Assessment of genetic diversity among sunflower genotypes using microsatellite markers (original) (raw)
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MICROSATELLITE DNA MARKER AIDED DIVERSITY ANALYSIS IN CONFECTIONERY SUNFLOWER (HELIANTHUS ANNUUS L
Genetic diversity among 48 genotypes of confectionery sunflower (Helianthus annuus L.) collected from USDA, USA and India were evaluated by using microsatellite SSR markers. Twenty five sunflower specific SSR primers were used. Of the 25 SSR primers used, 10 primer pairs () showed polymorphism. The high level of polymorphism (66.66%) was reported in this finding and the number of alleles in SSR loci ranged from 2 to 4 with an average of 2.5. The present study identifies the promising lines EC 734807, EC 734808,EC 734810,EC734860 and EC 734817 for protein and yield, these can be used as potential donors in future hybridization programme.
STUDY ON GENETIC DIVERSITY IN SUNFLOWER (HELIANTHUS ANNUUS L.) 33 ELITE GENOTYPES
2024
In the present investigation, thirty three sunflower (Helianthus annuus L.) genotypes including three checks were evaluated to study genetic divergence. The experiment was laid out at Research Farm, School of Agriculture, ITM University, Gwalior during rabi 2020-2022. Data were recorded on ten quantitative characters. The thirty three genotypes of sunflower were grouped into seven cluster using Tocher method of the seven clusters formed, cluster I and III were the largest groups comprising of eleven and eight genotypes, respectively, followed by cluster V with six genotypes, cluster II with five genotypes. The 3 monogenic clusters i.e. IV, VI and VII showed zero intra-cluster. Based on cluster mean values for a given character, we can select highly divergent genotypes from the respective clusters to be used in crossing work.
African Journal of …, 2010
Information about the genetic diversity and relationships among breeding lines and varieties is not only useful for germplasm conservation and inbred line identification, but also for the selection of parental lines for quantitative trait loci (QTL) mapping as well as hybrid breeding in crops, including sunflower. In order to develop mapping populations, genetic distances among twenty eight sunflower genotypes were evaluated using simple sequence repeat (SSR) markers. One hundred and two markers were generated by 38 SSR loci and the mean for the number of allele per locus was 2.32. Polymorphism information content (PIC) values ranged from 0.09 (locus ha3555) to 0.62 (locus ORS598) with an average of 0.41. Jaccard's coefficient similarity matrix for the studied sunflower genotypes varied from 0.25 to 0.9 indicating a broad genetic base. The maximum similarity (0.9) was observed between genotypes RT931 and ENSAT-R5, while the lowest similarity (0.25) was between genotypes LC1064C and LR64. Based on unweighted pair group method with arithmetic mean (UPGMA) clustering algorithm, the studied genotypes were clustered in four groups. However, some genotypes have the same specific characters that influence their clustering, and as a result, the results of the principal coordinate analysis (PCoA) largely corresponded to those obtained through cluster analysis.
Genetics and Molecular Research, 2008
The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li's similarity matrix gave values from 51.59 to 77.78%, which indicated a broad genetic base. The maximum similarity, 77.78%, was observed between R-SIN-82 and RN-46. The lowest similarity, 51.59%, was observed between the exotic lines CM-612 and HA-27. After knowing the knowledge of genetic diversity based on these random amplified polymorphic DNA markers, highly diverse lines can be used for further breeding programs to develop an ideal local hybrid of sunflower.
Assessment of Genetic Diversity in Sunflower (Helianthus annuus L.) Germplasm
International Journal of Agriculture and Food Science Technology, 2014
The, present, investigation,was,carried,out,at,the, research, fields,of, the, oilseeds,Sections,Department,of,Plant,Breeding,and,Geneticss,Punjab, Agricultural, Universitys, Ludhianas, India:, The, material, for, present, study,consisted,of,Dj,germplasm,lines:,The,material,was,raised,in,two, rows,with,three,replications,each,row,of,B:Gm, length,with,Uw,cm,and, Dw, cm, inter, and, intra, row, spacing, respectivelys, in, the, randomized, block,design:,All,the,agronomic,practices,recommended,for,the,region, were,followed,to,raise,a,good,crop:,The,data,for,agronomic,and,yield, traits,i:e:,plant,heights,head,diameters,volume,weights,jww,seed,weights, seed, yield, per, plant, was, recorded:, Dv analysis, assigned, the, test, genotypes, into, six, clusters, indicating, presence, of, enough, genetic, diversity,in,the,material
Microsatellite isolation and characterization in sunflower ( Helianthus annuus L.)
Genome, 2002
Development of microsatellite markers for sunflower (Helianthus annuus L.) was performed to estimate their frequency, nature (structure), levels of polymorphism, usefulness for genotype identification, and calculation of genetic relationships between inbred lines representing the species diversity. Isolation was performed from a small-insert genomic library followed by hybridization screening using oligonucleotide probes containing different nucleotide arrays. In this work, 503 unique microsatellite clones were sequenced and 271 PCR primer sequences bordering the microsatellite repeat were designed. For polymorphism assessment, 16 H. annuus germplasm accessions were checked and 170 of the primers tested were shown to be polymorphic for the selected lines. The polymorphic microsatellites produced an average of 3.5 alleles/locus and an average polymorphism information content (PIC) of 0.55. The most frequently found motifs within polymorphic simple-sequence repeats (SSRs) were: (GA) n , (GT) n , (AT) n , followed by trinucleotides (ATT) n , (TGG) n, and (ATC) n , and the tetranucleotide (CATA) n . Most of the 170 SSRs obtained showed important differences in the 16 reference inbred lines used for their characterization. In this work, 20 of the most informative SSRs destined to sunflower genotyping and legal fingerprinting purposes are fully described.
2008
This study compared DNA based SSR markers with total seed protein markers, used to evaluate genetic diversity of sunflower. The multiplex-ability, cost effectiveness and applicability of microsatellites as molecular markers for a genetic diversity study were investigated and evaluated based on pedigree data of the sunflower germplasm. A solution for oil and fat interference in ultrathin iso-electric focusing gels was investigated, in order to make imaging and interpretation easier and clearer. Total protein analysis was utilized for the determination of genetic diversity on the same inbred material used for the DNA analysis. Finally a correlation is made between the data obtained on DNA vs Protein compared with phenotype and expected pedigree data. A set of 73 SSR markers with known mapped positions were utilized to determine genetic similarity in a group of sunflower inbred lines. Cluster analysis of genetic similarity revealed an excellent correlation with the breeding background ...
Genetic variability and genetic diversity in sunflower
Electronic Journal of Plant Breeding
One hundred and seventy four accessions of sunflower were evaluated in a randomized block design with two replications during Kharif 2010-11 at Regional Agricultural Research Station, Nandyal, Andhra Pradesh to investigate the extent of genetic variability and genetic diversity. The Analysis of variance revealed significant differences among the genotypes for all the traits indicating considerable magnitude of genetic variability of the material used for the study. Low heritability coupled with high GAM was observed for seed yield per plant due to high environmental effects and selection is effective. The genetic divergence study infers the grouping of 174 accessions in 13 clusters which indicates prevalence of good extent of diversity in the material. Cluster I had more no. of genotypes followed by clusters IV, VII and VIII. Cluster X had higher mean value for plant height and head diameter, cluster XII had higher test weight and cluster XI had high seed yield per plant. The maximum inter cluster distance was recorded between cluster XI and XII followed by cluster X and XII and clusters V and XII. Hence, it is suggested that if the diverse accessions from these diverse groups are used in the breeding programme, it is expected to produce a wide range of genetic variability in the population. The traits, 100 seed weight, plant height and days to maturity were the major causing genetic divergence among the accessions.
Journal of Agricultural Sciences, Belgrade, 2009
The objective of this study was to determine the correlation between SSR based genetic distance (GD) of new NS sunflower inbred lines and heterosis for six agronomic traits. Twenty three sunflower inbred lines (20 restorer lines and three female lines used as testers) and their hybrids were examined for plant height, head diameter, thousand seed mass, oil content, seed yield per plant and oil yield per plant. Significant heterosis was observed in hybrid combinations for all examined traits except oil content. Genetic distance between pairs of tested sunflower inbred lines ranged from 0.13 to 0.8. There was no significant positive correlation between genetic distance and mid- and better-parent heterosis, specific combining ability and mean value in any of the examined traits for all 60 hybrids. A highly significant negative correlation was found between GD and mean oil percentage (r=-0.33 p<0.01). Although GD was generally a poor predictor of heterosis, better results are obtained...