Characterization of Diarrhoeagenic Escherichia coli isolates in Jordanian Children (original) (raw)
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Science Journal of University of Zakho, 2020
Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types of diarrhea in children especially in developing courtiers. Objectives: This study was primarily aimed to isolate and bacteriological characterizing of E coli from diarrheic infant stool and to investigate their antibiotic resistance patterns and then using molecular identification of DEC pathotypes for better discrimination. Methods: Total of 400 fresh stools specimens were collected from children with diarrhea in Heevi Hospital in Duhok city, Iraq. The samples were cultured on selective media such as (MacConkey and MacConkey sorbitol agar). Colonies were identified through biochemical reaction and VITEK 2 system and then antibiotic susceptibility profiles were determined. Results: A total of 349(87.2%) samples were yielded positive for growth of E coli. Out of these, 50 phenotypically-identified E coli were then subjected to PCR assay targeting certain virulence factors (alt, eae, sxt1 ...
Jundishapur Journal of Microbiology, 2015
Background: Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea. Objectives: This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children with diarrhea between 2007-2008 in Tehran, Iran. Materials and Methods: EAEC strains were examined for virulence plasmid genes (aap, aggR, and aatA), biofilm formation, and drug resistance. In addition, pulsed-field gel electrophoresis (PFGE) profiles of these strains were determined. Results: Significant percentage of local EAEC strains carried the virulence plasmid genes and formed biofilms. In addition, these strains showed high resistance to ampicillin (100%), tetracycline (65.7%), streptomycin (58.7%), chloramphenicol (52.6%), and trimethoprim/ sulfamethoxazole (51.7%) and had different PFGE patterns. Conclusions: These results indicated that EAEC strains isolated from Iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries.
Journal of Medical Microbiology, 2014
Diarrhoea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Diarrhoeagenic Escherichia coli (DEC) is an emerging agent among pathogens that cause diarrhoea. Between March 2011 and January 2012, a total of 600 stool specimens from children younger than 5 years of age (450 with and 150 without diarrhoea) were investigated for enteroaggregative E. coli (EAEC), enterohaemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC) using PCR. Antimicrobial susceptibility testing was performed following Clinical and Laboratory Standards Institute guidelines. The prevalence of DEC pathotypes was 30.4 % (137 patients) and 12 % (18 patients) in the diarrhoea group and the control group, respectively. The most frequently isolated pathotype in diarrhoeal children was ETEC. This pathotype was detected significantly more often in children with diarrhoea (14.4 %) than in children without diar...
Journal of Pure and Applied Microbiology, 2018
Diarrheagenic Escherichia coli still an important pathogen that cause diarrhea which lead to hospital admissions and death specially in children. In order to identify the common pathotypes of E. coli via investigate different virulence genes. A total of 210 stool samples were collected from children under five years presented with diarrhea from different hospitals and private clinics in Wasit province, Iraq, on the other hand, 40 stool samples were collected from healthy children considered as control group. regarding to culture, biochemical tests and API 20E results 100 isolates were supposed to be E. coli. The DNA were extracted to that 100 isolates from diarrheal cases and for 40 isolates of control, concentration of DNA samples were between (50-360 mg/µl) and the purity between (1.8-2). All isolates studied for detectionvirulence gene of five Diarrheagenic Escherichia Coli strains based on using multiplex Polymerase Chain Reaction technique, by amplified 13 primer (eaeA, bfpB, aggR, astA, pic, hly, stx1, stx2, invE, ipaH, elt, estIa, estIb), and showed the distribution of the strains and its susceptibility to antibiotics. The most frequent pathotypes was Enteropathogenic E.coli 19/42 (45.3%)) with 9 typical and 10 atypical, followed by Enteroaggregative E. coli 17/42 (40.5%), Enterotoxigenic E. coli 3/42 (7.1%), Enteroinvasive E. coli 3/42 (7.1%), and 0/42 (0%) in Shigatoxin producing E .coli and no DEC in all control patients. The highest resistance to antibiotics was (95.2%) to Amoxicillin and Ampicillin, respectively, Sulfa-Trimethoprim 92.9%, followed by 85.7% for Tetracycline and Cephalothin, Ceftriaxone 81% and Cefotaxim "clavulanic acid 71.4%. While the lowest resistance was to Chloramphenicol (19 %), Ciprofloxacin (16.7%), Amikacin (7.1%) and no resistance was detected toward Imipenem. We can conclude in this study, multiplex PCR is a swift, and accurate procedure can be used for Diarrheagenic E.coli identification and isolation successfully of strains.
J Infect Dev Ctries 2014; 8(5): 589-596. doi:10.3855/jidc.4077.
Introduction: Little information is available regarding the significance of enteroaggregative Escherichia coli (EAEC) in pediatric diarrhea in Egypt. Methodology: Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction (PCR). Diarrheagenic E. coli were grouped phylogenetically using PCR and tested for their susceptibility to antibiotics using the disk diffusion method. Isolates designated as EAEC were examined for eight virulence factors (VFs) using PCR. Results: EAEC was detected in 19 (30.7%) and 4 (9.3%), EPEC in 2 (3.2%) and 1 (2.3%), and ETEC in 2 (3.2%) and 0 (0.0%) diarrheic and control children, respectively; STEC and EIEC were not detected. Only EAEC was significantly isolated from diarrheic children compared with controls (p < 0.01, OR = 4.31).Three or more VFs (multivirulent isolates) were found in 52.6% and 50% of EAEC isolated from diarrheic children and controls, respectively. More than 73% (17/23) of EAEC isolates were identified as belonging to phylogenetic group D. Multiple-antibiotic resistance (resistance to three or more drugs) was observed in more than 91% of EAEC. Conclusions: Multivirulent EAEC is a significant causative agent of pediatric diarrhea in Egypt, with the majority of isolated EAEC belong to phylogenetic group D. Multiple-antibiotic resistance among EAEC has the potential to be a serious public health problem for the country.
Journal of Medical Microbiology, 2006
The present study was performed to investigate the contribution of typical and atypical enteropathogenic Escherichia coli (EPEC) as a cause of infectious diarrhoea among children less than 10 years old in Iran. During the summer months, 247 specimens from children with diarrhoea and 1108 from asymptomatic children were analysed for the presence of EPEC and other bacterial pathogens. Potential enteric pathogens were identified in 140 cases of children with diarrhoea (56.7 %). EPEC was the most frequently identified agent (111 cases), followed by Shiga toxin-producing E. coli (13), Shigella (9), Salmonella (6) and Aeromonas sp. (1). EPEC isolates were examined for the presence of eaeA, bfpA and stx genes by PCR. EPEC isolates were classified as typical (eaeA + bfpA +) or atypical (eaeA + bfpA −). Typical EPEC was diagnosed in 35 cases (11.8 %), compared with 8 (0.4 %) in the asymptomatic group (P<0.05). Atypical EPEC strains were isolated from 23 cases (9.3 %), compared with 13 (1....
Egyptian Academic Journal of Biological Sciences E. Medical Entomology & Parasitology, 2022
Escherichia coli (E. coli) is one of the most frequent opportunistic pathogens. We aimed to carry out this study on antimicrobial resistance pattern and phylogenetic background of E. coli isolated from children who have diarrhea in different hospitals of the Iraqi Kurdistan region. A total of 200 fecal samples of children (52.5% male and 47.5% female) were included. Stool samples were cultured on selective media to isolate E. coli bacteria. The antibiotic susceptibility testing and molecular characterization of the isolates were carried out. Antimicrobial susceptibility testing was performed by disc diffusion test disc PCR was used to detect five antimicrobial resistance genes coding for β-lactamases (blaTEM, and blaCTX, blaOXA, blaSHV, blaCMY) to study phylogenetic grouping of E. coli. The Escherichia coli were highly resistant to β-lactam antibiotics, and the highest resistance was seen to Cefotaxime (45.4%). According to the phylogenetic grouping of E. coli isolates, group B2 was the dominant group and blaOXAgene (57.5%) was the predominant resistance genes among E. coli isolates. This study demonstrated the clinical concern of E. coli in children. The E. coli are highly resistance to common beta-lactam antibiotics and there are high rates of the resistance genes among E. coli isolates in children. An antibiotic surveillance would be useful continuously control and prevention of infectious diarrhea in children.
Bosnian journal of basic medical sciences / Udruženje basičnih mediciniskih znanosti = Association of Basic Medical Sciences, 2009
Diarrhoeal disease is a major cause of illness and death among infants and young children worldwide. Among the Escherichia coli (E. coli) causing intestinal diseases, there are six well-described categories: enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC) and enterotoxigenic E. coli (ETEC). The aim of the present study was to investigate the relative contribution of different groups of diarrhoeagenic E. coli (DEC) in paediatric patients with diarrhoea. Clinical stool specimens from 380 children with diarrhoea, with ages ranging from birth to < 12 years, were selected for the study over a period of 17 months (August 2007 to December 2008). The study showed that 85/380 children (22%) had diarrhoea due to diarrhoeagenic E. coli. The most prevalent was enteropathogenic E. coli (EPEC) isolated from 46/85 paediatric patients (54%), followed by enterotoxigenic (ETEC) is...
Journal of Infection and Chemotherapy, 2013
The aim of this study was to investigate the genetic diversity and class 2 integron content of typical and atypical enteropathogenic Escherichia coli (EPEC) strains isolated from children less than 5 years of age. Biochemical tests and serogrouping were performed for identification of isolated strains, and each isolate was tested for susceptibility to 7 antimicrobial agents. The identity of EPEC and their class 2 integron content was confirmed by PCR analysis and sequencing. Subtyping of Escherichia coli spp. was performed through pulsed-field gel electrophoresis (PFGE) analysis. All EPEC strains were resistant to 6 antimicrobial agents except for gentamycin. The most prevalent serogroups among EPEC strains were found to be members of O86 and O127 serogroups (37.7 %) and O44, O125, and O128 (42.8 %). The majority of our EPEC isolates (60.7 %) were identified as atypical. Among the total 28 isolates, 4 (14.2 %) harbored a class 2 integron 1,500 or 2,300 bp in size, corresponding to dfrA1-sat1 and dfrA1-sat1-aadA1 resistance gene cassette arrays, respectively. PFGE analysis showed an extensive diversity among the isolates. No PFGE clustering was observed according to bundle-forming pilus (bfp) bacteria, suggesting that PFGE analysis could not discriminate between typical and atypical EPEC strains. The high ratio of antibiotic-resistant strains and the large heterogeneity among EPEC isolates with low prevalence of class 2 integrons signify the need to examine for other mechanism(s) involved in conferring resistance in typical and atypical populations of EPEC.