The Study of IBA, BAP and Kinetin Hormones Usage Towards The Buds Multiplications of Agarwood Plant (Gyrinops versteegii (Gilg) Domke) by In Vitro (original) (raw)

Agarwood is one of non timber forestry product which has a high economic value. Nowadays, the demand of agarwood is still depending on natural forest. The high demand which is followed by high persecution of agarwood in natural forest make the population of agarwood highly decreased. This research aimed to know the best combination of auxin (IBA) and cytokinin (BAP and kinetin) in basic medium of Murashige and Skoog towards the multiplications of Agarwood production plant explants by in vitro. Explants was grew in MS medium with the addition of growth hormone IBA (concentration of 0.00, 0.05, 0.10 mg/l), BAP (0.00, 0.10, 0.20 mg/l) and kinetin (0.00, 0.20, 0.40 mg/l). This research was arranged using the CRD factorial with 17 of treatments in 6 times repetition. The observed parameters such as the height growth, internodes total, buds total, total of leaves, the mortality percentage, callus production process, browning and contamination. The conclusion of this research was the effect of the addition of plant growth regulator combination of IBA (0.00, 0.05, 0.10 mg/l) with BAP (0.00, 0.10, 0.20 mg/l) and IBA (0.00, 0.05, 0.10 mg/l) with kinetin (0.00, 0.20, 0.40 mg/l) give the real response in parameter number of buds and leaves. Whereas on parameter like the height growth and internodes total give real and not different response.