Comparative Analysis of Hematological Parameters Determined By Cell-Dyn Ruby Automated Hematology Analyser and Manual Analysis (original) (raw)
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Annals of Tropical Medicine & Public Health, 2021
Background: Use of automated hematology analyzer in hematology laboratory is considered as a routine as well as an essential requirement in current scenario. The burden of anemia is high worldwide and there is increasing need to diagnose and treat this condition as it may lead to many complications on long run. Aim of the study: The present study was carried out to measure the sensitivity of red blood cell histogram and indices obtained through automated hematology analyzer and comparing it with manual blood film examination in patients of anemia. Material and methods: We have done this observational cross sectional study at Central hematology laboratory of Pathology department at tertiary care center in Gujarat. One thirty patients having anemia who have consulted different departments for variable reasons were included. Demographic and laboratory data were recorded. Analysis was done by Epi info software. Results: The study of included total 130 patients having anemia as per the WHO criteria for diagnosis of anemia. In our study we found sensitivity of histogram to be 87.6%. For microcytic hypochormic anemia, the sensitivity and specificity of red blood cell histogram and indices were 94.2% and 63.1%. For normocytic anemia, the sensitivity and specificity of red blood cell histogram and indices were 96.7%% and 49.1%. For macrocytic anemia, the sensitivity and specificity of red blood cell histogram and indices were 98.2% and 90.3%. Conclusion: The study showed that red blood cell histogram and indices obtained through automated
This study aimed to compare complete blood cell parameters obtained using the automated XN-1000 analyzer with those obtained by manual methods at the Kenyatta National Hospital hematology laboratory. About 110 samples were obtained from patients requesting complete blood count (CBC) test that were used in this study. Automated CBC test was done on Sysmex XN-100 hematology analyzer and validated against peripheral blood slide films read by a qualified lab technologist. Obtained data were analyzed using STATA v13 and the two methods compared using t-test and correlation. The study results showed a strong positive correlation between the automated and manual reader (p<0.001). However, basophil readings had a negative correlation for the two methods. A statistically significant difference was noted in neutrophil (p=0.013), monocyte (p<0.001) eosinophil (p=0.054) and MCHC (p<0.001) readings. The automated analyzer was unable to detect abnormal cell types. The study concluded that Peripheral blood film proved superior in detection of abnormal blood cells though the automated analyzer was able to handle a bigger workload and reduce the turnaround time. In view of the results, it is important to confirm results flagged by the automated analyzer using manual PBF.
Revista Brasileira de Hematologia e Hemoterapia, 2014
rev bras hematol hemoter. 2 0 1 4;3 6(3):219-225 Revista Brasileira de Hematologia e Hemoterapia Brazilian Journal of Hematology and Hemotherapy w w w . r b h h . o r g a b s t r a c t Objective: to verify whether the review criteria for automated blood counts suggested by Methods: initially, the review criteria of the International Society for Laboratory Hematology were adapted due to limitations in the Institution's electronic hospital records and interfacing systems. The adapted review criteria were tested using 1977 samples. After this first assessment, an additional 180 inpatient samples were analyzed to evaluate the screening criteria of the review criteria in conjunction with positive smear findings established by the institution. The performance of the review criteria was verified by determining false positive, false negative, true positive and true negative rates, sensitivity, specificity, positive predictive value, negative predictive value, microscopic review rate and efficiency.
American Journal of Clinical Pathology, 1992
The authors evaluated the performance of four modern, commercially available hematology analyzers for imprecision and inaccuracy in determining the leukocyte differential count. The evaluation was performed according to International Committee for Standardization in Haematology protocols and the National Committee for Clinical Laboratory Standards H20-T standard, using the same group of patients simultaneously. Imprecision was very low among all the analyzers for neutrophils and lymphocytes (coefficient of variation maximum = 4.12%), whereas for the other leukocyte populations it tended to increase as their presence percentage decreased. The imprecision of the analyzers was still lower than that of the microscopic method. The correlation with the manual 800 cell count (inaccuracy) was good for neutrophils, lymphocytes, and eosinophils (r = 0.974 to 0.888), less so for monocytes (r = 0.757 to 0.490), whereas it was poor for basophils (r = 0.532 to 0.078).
2014
Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematology testing in samples with ethylenediamine tetraacetic acid (EDTA). Objectives: Considering the importance of this hematology analyzer for clinical and laboratory practice, the objective of this study was to evaluate its analytical performance, comparing the obtained results with quality specifications described in literature. Material and method: In the evaluation of analytical performance, according to recommendations of the document H26-A2 of the Clinical and Laboratory Standards Institute (CLSI), intra-run imprecision, inter-run imprecision, linearity, carryover, autosampler evaluation, clinical sensitivity of the atypical lymphocytes flag (n = 400 samples) were included, as well as the comparison between automated and manual leukocyte differential count (n = 400 samples), based on an adaptation of the document H20-A2 of CLSI. Results: Repeatability, reproducibility, linearity and carryover were satisfactory according to the manufacturer's specifications. The clinical sensitivity of the atypical lymphocytes flag showed efficiency, sensitivity and specificity of 92.5%, 65.2% and 94.1% respectively. The correlation coefficients between the automated and manual differential counts of neutrophils, lymphocytes, monocytes, eosinophils and basophils were 0.991, 0.99, 0.872, 0.974 and 0.557, respectively. Conclusions: The results were in accordance with quality specifications described in literature, indicating reliability in Sysmex ® XE-2100D. This fact ensures certainty to both laboratory professionals and medical staff. We conclude that the Sysmex ® XE-2100D showed excellent analytical performance, and is useful to provide reliable hematology data.
Evaluation of the Abbott Cell-DYN 3500 hematology analyzer in university hospital
American journal of clinical pathology, 1996
The Abbott (R) Cell-Dyn 3500 (Abbott CD 3500, Abbott Diagnostics Division, Mountain View, CA) is a fully automated hematology analyzer capable of providing a complete blood count (CBC) profile, including a five-part differential leukocyte count (DLC) and flagging system in this study. The CBC profile and DLC flagging system of the Abbott CD 3500 were evaluated according to the HA-20 protocol of the National Committee for Clinical and Laboratory Standards (NCCLS) and compared to the Technicon H*2 blood analyzer currently used in the authors' laboratory. Linearity, carryover, precision, and stability were all within the limits established by the manufacturer. No significant break-downs were found during the evaluation period. Evaluation of DLC indicated an excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r = 0.916, 0.936, 0.967, respectively), a good correlation for monocytes (r = 0.811) and a poor correlation for basophils (r =...
International Journal of Current Research and Review, 2020
Objectives: Automated hematology analyzer generates flags for white blood cells abnormalities, indicating associated manual peripheral blood film examination. This study was aimed at evaluation and correlation of abnormal cell flagging with manual blood film reviews to improve performance in a hematology laboratory. In this study, an automated slide maker SC-120 was used to study the performance of fully automated haematology analyzer Mindray BC-6800. Methods: Total 500 routine samples were obtained over a period of seven consecutive days, and run through analyzer which displayed flags for white blood cells. Thereby, results from flags were evaluated and correlated with smear findings through careful statistical analysis of the observed parameters. Results: Flags for white blood cells and Nucleated Red Blood Cells (NRBC) were generated by BC-6800 for 211 samples out of total 500 run samples, from which 46.45% patients were diagnosed with haematological disorder and 53.55% with solid neoplasms. Sensitivity, specificity and Youden's Index observed against total 500 samples were 100%, 99.31% and 0.99 respectively. Efficiencies for blasts, abnormal lymphocytes and atypical lymphocytes were 95.64%, 95.20% and 97.60% respectively, whereas sensitivity for blasts was 75.81%. Further, sensitivity and specificity of NRBC were 86.76% and 100% respectively. Youden's index observed for various flags was close to 1, indicating near to satisfactory performance of analyzer. Conclusion: The present study confirms that performance of an automated analyzer aligns with standard manual methodology. However, to avoid false-negative results by analyzer, peripheral smears should be examined manually.
2015
Background: To assure the accuracy of results and performance of hematology analyzers, each laboratory must evaluate their performance. Different models of Sysmex and Cell-Dyn analyzers are commonly used in Ethiopia including St' Paul's Hospital Millennium Medical College. However, their performance has not been evaluated in most cases. If hematology analyzers are not verified and evaluated for the specific population they serve the diagnosis of many of diseases and hematology related clinical disorders will be unreliable. Objective: To evaluate the performance specifications of Cell-Dyn 1800 and Sysmex KX-21 that are found in St. Paul's Hospital Millennium Medical College. Methods: A hospital based cross-sectional study with purposive sampling technique was used at St. Paul's Hospital Millennium Medical College from April 1 to April 8; 2015. A sample of normal values was used for repeatability, high target values of hemoglobin, RBC, WBC and platelets for linearity and carryover checks with subsequent background count were used. Data were collected using observation checklist and then entry, cleaning and analysis were performed by Microsoft Excel 2007 and SPSS version 20. Percentage, %CV, residual plotting, and Pearson´s correlation coefficient were used to describe the results. Result: precision check revealed that both Sysmex KX-21 and Cell-Dyn 1800 values fall within the manufacturers' specifications. Linearity verifications of WBC, Hemoglobin, RBC and Platelet showed that both analyzers produced a very good correlation coefficient of > 0.97, which is also confirmed by residual plotting where all the values of the diluted samples surpassed the manufacturers' specifications. As to carryover both analyzers showed an excellent carryover percentage of <1% which surpassed the manufacturers' specifications. Conclusion: Sysmex KX-21 and Cell-Dyn 1800 met the manufacturer's specifications for precision, linearity and carryover.
Performance Comparison of Dymind DH-76 and Sysmex Xn-1000 Automated Hematology Analyzers
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY
An automatic hematology analyzer is an essential instrument for the modern laboratory. A new hematology analyzer must undergo comparability testing with a currently used hematology analyzer. This study aimed to compare the performance of the Dymind DH-76 and Sysmex XN-1000 hematology analyzers. This study involved 96 EDTA blood samples from patients aged 18 to 85 years old at a hospital in Jakarta. A complete blood count was performed on each blood sample using two above-mentioned analyzers Dymind DH-76 and Sysmex XN-1000. The results from both instruments were compared by using the Pearson correlation test and the Passing-Bablok regression analysis to determine the agreement in performance between the two instruments. Between the Dymind DH-76 and Sysmex XN-1000, there was a high degree of agreement and correlation concerning the investigated parameters with r > 0.900 and p<0.001 for the parameters RBC, HGB, WBC, HCT, MCV, MCH, and neutrophil, monocyte, and eosinophil counts. ...