Lipid Vesicle Detection using ISFET devices (original) (raw)
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Lipid Vesicle Detection using ISFET devices Detección de vesículas lipídicas con ISFETS
2013
The Ion Sensitive Field Effect (ISFET) Transistors are electronic devices widely used for detecting, ion concentrations, trapped charge or charge decay processes. This article reports on the electrical characterization of ISFETs exposed to buffer solutions sets at pH 4, 5, 7, and 10. When operated in their saturation regime ISFETs act as local charge detectors. This capability is applied to detect lipid vesicles. Lipid vesicles have promising applications in drug delivery, and their accurate detection can be important in understanding their interaction with living systems.
Biosensors and Bioelectronics, 2000
The pH in the cellular microenvironment (pH(M)) is an important regulator of cell-to-cell and cell-to-host interactions. Additionally the extracellular acidification rate of a cell culture is an important indicator of global cellular metabolism. In a new approach a biocompatible ion-sensitive field effect transistor (ISFET)-array was developed to measure the pH(M) close to a surface and the global extracellular acidification rate at the same time. This ISFET-array is part of a new multiparametric microsensor chip. The paper highlights some basic applications of this method for in-vitro measurements. Using a fluid perfusion system for cell culture media, it is possible to measure the pH(M) of few (five to ten) adherent tumor cells in a distance of 10-100 nm from the cell plasma membrane. Experiments showed a pH(M)-value of 6.68 +/- 0.06 pH. Further experiments suggest that both the low pH, and the extracellular acidification rate of the examined tumor cell line are mainly built up by glycolysis.
MODELING ION SENSITIVE FIELD EFFECT TRANSISTORS FOR BIOSENSOR APPLICATIONS
iaeme
During recent decades increasing interest has been shown in the development of biosensors based on ion sensitive field effect transistors (ISFETs). Many ISFET– based pH sensors have been already commercialized and attempts have also been made to commercialize ISFET- based biosensors for applications in the fields of medical, environmental, food safety, military and biotechnology areas. The growing interest for development of these sensors can be explained by the fact that they are manufactured by means of semiconductor techniques which have innovative potential and therefore, may result in the appearance of new biosensor technologies. The basic theoretical principles of ISFET usage in bio analytical practice, the operation principle of ISFET, its modeling and a brief introduction of ISFET technology are considered in this review.
Proton-ELISA: Electrochemical immunoassay on a dual-gated ISFET array
Biosensors & bioelectronics, 2018
Here we report an electrochemical immunoassay platform called Proton-ELISA (H-ELISA) for the detection of bioanalytes. H-ELISA uniquely utilizes protons as an immunoassay detection medium, generated by the enzyme glucose oxidase (GOx) coupled with Fenton's reagent in a proton amplification reaction cascade that results in a highly amplified signal. A proton-sensitive dual-gated ion-sensitive field effect transistor (DG-ISFET) sensor was also developed for sensitive and accurate detection of the proton signal in H-ELISA. The DG-ISFET sensor comprises of a 128 × 128 array of 16,384 sensing transistors each with an individually addressable back gate to allow for a very high signal throughput and improved accuracy. We then demonstrated that the platform could detect C-reactive protein and immunoglobulin E down to concentrations of 12.5 and 125 pg/mL, respectively. We further showed that the platform is compatible with complex biological sample conditions such as human serum, suggest...