Sperm Microinjection Techniques Do Not Increase the Incidence of Abnormalities in Offspring (original) (raw)
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Population, 2003
In response to involuntary infertility, which affects 15% to 20% of couples, the techniques of in vitro fertilization (IVF) have been developed. Between 1982 and 2000, 85,000 children were born in France thanks to IVF (estimates based on combined analysis of the FIVNAT survey and administrative data). Five world reports and two European reports have been produced on IVF, but their use remains limited by incomplete data for regions such as southern and eastern Europe. The success rates with IVF are around 15% to 20% of pregnancies obtained per retrieval. However, these rates decline rapidly as the woman’s age rises and when the man’s sperm has severe abnormalities. In cases of severe male factor infertility, artificial insemination by donor (AID) was for long the only medical solution, but it raises the problem of accepting sperm from a donor. Since 1992, a new IVF technique, intracytoplasmic sperm injection (ICSI) is available. This technique has been widely adopted in France and in many other countries: in 1998 it represented 30% to 60% of assisted fertilizations. Despite this large development, numerous questions remain concerning the consequences of the technique, particularly regarding the short- and long-term health of children conceived by ICSI.
Fertility and sterility, 2017
To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). Observational longitudinal cohort study. Private in vitro fertilization (IVF) center. A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA). ICSI with ejaculated/surgically retrieved sperm, blastocyst culture, trophectoderm-based quantitative polymerase chain reaction PGT-A, and frozen-warmed euploid embryo transfer (ET). The primary outcome measures were fertilization, blastocyst development, and euploidy rates; the secondary outcome measures were live birth and miscarriage rates. Perinatal and obstetrical outcomes were monitored as well. A total of 9,042 metaphase II oocytes were inseminated. The fertili...
A retrospective follow-up study on intracytoplasmic sperm injection
1999
Purpose: Genetic aspects of male subfertility and the novelty of intracytoplasmic sperm injection (ICSI) as a new technique can influence the development of zygates and children bom after ICSI. Therefore, we evaluated the outcome of ICSI compared to in vitro fertilization (IVF). Methods: Data from medical records of 233 total pregnancies and the follow-up of 132 children born after IVF and 120 after ICSI were retrospectively analyzed. Results: No differences were found between ICSI and IVF for early embryonic development and obstetric outcome. In both groups the rate of women undergoing prenatal chromosomal diagnosis was low, 30.0%. The congenital malformation rate was 3.0% after IVF and 1.7% after ICSI, which was not significantly different. Follow-up on development of children born after IVF and ICSI also showed no significant differences.
Intracytoplasmic sperm injection (ICSI) in 2006: Evidence and Evolution
Human Reproduction Update, 2007
The introduction of intracytoplasmic sperm injection (ICSI) in 1992 has dramatically changed the management of severe male infertility. In severe male infertility, live birth rates with ICSI are superior to those with other nondonor treatments. In non-male infertility, however, pregnancy rates are not better with ICSI than with in vitro fertilization (IVF). With obstructive or non-obstructive azoospermia, reasonable pregnancy rates are now possible with ICSI after recovery of sperm from the testes followed by ICSI. Genetic counselling is indicated for severe male infertility, whether or not ICSI is considered. ICSI is indicated in preimplantation genetic diagnosis (PGD) to avoid contamination by extraneous DNA in the case of PCR-based testing and to increase the number of embryos available for testing. In turn, PGD may be indicated in pregnancies that are at high risk of aneuploidy because of genetic factors associated with azoospermia. As with IVF, not all couples succeed, but 2% of couples with failed ICSI cycles will conceive without treatment. ICSI outcome studies indicate that there is a significant increase in prematurity, low birthweight, and perinatal mortality associated with single and multiple births, similar to the outcomes of conventional IVF. However, as evidenced in long-term follow-up studies, the higher rates of urogenital abnormalities and increased use of healthcare may be associated with paternal characteristics.
The Impact of Intracytoplasmic Sperm Injection in Non-Male Factor Infertility—A Critical Review
Journal of Clinical Medicine, 2021
Intracytoplasmic sperm injection (ICSI) was originally designed to overcome barriers due to male factor infertility. However, a surveillance study found that ICSI use in non-male factor infertility increased from 15.4% to 66.9% between 1996 and 2012. Numerous studies have investigated fertilization rate, total fertilization failure, and live birth rate per cycle (LBR), comparing the use of ICSI versus conventional in vitro fertilization (IVF) for non-male factor infertility. The overwhelming conclusion shows no increase in fertilization rate or LBR per cycle with the use of ICSI for non-male factor infertility. The overuse of ICSI is likely related to the desire to avoid a higher rate of total fertilization failure in IVF. However, data supporting the benefit of using ICSI for non-male factor infertility is lacking, and 33 couples would need to be treated with ICSI unnecessarily to avoid one case of total fertilization failure. Such practice increases the cost to the patient, increa...
Fertility and Sterility, 2003
Objective: To verify whether microinjection into retrieved oocytes of motile spermatozoa with morphologically normal nuclei, strictly defined by high power light microscopy (ϫ Ͼ6000), improves the IVF/ intracytoplasmic sperm injection (ICSI) pregnancy rate in couples with repeated ICSI failures. Design: Comparative prospective study testing routine IVF/ICSI outcome parameters against those of modified ICSI based on morphological selection of spermatozoa with normal nuclei. Setting: Male factor fertility laboratory and IVF center. Patient(s): Sixty-two couples, with at least two previous consequent pregnancy failed ICSI cycles, underwent a single ICSI trial preceded by morphological selection of spermatozoa with normal nuclei. Fifty of these couples were matched with couples who underwent a routine ICSI procedure at the same IVF center and exhibited the same number of previous ICSI failures. Intervention(s): Standard ICSI and modified ICSI. Main Outcome Measure(s): ICSI pregnancy rate.