Influence of Culture Media, Temperature and pH on Colletrotrichum gleosporioides, Isolated from.pdf (original) (raw)
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The paper gives an account of the variations in nutritional and physiological characteristics found in different isolates of Colletotrichum gloeosporioides causing anthracnose disease of papaya, in India. The pathogen under study varied in its ability to utilize different carbon and nitrogen sources. Fructose was found to be the best source of carbon for the growth and sporulation of most of the isolates. Among the nitrogen sources tested, aspartic acid supported the maximum growth of isolates followed by potassium nitrate and proline. In contrast to this, isolates sporulated better in media containing potassium nitrate, ammonium nitrate or sodium nitrate as the sole nitrogen source. The response of different isolates to different temperature levels were found to be vary. However, most of the isolates preferred temperature range of 28 0 C to 30 0 C for the growth and sporulation when grown on Richard's agar medium. C. gloeosporioides isolates grew well at pH 5 while sporulation was better at pH 6.
The present study was carried out to investigate the influence of physiological factors on vegetative growth and sporulation of Colletotrichum gloeosporioides causing anthracnose rot of mango. Among ten culture media, the highest colony growth of the fungus was observed on corn meal agar, malt extract agar, Sabouraud's agar and oat meal agar followed by potato carrot agar. Significantly higher number of conidia was observed on Waksman's agar and corn meal agar, followed by oat meal agar. Corn meal broth medium produced maximum dry mycelial weight of the test pathogen followed by Waksman's and Czapek's broth. The incubation of the test fungus under '12 h light 12 h dark' produced significantly more colony growth than other treatments. The minimum, optimum and maximum temperatures for C. gloeosporioides were 20 °C, 30 °C and 40 °C, respectively. It grows best at pH 5 followed by pH 6.
Journal of Pharmacognosy and Phytochemistry, 2019
Anthurium is tropical ornamental plant and it is one of the most valued cut flowers which have a great export potential. Anthracnose or spadix rot disease leads to massive flower loss to anthurium growing farmers. Survey was conducted for anthurium anthracnose disease during 2017-2018 at anthurium growing areas of Tamil Nadu. Maximum disease incidence was observed in Thandikudi (56.66 PDI) and Pandrimalai (54.43 PDI) village of Dindugal district. The fungus C. gloeosporioides was isolated and the morphological variations were studied among the isolates. The result revealed that all the isolates of C. gloeosporioides were circular and wavy pattern and the topography were observed as raised fluffy growth. The I piiboyfgCg1 of Thandikudi isolates recorded 89.12 mm radial mycelial growth with excellent sporulation and least mycelial growth on ICg6 (64.15mm) with poor sporulation. The length of the conidia ranged from10.28-16.28 μm. Highest length of conidia was observed in ICg1 isolates (16.28 μm) followed by ICg10 (15.39 μm). Width of the conidia ranged from 5.77-3.31 μm. Isolate ICg1 recorded the highest width of conidium (5.77 μm) and was followed by ICg10 (5.23 μm).
Molecular Biotechnology, 2008
Colletotrichum capsici is an important fungal species that causes anthracnose in many genera of plants causing severe economic losses worldwide. A primer set was designed based on the sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a conventional PCR assay. The primer set (CcapF/ CcapR) amplified a single product of 394 bp with DNA extracted from 20 Mexican isolates of C. capsici. The specificity of primers was confirmed by the absence of amplified product with DNA of four other Colletotrichum species and eleven different fungal genera. This primer set is capable of amplifying only C. capsici from different contaminated tissues or fungal structures, thereby facilitating rapid diagnoses as there is no need to isolate and cultivate the fungus in order to identify it. The sensitivity of detection with this PCR method was 10 pg of genomic DNA from the pathogen. This is the first report of a C. capsici-specific primer set. It allows rapid pathogen detection and provides growers with a powerful tool for a rational selection of fungicides to control anthracnose in different crops and in the post-harvest stage.
Global Journal Of Botanical Science
In the present study, different isolates of Colletotrichum gloeosporioiedes were collected from different mango growing areas of Bangladesh. Pathogenecity was proved by using healthy excised leaves in laboratory conditions. Effects of mycelial growth and sporulation (formation of acervuli) on six different culture media were tested in vitro. The results revealed significant differences (p<0.05) mycelia growth among the media. The results also indicated that the mycelial growth was maximum in Potato Dextrose Agar (12.8mm) media and the lowest in Green Bean Agar (8.0mm). The study disclosed significant difference (p<0.05) of acervuli formation among the media. Among the 6 different media the maximum acervuli were produced in HLEA (4.0 per square. cm) medium. But the sporulating characters of the fungus were not produced well in PDA and HLEA media rather than OMA. Thus, to find out the best composition of OMA for further research was conducted to obtain better growth and acervuli production and sporulating characters. The results revealed significant difference (p<0.05) of different characters among the concentration of OMA, and 20g/L OMA produced significantly maximum setae, conidia and sticky masses of conidia than others. The study suggested that Oat Meal Agar (20g/L) composition was the best medium for the sporulation of C. gloeosporioiedes (Setae, conidia and sticky masses of spores).
SABRAO Journal of Breeding and Genetics
Anthracnose forms a group of fungal diseases that affect a variety of crop plants in warm and humid areas. Anthracnose causes the wilting, withering, and dying of tissues of plants, commonly infecting the developing shoots, leaves, and fruits. It critically affects the quality and production of Carica papaya fruits in Bali, Indonesia. The pathogenic fungi belonging to the genus Colletotrichum cause anthracnose fungal disease. Research on morphology and molecular identification of pathogenic fungi is very important to determine the type of pathogenic fungus that causes anthracnose disease in the papaya fruit. The latest study aims to identify the pathogen that caused anthracnose on papaya fruits in Bali, Indonesia, carried out during July – December 2021 at the Belok Village, District of Petang, Badung Regency, and in Laboratory of Biochemistry, Udayana University, Badung Regency, Bali, Indonesia. The study used Koch's postulates to confirm the pathogenicity and continued with ma...
OnLine Journal of Biological Sciences, 2012
Colletotrichum gloeosporioides causing anthracnose which is a serious post harvest disease in mango accounting for 15-20% loss. The variation in nutritional and physiological characteristics among five isolates of C. gloeosporioides collected from different agro-climatic regions of India was investigated. All the isolates showed differential response in requirements of media, temperature and media pH for growth and sporulation. Malt Extract Agar (MEA) medium was best suited for growth in terms of radial mycelial diameter for all the isolates. Among the studied isolates, Cg 72 (from Maharashtra) showed more virulence and maximum sporulation (137.5×10 3 mL −1) at 28°C and media pH 6. Maximum growth and virulence at 28°C was observed with Cg 62 isolate. Media of pH 6 was found to be most suitable for the growth of respective isolates (s), but Cg 62 which was collected from Bihar found most virulent in this experiment.
Journal of Pharmacognosy and Phytochemistry, 2017
Survival of Colletotrichum gloeosporioides (Penz.) Penz., & Sacc. the causal organism of anthracnose disease of mango was studied at the CCS Haryana Agricultural University, Hisar. The naturally infected mango leaves collected from mango orchard were wrapped in synthetic nets and buried soil at different depths and durations under screen house conditions. The pathogen survived for seven month in soil as conidia or mycelium in infected debris under adverse conditions. As the depth and duration increased, there has been a decrease in viability of the fungus indicating its long survival on the soil surface only. The fungus could not survive at 5 cm depth after seven months of burial in comparison to 81.6 per cent survival at same depth after one month of burial. At 5 cm and 10 cm depth conidia or mycelium remained viable for five months at 2% urea concentration, however, fungus remained viable up to four months at 5% urea concentrations.
First Report of Colletotrichum chrysophilum Causing Papaya Anthracnose in Mexico
Plant Disease
Anthracnose, caused by Colletotrichum spp., is the most important fungal disease of papaya (Carica papaya L.) worldwide. In March 2020, mature papaya fruit (cv. Maradol) showing typical symptoms of anthracnose were observed in an orchard located in Pinotepa Nacional, Oaxaca, Mexico. Disease incidence of 100 papaya plants surveyed in the orchard was estimated at about 45%. Initially, small and water-soaked lesions appeared on the fruit surface, which later enlarged to circular sunken lesions with translucent light brown margins. On advanced infections, salmon-pink masses of spores were observed on the lesions. Twenty Colletotrichum-like colonies were consistently isolated on potato dextrose agar (PDA) medium at 25°C in the dark for 6 days and 10 monoconidial isolates were obtained. An isolate was selected as representative for further characterization. The isolate was deposited as CPM-H4 in the Culture Collection of Phytopathogenic Fungi of Plant Pathology Laboratory of the CIIDIR-Oa...