Molecular cloning and deduced primary structures of acidic and basic phospholipases a2 from the venom of Deinagkistrodon acutus (original) (raw)

This study focused on analyzing the primary structures of acidic and basic phospholipases A2 (PLA2) found in the venom of the snake Deinagkistrodon acutus, aiming to understand their phylogenetic relationships. Using molecular cloning and PCR techniques, cDNA sequences were obtained, revealing strong similarities among the PLA2s across different species. Results suggest independent evolutionary paths for acidic and basic PLA2s within pit viper venoms, indicating a need for further sequence analysis to clarify interspecies relationships.