Nitrogen Mobilization and Proteolytic Activities in Germinating and Maturing Bush Beans (Phaseolus vulgarisPhaseolus vulgaris L.) (original) (raw)

Abstract

Changes in the nitrogen contents and in the extractable actlVltIes of amino-, carboxyand endopeptidases were analyzed in bush beans (Phaseolus vulgaris 1.) throughout the life cycle. Two types of casein digesting enzymes (endopeptidases) were found. One type was the main activity extracted from cotyledons during germination with a pH optimum around pH 5.5 and was stimulated by the presence of mercaptoethanol in the incubation medium. The other type showed high activities in leaf extracts, was most active around pH 7 and was slightly inhibited by the presence of 0.1 Ofo mercaptoethanol in the incubation medium.

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