Uterine Leiomyosarcoma Has Deregulated Cell Proliferation, but Not Increased Microvessel Density Compared with Uterine Leiomyoma (original) (raw)

1997, Gynecologic Oncology

leiomyoma) and malignant uterine smooth muscle tumors Objective. To investigate the differences of biological aggressive-(leiomyosarcoma). Uterine myoma is usually benign lesions ness in terms of proliferating cell nuclear antigen (PCNA) expreswith less than 5 mitoses per 10 HPFs. In contrast, if ú10 sion, cell proliferation, and microvessel density between uterine mitoses per 10 HPFs are found, the tumor is considered leiomyosarcoma and leiomyoma. malignant [1]. Uterine sarcomas, as a group, are character-Study design. All patients with uterine leiomyosarcoma undergoized by rapid clinical progression, and they carry a poor ing surgery at National Cheng Kung University Hospital were overall prognosis. It is generally believed that the number eligible. Forty-four patients with uterine myoma were also studied of mitoses per 10 HPFs seems to be the most reliable preas the benign counterpart. The paraffin-embedded slides were dictor of biological behavior. However, the mitotic count is stained with hematoxylin and eosin to confirm the presence of still considered to be a fairly crude index of proliferation, tumor and to quantitate mitoses, PC 10 for measurement of PCNA and suffers from limitations [2]. expression, MIB 1 for measurement of cell proliferation, and factor VIII for quantitation of microvessel density. The immunohisto-Proliferating cell nuclear antigen (PCNA), a 36-kDa nuchemical findings of the slides were correlated with clinocopathoclear protein, is a necessary component of the machinery logic findings of the patients, and the data were analyzed by either that copies DNA so that cell division can take place [3, 4].