ROOTING OF ROOT CUTTINGS: A BREAKTHROUGH IN VEGETATIVE PROPAGATION (original) (raw)
Related papers
Effect of IBA (Indole Butyric Acid) levels on the growth and rooting of different cutting types of Abstract An experiment to evaluate different IBA levels and cutting types for achieving growth and rooting of Clerodendrum splendens was studied at Ornamental Horticulture Nursery, Department of Horticulture, The University of Agriculture Peshawar, Pakistan during the year 2012. In the experiment two types of cuttings were used i.e. stem cuttings and root cuttings and were treated with 0, 10, 20 and 30% IBA. Cuttings were then planted in polythene bags crammed with the mixture of clay, FYM and sand with the ratio of 1:1:1. IBA with the level of 20% showed best results regarding number of leaves per plant (14.0), number of roots per plant (9.8), root diameter (2.4mm) and survival percentage (60.0%). A significant increase in sprout length (29.1cm) and root length (24.8cm) were observed in cuttings treated with 10% IBA level over the control. Among cuttings, stem cuttings gave good results in all parameters as compared to the root cuttings. Interaction revealed that stem cuttings proved superior when treated with 20% IBA followed by 10% IBA, while root cuttings did not need IBA for rooting and resultant growth as these cuttings rooted and grew well without IBA. Hence it is recommended that stem cuttings need IBA at the rate of 20%, while root cuttings should be used without treating with IBA.
Bhartiya Krishi Anusandhan Patrika, Volume 38 Issue 4: 420-422 (December), 2023
Background: Queen's flower (Lagerstroemia speciosa) is an important ornamental plant that is utilised in residential landscape and avenue plantation for both public and private purposes around the world. Seed dormancy problem in Lagerstroemia which negatively affects germination. To overcome the challenges associated with seed propagation, researchers have explored rooted Lagerstroemia speciosa cuttings as an alternative method. Methods: Three concentrations of IBA (Indole-3-butyric acid) @ 1000 ppm, 2000 ppm and 3000 ppm were used to root initiation of Lagerstroemia speciosa stem cuttings. There were four treatments: 1000 ppm, 2000 ppm, 3000 ppm and control. Result: The 2000 ppm IBA treatment performed the better response in comparision to other treatments and recorded, 79 per cent rooting, followed by the treatment 3000 ppm IBA, (76 per cent rooting) and in the control, (68 per cent). In 2000 ppm IBA, the percentage of rooting, the number of roots per cutting and the length of the longest roots all are at their peak as well as showed the superior growth performance. The growth parameters of IBA-@ 2000 ppm treated cuttings revealed that this is the optimum IBA concentration for Lagerstroemia speciosa propagation trough cuttings in a semi-arid climate.
Journal of Forestry Research, 2011
In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala (Roxb) Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid (IBA) treatments. Significantly better rooting response (p ≤ 0.05) was observed with 0.1% IBA compared to control (0% IBA). The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference (p≤0.05). After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest (100 ± 0.00%) survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly (p≤0.05) among the treatments. Effects of three fertilizer treatments, viz. T0 (no fertilizer), T1 (10g Urea, 20g TSP, 10g MOP dissolved in 1 L water) and T2 (10g Urea, 20g TSP, 10g MOP dissolved in 2 L water) on initial growth of The online version is available at Responsible editor: Chai Ruihai stecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher (p≤0.05) under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.
IBA Induced Rooting Characteristics in Marathi Moggu Stem Cuttings: Evaluation Using SVI Concept
International Journal of Current Microbiology and Applied Sciences
Rooting ability in IBA induced stem cuttings in vegetative regeneration experiments largely depends on various parameters, which has been established using SVI (sprouting value index), a mathematical approach. Marathi-moggu the Bombax ceiba tree is a medicinal and avenue tree species of tropical India that has known to have poor regeneration through seeds and hence in the present study stem cuttings of saplings were used to generate clonal plant propagules. Field studies were carried out using three rooting medium at a location in Central Kerala, Peninsular India, for one year at four months regular interval. Three IBA (Indole 3butyric acid) concentrations 300 ppm, 500 ppm and 1000 ppm IBA, have been used and the data obtained was evaluated with SVI (sprouting value index) method in order to evaluate the suitable medium, which gives maximum results. The control cuttings do not recorded rooting. The results obtained indicate high SVI (sprouting value index), when stem root cuttings planted in root trainers with coir pith compost (RTCP) for all the three concentration of IBA applied. Sprouting percentage was increased and the delay in completion of sprouting/rooting initiation decreased by the use of IBA treatment.
African Journal of Plant Science, 2019
Duranta erecta is popularly grown as an ornamental plant in tropical and semi-tropical gardens. Current demands for the shrub have prompted the need for effective propagation methods. In this study, the influence of indole-3butyric acid (IBA) hormone concentration and media on rooting of D. erecta propagated from tip cuttings under greenhouse conditions were investigated. IBA hormone was used in three concentrations (2500, 5000, and 7500 ppm) and 0 ppm was the control. The study consisted of three media types (river sand, pine bark, a mixture of peat and perlite at a 1:1 ratio). The experiment was arranged in a 3×4 factorial layout in Randomized Complete Block Design replicated 3 times. Cutting survival, root length and number, shoot length and number were analysed. There was no interaction (P>0.001) between IBA concentration and media for all the measured parameters. The results showed that the optimum concentration of IBA is 5000 ppm, beyond which were inhibitory in all parameters except on root length. Type of media influenced survival and shoot number with pine bark giving the highest but did not affect root number, root length and shoot number.
Ocotea usambarensis Engl. is a high value timber tree that is threatened in Kenya due to overexploitation and poor natural regeneration. It seeds once in eight to ten years but most of its fruits are lost pre-maturely due to pest attack. In addition its seeds are recalcitrant. Alternative methods of propagation should be developed to complement the few seed available. A rooting trial was set up to determine the ability of Indole-3-butyric acid (IBA) to promote rooting in O. usambarensis coppice stem cuttings. The experiment was laid out in a complete randomised design and cuttings treated with IBA at 0, 60, 90 and 120mg/l concentrations. Each IBA concentration was replicated 3 times and each replicate had 10 cuttings. The experiment was incubated in a non-mist propagator with vermiculite as the rooting medium. Assessment for rooting was done on 84 th , 104 th and 136 th day. Data on rooting, number of roots and the length of the longest root and sprouting was collected and analysis done using Genstat. The highest rooting was 43.5% at 60 mg/l IBA and the least 6.7% in both 90 and 120 mg/l concentrations. However, the difference was not significant (p=0.264). The number of roots and length of the longest root were not significantly influenced by IBA treatment. However, the proportion of cutting that sprouted, the number and growth of sprouts were significantly decreased with increase in IBA concentration. The lack of significant positive increase in the rooting with IBA treatment suggests that O. usambarensis may have a relatively high level of endogenous auxins.
This study was carried out in the Agricultural College/ Salahalden University during the period between to investigate the effect of planting media (sand, sand: loamy soil, and mixture of sand: loamy soil: peat moss) 1:1:1 by volume, and IBA concentrations (0, 1000, 2000, 3000) mg.l-1 on rooting ability of stem cuttings of Ligustrum ovalifolium. The results show that the mixture of sand, loamy soil, peat moss is significantly superior than other media and give the highest values of roots number 7.45 root/plant, roots dry weight 0.33 gm/plant, shoot number 3.92 shoot/plant, leaf number 11.00 leaf/plant, and vegetative dry weight 3.76 gm/plant while the best rooting percentage 64.63% is obtained in cutting which are planted in peat moss and the longest root is for cutting which are planted in sand media. The 2000 and 3000 mg.l-1 IBA causes significantly increase in rooting percentage 68.92, 68.42%, longest root 3.41, 3.36 cm, roots dry weight 0.31, 0.31 gm, leaf number 9.67, 9.56 leaf/plant when comported with the other concentration. In the other hand the 3000 mg.l-1 is significantly superior in roots number 5.91 root/plant, shoots number 3.92 shoot/plant, and vegetative dry weight 2.82 gm/plant. The interaction between planting media and IBA concentrations case significantly variance in all rooting and shooting parameters and the highest values are for cutting treated with 2000, 3000 mg.l-1 and planted in deferent media.
Scientific Journal of Flowers and Ornamental Plants, 2020
The current study was conducted to evaluate the effects of indole-3-butyric acid (IBA) at 100 ppm with or without biostimulants (Agrobacterium rhizogenes, seaweed extract and coconut water) as either a spray or drench for 3 minutes or 1 hour on rooting and growth parameters of three cutting types (tip, middle and basal) of Conocarpus erectus L. The data showed that the rooting percentage, root and vegetative characteristics of rooted cuttings were significantly increased by all treatments of IBA and/or biostimulants compared to the untreated cuttings. The individual treatments of seaweed extract and coconut water were more effective than IBA or A. rhizogenes alone. Seaweed extract as drench with or without IBA surpassed those of seaweed extract treatments as spray. Besides, the response of cuttings treated with coconut water for 1 hour was better than coconut water for 3 min. Tip cuttings were significantly better than the middle and basal ones, especially in relation to rootability of cuttings. Maximum rooting percentage and shoot characteristics of plants were obtained from tip cuttings treated with IBA + coconut water for 1 hour, followed by IBA + seaweed extract as drench. The highest rooting percentage was associated with high endogenous levels of phenols, indole acetic acid (IAA) and gibberellic acid (GA) as well as the lowest abscisic acid (ABA) level in cutting tissues and the anatomical structure of rooted cuttings. So, it can be recommended treating tip cuttings of C. erectus with either coconut water for 1 hour or seaweed extract as drench combined with IBA for enhancing the rooting percentage, root and shoot growth parameters as well as content of endogenous root promoting substances.
Bangladesh Research Publications Journal, 2013
An experiment was carried out at the Department of Horticulture at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the investigation of the performance of different concentration of Indole-3-Butyric Acid (IBA) for sprouting and rooting potential of Bougainvillea spectabilis stem cutting during the period from May 2013 to August 2013. Five treatments viz. T0: Control; T1: IBA in dust form; T2: IBA 500-ppm; T3: IBA 1000-ppm and T4: IBA 2000-ppm were exploited on the experiment with five replication. In case of T2, T3 and T4 cuttings were soaked for 24 hours on the respective solutions. From the experiment it was found that IBA play an important role for successful asexual propagation of bougainvillea through cutting. Better performance was found when cut stems were soaked for 24 hours before the establishment of stem cutting. But performance was inhibited by increasing concentrations of IBA in terms of all of the features on which data had taken. During the experimental period, data was taken on different attributes and T3 (1000-ppm) was performed as the best among the treatments which as followings: days to first rooting (4.0), days to first sprout bud initiation (5.3), number of sprout bud/cutting (3.6), number of leaves/cutting (35.2), length of sprout (15.0 cm), number of branches/cutting (4.7), number of root/cutting (64.2), number of sub root/cutting (25.8), longest root length (33.2 cm), longest root diameter (0.51 mm), rooting % (100%), survival % of rooted cuttings (100%). Though 100% rooting was also found in 500-ppm and 2000-pmm but only 1000-ppm was resulted 100% survival of rooted cuttings.
Effect of IBA and Biopreparations on Rooting Performance of Cotinus coggygria Scop
Rooting is an important step in vegetative plant propagation; failure to form roots always creates losses. EU-imposed restrictions on chemicals used in plant production create need for test of new natural products that may enhance rooting in commercial production. This study tested the effect of two biological preparations: AlgaminoPlant and Route on the difficult-to-root smokebush in conventional propagation and in micropropagation. Stem cuttings were sprayed with the biostimulators in different combinations and their efficiency was compared to the commonly used synthetic auxin β-indolyle butyric acid (IBA) used either as a commercial rooting powder Rhizopon AA or a foliar application of the aqueous solution of IBA. The success rate was measured by the rooting percentage and the degree of root ball development. Treatment with a aqueaous solution of IBA was more effective than Rhizopon AA. AlgaminoPlant and Route enhanced rhizogenesis so they are viable substitutes for synthetic auxins in conventional propagation of C. coggygria. In in vitro propagation, microshoots were cultured on Murashige and Skoog (MS) medium supplemented with IBA or biostimulators. The highest rooting rate was recorded for the 10-30 mg dm-3 IBA. AlgaminoPlant added to the medium increased the number of roots produced by microcuttings while Route had a deleterious effect. The experiments show that bio-stimulators are commercially viable substitutes for commonly used hormones in nursery handling of even difficult stocks.