Sero-prevalence of Toxoplasma gondii in Pregnant Women and Slaughtered Pigs of Chitwan District. (original) (raw)
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Seroprevalence of Zoonotic Parasites in Pigs Slaughtered in the Kathmandu Valley of Nepal
Vector-Borne and Zoonotic Diseases, 2013
For several years, the demand for pork has been on the rise in Nepal. To assess the importance of pork as a carrier of zoonotic agents, we performed a cross-sectional study in the Kathmandu Valley of Nepal, in which we serologically determined the infection status of slaughtered pigs with regard to three of the most important parasites transmitted through pork consumption: Trichinella spp., Taenia solium cysticerci, and Toxoplasma gondii. From 2007 to 2010, 742 pigs were sampled at slaughter, of which 0.1% (95% confidence interval [CI] 0.0-0.7%) were found positive for Trichinella infection, 13.8% (95% credibility interval [CrI] 0.8-28.5%) for T. solium cysticercosis, and 11.7% (95% CI 5.2-17.5%) for Toxoplasma infection. Further monitoring of the related animal and human disease burden and strengthening of food safety protocols throughout the pork production chain are strongly recommended.
Taenia solium cysticercosis survey at a slaughterhouse in Kampala, Uganda
Revue d’élevage et de médecine vétérinaire des pays tropicaux
A survey was conducted at Wambizi slaughterhouse in Kampala City, Uganda, to ascertain the prevalence of Taenia species infection in pigs via serology, as well as the role of meat inspection in control of these parasitic conditions in carcasses of slaughtered pigs. A total of 620 pigs were sampled using simple random selection during three months. Individual pig data including sex, breed, district of origin, antemortem clinical signs and postmortem lesions were recorded. Blood samples were collected for serological assays. A questionnaire was used to capture perceptions of meat inspectors and pig traders on T. solium cysticercosis. Of the 620 pig inspected carcasses, only four were observed with T. solium cysts and none had T. hydatigena cysts. Up to 67 (10.8%) pigs tested positive by B158C11A10/B60H8A4 Ag-ELISA serology. Seroprevalences were significantly different between districts (χ2 = 45.98; p < 0.001) with pigs from the Eastern districts having the highest seroprevalence. T...
Parasite Epidemiology and Control
Background & methods: Taenia solium a zoonotic tapeworm, responsible for neurocysticercosis in humans is a major public health threat, being a leading cause of acquired epilepsy in endemic regions. Eastern and southern African nations have experienced a recent rapid growth in pig production, including small-scale, free-range systems, with an accompanying increased risk of T. solium transmission. Seven hundred blood samples were collected from randomly selected pigs presented for slaughter at one of the largest porcine abattoir supplying unprocessed pork to Nairobi city and its surroundings. The samples were tested using an antigen ELISA to determine the prevalence of infection with Taenia spp. Results: The prevalence, adjusted for diagnostic test characteristics, was estimated to be 4.4% (95% CI: 1.9-7.1) with no significant statistical difference by pig sex or age. Infection with Taenia spp. was detected in pigs from all regions of the country supplying pigs to this slaughterhouse. Official post-mortem inspection did not detect cysticercosis in the duration of the study. Therefore, all the carcasses entered the food chains of Nairobi (70%), or neighboring counties (30%). Conclusions: Circulating antigens of Taenia spp. were detected in pigs slaughtered in one of the largest porcine slaughterhouses in Kenya, which receives pigs from several regions in the country. This is an indication that pigs entering the value chain are raised under poor husbandry conditions and that pork consumers in Nairobi and its surroundings may be exposed to the important zoonotic parasite. Whilst further research utilizing full carcass dissection is required to confirm T. solium positive cases, interventions to improve food-safety throughout the pork value chains in Kenya should be seriously considered.
Background and Aim: Pig farming is a livelihood activity undertaken by many rural communities in Tanzania. However, pigs in rural communities become infected with Taenia solium, a zoonotic parasite leading to porcine cysticercosis (PCC). Thus, routine meat inspection is fundamental in ensuring that the meat consumed is PCC-free. However, routine meat inspection is constrained by low sensitivity as a diagnostic test. Regardless of its low sensitivity, at the local level, no substitute tool would potentially lead to meat being risk-free for human infection. This study aimed at quantifying the risk of humans getting exposed to T. solium taeniasis through the consumption of pork approved safe for human consumption by employing a quantitative risk assessment (QRA) model. Materials and Methods: A cross-sectional study employing a quantitative risk assessment technique was conducted to quantify the risk of human infection in Mpwapwa District through exposure to T. solium infection through consumption of officially inspected pork. The input parameters in this study were simulated in @risk software to obtain the risk of exposure and the risk factors for exposure to T. solium taeniasis. Results: The risk of one getting exposed to T. solium taeniasis/cysticercosis (TSTC) through consumption of pork approved for human consumption was found to be 0.018 (95% confidence interval [CI] = 0.00-0.0250). Likewise, the probability that a cyst is localized in a pork portion was found to be the most influencing input risk factor of getting exposed to TSTC. Furthermore, the probability of developing T. solium taeniasis was estimated to be 0.73605 (95% CI = 0-0.950) when infected undercooked pork portion is consumed and 0.99652 (95% CI = 0.98161-0.99908) from consuming raw pork portion. Likewise, about 47 (95% CI = 42-52) people who consumed undercooked pork and 26 (95% CI = 22-30) who consumed raw pork would get infected in Mpwapwa District per year. Conclusion: The results from this study are anticipated to create public awareness of the problem and promote the use of one-health approach in the control and prevention of the consumption of infected pork.
BMC Veterinary Research
Background: Toxoplasma gondii, an important food borne zoonotic parasite, infects almost all warm-blooded animals including pigs. People primarily become infected with T. gondii via consuming meat of infected animals. Status of T. gondii is largely unknown in pigs in India including northern regions. We, therefore, determined the prevalence of T. gondii infection in pigs from North India. Results: DNA of T. gondii was detected in 6.7% (54/810) of the tested slaughter pigs. Highest prevalence was observed in pigs from Punjab (8.2%) followed by Chandigarh (5.3%) and Uttarakhand (4.8%). Phylogenetic analysis revealed that the isolates from pigs had 96-100% nucleotide identity with Type I RH strain (AF179871), 96-99.7% with VEG type III strain (LN714499) and 67-72% with type II ME 49 strain (XM002370240). However, low level of polymorphism in the targeted B1 gene did not allow the determination of the clonal lineages of the isolates. Antibodies against T. gondii was reported in 48.3% (73/151) of the sera obtained from pigs slaughtered at Chandigarh abattoir, and scavenging by pigs was a significant risk factor.
PLOS Neglected Tropical Diseases
Background Taenia solium (T. solium) cysticercosis remains a neglected zoonotic disease in India. The current study was planned to estimate the prevalence of T. solium porcine cysticercosis in the Punjab state of India, to compare this prevalence with the disease prevalence in pigs reared outside Punjab and to assess the distribution of the parasite in pig carcasses. Methods Two slaughter shops were selected in each of the 22 districts of Punjab. Pigs slaughtered on the day/s of inspection were post-mortem inspected to identify the presence of T. solium cysts. Estimated true prevalence was estimated by taking into account the diagnostic sensitivity (38%) and specificity (100%) of post-mortem inspection using the Rogan-Gladen estimator. Positive carcasses were purchased and brought to the laboratory to assess the tissue distribution of T. solium cysts and to conduct PCR targeting large subunit rRNA gene, internal transcribed spacer 1 gene, ITS1 gene and Cytochrome oxidase I gene. The selected PCR products were submitted for sequencing and phylogenetic analyses were performed. Findings We contacted 71 shop owners to achieve a sample of 44 shops for the study.
Journal of Life Sciences
Taenia solium is an important food-borne pathogen worldwide and is emerging as a serious public health risk in both rural and urban communities where pigs are raised and consumed. Adult tapeworms are found in the intestines of humans while the developmental larval forms occur in the muscles and organs constituting cysticercosis of pigs and humans. Cysticercosis has a worldwide distribution, mainly related to poor hygiene and sanitation and consumption of infected pork. Pigs get infected through consumption of food and contaminated with human faeces containg eggs. In recent years pork consumption has increased with the opening up of pork eating centres. Porcine cysticercosis has previously been reported in Kenya, however, there are scarce data on the occurrence of the disease, as well as on the risk factors for transmission, in key production and consumption areas including Thika. The purpose of the study was to determine the seroprevalence of porcine cysticercosis in slaughtered pigs and associated risk factors for occurrence of the disease in selected abattoirs in Thika. Systematic random sampling was used to select a total of 276 pigs. The source of the slaughter pigs was derived from the movement permits, the breed, sex and estimation of age was done at ante mortem examination. The slaughter and meat inspection processes were carried out by the slaughter house personnel and the investigators only observed and received the outcome of the inspection. Blood samples were collected from each identified pig at slaughter, processed and analyzed using purified Taenia solium antigen ELISA commercial kit. The results meat inspection showed that none of the pigs in this study had any visible cysts whereas 4.35% of the pigs were seropositive which poses public health risk.
Prevalence of porcine cysticercosis in Vellore, South India
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2012
Background: Porcine cysticercosis is acquired by pigs through consumption of human faeces containing Taenia solium ova and indicates the presence of active transmission of the parasite between pigs and humans.