SCREENING OF SOME BIOLOGICAL ACTIVITIES OF ALYSSUM FULVESCENS VAR. FULVESCENS KNOWN AS EGE MADWORT (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: To evaluate the antimicrobial activity and cytotoxicity of hexane, ethyl acetate and methanol extracts of Alysicarpus vaginalis var. nummularifolius (DC) along with phytochemical analysis. Methods: The crude extract of hexane (AVH), ethyl acetate (AVE) and methanol (AVM) of Alysicarpus vaginalis var. nummularifolius (DC) were prepared and analysed for phytochemical constituents using standard methods. The cytotoxicity activity of the plant extracts was predicted using brine shrimp lethality assay (BSLA). The antimicrobial activity and the minimal inhibitory concentration (MIC) of the plant extracts were examined against 5 bacterial and 2 fungal strains using agar well diffusion method, and two fold serial dilution method, respectively. Results: The phytochemical screening studies showed a higher concentration of saponins, alkaloids, flavonoids, phenols, quinones and terpenoids in AVM than the other two extracts. The LC50 value of AVH and AVE were found to be 900.05 µg/ml and 754.35 µg/ml respectively using BSLA while that of AVM was>1000 ug/ml. All the extracts of the plant showed antimicrobial activity against most of the test organisms. The MIC values of AVM were lower than AVE for all the microbial strains except for Pseudomonas aeroginosa where AVE (107.87µg/ml) exhibited higher value than AVM (51µg/ml). Conclusion: The present study concluded AVM with a high presence of phytochemicals. The AVE and AVM were found to possess promising antimicrobial activity when compared with the standards. The AVM exhibited lesser toxicity when compared with AVH and AVE.
2010
Alnus glutinosa, Fraxinus excelsior and Papaver rhoeas have long been used in folkloric medicine for the treatment of various ailments. As part of our continuing screening of plant extracts for biological activities, the extracts of A. glutinosa, F. excelsior and P. rhoeas have been screened for their antioxidant and antibacterial activities, as well as their general toxicity towards brine shrimps. Among the extracts, the methanol (MeOH) extract of F. excelsior displayed the highest level of antioxidant activity (RC 50 =1.35x10-2 mg/mL) and the dichloromethane (DCM) extract of P. rhoeas was the most toxic extract towards brine shrimps (LD 50 =2.4x10-2 mg/mL). The n-hexane and DCM extracts of F. excelsior and the MeOH extract of A. glutinosa were active (MIC values were within 1.25x10-1 and 1.00 mg/mL) against all 8 bacterial species tested, including methicillin-resistant Staphylococcus aureus (MRSA).
Antioxidant, Antibacterial Activities and General Toxicity of Alnus
2016
ch ive of SIDAbstractAlnus glutinosa, Fraxinus excelsior and Papaver rhoeas have long been used in folkloric medicine for the treatment of various ailments. As part of our continuing screening of plant extracts for biological activities, the extracts of A. glutinosa, F. excelsior and P. rhoeas have been screened for their antioxidant and antibacterial activities, as well as their general toxicity towards brine shrimps. Among the extracts, the methanol (MeOH) extract of F. excelsior displayed the highest level of antioxidant activity (RC50=1.35x10-2 mg/mL) and the dichloromethane (DCM) extract of P. rhoeas was the most toxic extract towards brine shrimps (LD50=2.4x10-2 mg/mL). The n-hexane and DCM extracts of F. excelsior and the MeOH extract of A. glutinosa were active (MIC values were within 1.25x10-1 and 1.00 mg/mL) against all 8 bacterial species tested, including methicillin-resistant Staphylococcus aureus (MRSA).
Bangladesh Journal of Pharmacology
The methanolic extract of Alysicarpus vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for in vitro antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From the all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC50 values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and E. coli whereas chloroform and n-butanol fractions were more effective against S. aureus and B. subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.
Phytochemical profiling of the bioactive principles of Alysicarpus glumaceus (Vahl) DC. aerial parts
2021
Background and Aims: Alysicarpus glumaceus is a plant used in Africa, Asia and some parts of the Arabian Peninsula with folkloric claims of having anti-tussive, anti-asthmatic, anti-diarrheal, abortifacient, anti-psychotic, anti-inflammatory, diuretic and stimulatory activities. The aim of this study was to determine the phytoconstituents present in the methanol extract (ME) of Alysicarpus glumaceus and its fractions. Methods: Standard qualitative phytochemical screening methods such as thin layer chromatography (TLC), gas chromatography mass spectrometry (GC-MS) and fourier-transform infrared (FT-IR) spectroscopy were employed for the profiling of the plant and identification of the phytoconstituents. Results: The phytochemical screening revealed the presence of alkaloids, cardiac glycosides, flavonoids, saponins, steroids, tannins and terpenes. GC-MS chromatogram showed a total of 57 peaks with 38 different compounds identified, out of which 15 of the compounds were fatty acids ma...
Bangladesh Journal of Pharmacology, 2017
The methanolic extract of Alysicarpus vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for in vitro antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC50 values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and E. coli whereas chlorofo...
Basic Research Journal of Microbiology, 2019
This study determined the total phenols and flavonoids, antioxidant activity, Preliminary Phytochemical Screening, GC-MS analysis. Hypnea boergesenii T. Tanakared seaweed, collected from Chabahar Sea, on the coast of the Gulf of Oman. Powder was extracted using five solvent: (15g) with70% ethanol and (340g) extracted successively with four solvent (Hexane, petroleum ether, dichloromethane and 1445 methanol) using maceration method. All the crude extracts were concentrated under reduced pressure and dried by using freeze dryer. The antioxidant activity of the methanolic extract was measured using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and the IC 50 is (17.68± 0.33). The total phenolics and total flavonoids content were also determined by Folin-Ciocalteu reagent and aluminum chloride colorimetric methods (TPC =3.42±0.04) and (TFC = 4.79 ± 0.11). In this investigation, was performed to screen, estimate total phenolic, flavonoids, evaluate antioxidant potential of H. boergesenii and investigate Antimicrobial activity of the seaweed extracts. Phytochemical analysis of alcoholic extract showed presence of major classes of phytochemicals, such as: tannins, flavonoids, cardiac glycosides, steroids, etc. Gas chromatography-mass spectrometry results revealed presence phytoconstituents compounds in various extract of H. boergesenii alga.
Journal of Pharmacognosy and Phytochemistry, 2019
Alchornea cordifolia has ethnomedicinal use in the treatment of wounds, gonorrhea, conjunctivitis, diarrhea, urinary disorder and gastrointestinal disorder. The main focus of this study was to verify the biological activities (anthelmintic, antimicrobial and antioxidant) and isolate some of the components in the chloroform, methanol, and petroleum ether leaf extracts. Phytochemical screening, antimicrobial (agar and broth dilution method), anthelminthic and antioxidant [total antioxidant capacity (TAC), DPPH and H2O2 scavenging] assays were carried out on extracts. Thin layer and column chromatography were employed in the isolation and purification of the components of the petroleum ether extract. The phytochemical investigation revealed the presence of secondary metabolites such as terpenoids, flavonoids, steroids, saponins, carotenoids, phenols and tannins. All three extracts showed antimicrobial activity against test organisms with MICs ranging from 1.56 – 25 mg/mL. The IC50 valu...