molecular identification of strains and amplification of the phbC gene, transmission electron microscope (TEM), extracted and dried PHB were subjected to Fourier infrared transmission spectroscopy (FTIR). The melting point of the isolated P(3HB) was determined. The optimal concentration of bean broth for the synthesis of P(3HB) for the modified type of Bacillus megaterium RAZ 3 was 20 g/L, at which the dry weight of cells was 25.7 g/L−1 and P(3HB) yield of 13.83 g/L−1, while the percentage yield of P(3HB) was 53....
Archives of Pharmaceutical Sciences Ain Shams University
Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrientlimited conditions. However, their commercialization is hindered by production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83. Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1. In conclusion, B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process.
Biosynthesis of Medium Chain Length Poly-β-hydroxybutyrate by Pseudomonas aeruginosa Dw7
Polyhydroxya lkanoates (PHAs), are a promising family of bio-based polymers, which considered to be alternatives to traditional petroleum-based plastics. Poly-β-hydroxybutyrate (PHB) is the most known degradable biopolymers, produced by bacterial genera. It is generally accepted that PHB can be used instead of plastic to solve one of the greatest problems facing the environment. Pseudomonas aeruginosa Dw7 was grown on mineral salt medium supplemented with waste cooking of corn oil for the synthesis of a medium chain-PHB. This study is divided into three steps, at first fermentation of P.seudomonas aeruginosa Dw7, which was carried out aerobically at optimum temperature of 30˚C and 500 rpm of agitation speed. Lab scale bioreactor (5L) operated as a batch culture system for 80 hours. The highest cell dry weight (CDW) of bacteria reached to 5.3 g/l observed after 60 h of operation, which was corresponding to 62.6 % of PHB. The results suggested the efficiency of the system for production of PHB in large scale. Many solvent systems were conducted to evaluate the best solvent for PHB extraction from bacterial cell in the second step. Chloroform– hypochlorite dispersion extraction was followed by that extraction with chloroform showed the best solvent system for yielding of PHB. Relatively; it has the high rate with 63% yield of PHB. Characterization study of PHB was the last step. PHB were included chemical, physical; mechanical and solubility properties of the produced PHB have been characterized by many analyses techniques, comprising Gas Chromatography Mass Spectrometry (GC-MS), Fourier-transform Infrared Spectroscopy (FTIR), X-Ray diffraction (X-Ray) and melting point. PHB was a yellowish white crystal, soluble in chloroform and other chlorinated hydrocarbons like dichloromethane, dichlorethane and chloropropane. X-Ray diffraction (XRD) study was carried out to check if the polymer had a crystalline or amorphous structure. The increased intensity of peaks showed that the polymer had more organized packed crystalline structure. The results of the GC-MS recorded that there were 9 different active peaks predictive. The two major compounds that were produced by P. aeruginosa Dw7 were then identified as undecanoic acid which eluted at 15.4 min and tridecanoic acid at 17.3 min with relative abundance of 100% and 33.14% respectively. The characteristic FTIR peaks for PHB indicated that the most prominent marker band for the identification of PHB is the ester carbonyl band at C=O, OH and CO .
BMC Microbiology, 2022
Background: Polyhydroxybutyrate (PHB) is a biopolymer formed by some microbes in response to excess carbon sources or essential nutrient depletion. PHBs are entirely biodegradable into CO 2 and H 2 O under aerobic and anaerobic conditions. It has several applications in various fields such as medicine, pharmacy, agriculture, and food packaging due to its biocompatibility and nontoxicity nature. Result: In the present study, PHB-producing bacterium was isolated from the Dirout channel at Assiut Governorate. This isolate was characterized phenotypically and genetically as Bacillus cereus SH-02 (OM992297). According to oneway ANOVA test, the maximum PHB content was observed after 72 h of incubation at 35 °C using glucose and peptone as carbon and nitrogen source. Response surface methodology (RSM) was used to study the interactive effects of glucose concentration, peptone concentration, and pH on PHB production. This result proved that all variables have a significant effect on PHB production either independently or in the interaction with each other. The optimized medium conditions with the constraint to maximize PHB content and concentration were 22.315 g/L glucose, and 15.625 g/L peptone at pH 7.048. The maximum PHB content and concentration were 3100.799 mg/L and 28.799% which was close to the actual value (3051 mg/l and 28.7%). The polymer was identified as PHB using FTIR, NMR, and mass spectrometry. FT-IR analysis showed a strong band at 1724 cm − 1 which attributed to the ester group's carbonyl while NMR analysis has different peaks at 169.15, 67.6, 40.77, and 19.75 ppm that were corresponding to carbonyl, methine, methylene, and methyl resonance. Mass spectroscopy exhibited molecular weight for methyl 3-hydroxybutyric acid. Conclusion: PHB-producing strain was identified as Bacillus cereus SH-02 (OM992297). Under optimum conditions from RSM analysis, the maximum PHB content and concentration of this strain can reach (3100.799 mg/L and 28.799%); respectively. FTIR, NMR, and Mass spectrometry were used to confirm the polymer as PHB. Our results demonstrated that optimization using RSM is one of the strategies used for reducing the production cost. RSM can determine the optimal factors to produce the polymer in a better way and in a larger quantity without consuming time.
Study of Polyhydroxybutyrate producing Bacillussp. isolated from Soil
2015
Poly-ß-hydroxybutyrate (PHB), one of the polyhydroxyalkanoates is the most popularly used bioplastic. These, being biodegradable and biocompatible polymers, are accumulated as energy reserve granules by many microbia intracellularly under carbon rich and nutrient starving conditions. PHB has proved itself as a promising alternative to non degradable plastics. PHB as bioplastics serves advantage of biological origin and can be completely degraded by variety of microorganisms. In the present study, different PHB producing microorganisms were isolated from soil using E2 medium and its rapid screening for PHB production was performed by Sudan Black B dye plate assay. The PHB accumulators were then subjected to spectrophotometric quantitation method and the highest accumulator was identified and characterized. Isolate KOL IV showed maximum accumulation of PHB and was optimized for its ability to accumulate maximum amount of PHB. It was identified as one of the Bacillus species and could ...
Production of Polyhydroxybutyrate Using Bacterial Strains
2015
Polyhydroxybutyrate is a polyhydroxyalkanoate (PHA), a polymer having a place with the polyesters classes. That areinterest as biodegradable and bio-derived plastics. The poly-3-hydroxybutyrate (P3HB) sort of PHB ispresumably the most widely recognized sort of polyhydroxyalkanoate, yet different polymers of this class are created by a mixture of life forms: these incorporate poly-4-hydroxybutyrate (P4HB), polyhydroxyvalerate (PHV), polyhydroxyhexanoate (PHH), polyhydroxyoctanoate (PHO) and their copolymers.Synbiotic sachets were obtained from general medical store and Soil was gathered from garden and screened for PHB delivering microscopic organisms. A few unidentified bacterial colonies were confined utilizing serial dilation method. Every bacterial colony was kept up in slants and liquid cultures. A lab strain of Lactobacillus was refined and kinetic studies were performed. Lactobacillus acidophilus produced PHB.
Optimization of Poly-B-Hydroxybutyrate Production from Bacillus species
2011
The amount of plastic waste increases every year and exact time for its degradation is unknown. Out of a total of 300 isolated strains one strain was selected for PHB production in different conditions like carbon source, nitrogen source and incubation temperature and time. When PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with raffinose and peptone. Regarding incubation time and temperature and pH, optimum PHB production conditions were 72h, 30°C and 7.0, respectively. The isolate was characterised biochemically as Bacillus species. The present study provide the useful data about the optimized conditions for PHB production by Bacillus species that can be utilized for industrial production of PHB, a fast emerging alternative of non biodegradable plastics.