Overexpression of native PSE1 and SOD1 in Saccharomyces cerevisiae improved heterologous cellulase production (original) (raw)

Heterologous expression of cellulase genes in natural Saccharomyces cerevisiae strains

Applied microbiology and biotechnology, 2016

Enzyme cost is a major impediment to second-generation (2G) cellulosic ethanol production. One strategy to reduce enzyme cost is to engineer enzyme production capacity in a fermentative microorganism to enable consolidated bio-processing (CBP). Ideally, a strain with a high secretory phenotype, high fermentative capacity as well as an innate robustness to bioethanol-specific stressors, including tolerance to products formed during pre-treatment and fermentation of lignocellulosic substrates should be used. Saccharomyces cerevisiae is a robust fermentative yeast but has limitations as a potential CBP host, such as low heterologous protein secretion titers. In this study, we evaluated natural S. cerevisiae isolate strains for superior secretion activity and other industrially relevant characteristics needed during the process of lignocellulosic ethanol production. Individual cellulases namely Saccharomycopsis fibuligera Cel3A (β-glucosidase), Talaromyces emersonii Cel7A (cellobiohydro...

Recombinant cellulase expression in Saccharomyces cerevisiae

2013

The non-renewable fossil resources currently exploited by the oil and gas industries are the objects of growing concern owing to their finite supply and contribution to global warming. Lignocellulosic biomass is a sustainable alternative to fossil resources, and has the added advantage of not competing with human and animal nutrition. Indeed, lignocellulosic biomass, in particular its main polymer component cellulose, is a potential carbon source for the production of fuels and commodity chemicals in microbes.