A REVIEW ON GREEN SYNTHESIS OF SILVER NANOPARTICLE USING FRUITS EXTRACT (original) (raw)
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A REVIEW ON GREEN SYNTHESIS OF SILVER NANOPARTICLES USING FRUITS EXTRACT
Silver is nontoxic, safe inorganic agent used for management of wounds and ulcer. It has wide range of application in form of nanoparticles. Synthesis of silver nanoparticles through cost effective and environmental friendly method is of great concern. This review focuses on green synthesis of silver nanoparticles from fruits. A detailed study of synthesis of silver nanoparticles from various fruit extracts was demonstrated. Synthesis was observed as a colour change of the mixture. The X-ray diffraction patterns, UV-visible spectrum, scanning electron microscope, transmission electron microscope, fourier transform infrared spectroscopy were used for characterization of Silver nanoparticles. Antibacterial activitiy of Silver nanoparticles was observed against Gram-positive and Gram-negative bacteria. Various informations on synthesis, characterization techniques, properties and application of Silver nanoparticles are summarised and critically discussed in this review.
A preliminary study on green synthesis and antibacterial activity of silver nanoparticles
2015
Silver nanoparticles have attracted increasing interest due to their unique physical, chemical and biological properties compared to their macro-scaled counterparts. A comparative study of chemical and biological method for Silver nanoparticle synthesis is done. Few locally available fruits including Lemon (Citrus aurantifolia), Orange (Citrus sinensis) and Tomato (Solanum lycopersicum), which are known to contain relatively high amount of ascorbic acid were selected for silver nanoparticle synthesis. Silver nanoparticles were prepared from aqueous solution of silver nitrate using different fruit extracts. The synthesized silver nanoparticles have been characterized by UV-visible spectroscopy. The UV-Vis spectrophotometer analysis showed a peak for silver nanoparticles between 2-6 hours for the samples kept at room temperature (37ºC). It was also observed that orange extract synthesized nanoparticles showed highest peak, indicating synthesis of maximum nanoparticles and Trisodium citrate synthesized nanoparticles showed the minimum. Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method. Antibacterial activity of the synthesized silver nanoparticles was tested with one Gram-positive (Staphylococcus aureus) and one Gram-negative (Escherichia coli) bacterium. Antibacterial effect was found to be maximum for lemon extract synthesized nanoparticles, and it was found that the silver nanoparticles have antibacterial activity against Escherichia coli and Staphylococcus aureus.
Journal of Nanomedicine & Nanotechnology, 2015
Synthesis of nanosized particles with antimicrobial property is of significance in therapeutic applications of nanotechnology. The present study reports an environmental friendly and rapid method for biosynthesis of silver nanoparticles and their antibacterial activity. This green synthetic method used various fruit juices like sweet lime, lime and orange as a reducing and capping agent for silver nitrate. Different thermal reduction methods-microwave oven and hot plate were used for synthesizing silver nanoparticles. The silver ions were reduced into silver nanoparticles within few minutes of reaction. Silver nanoparticles so prepared were characterized using UV-visible spectrophotometer and scanning electron microscope. An effort was also been made to predict the size of the silver nanoparticles using UV-visible spectra by Mie Scattering protocol. Scanning electron micrograph (SEM) revealed useful information about the morphology of silver nanoparticles. The synthesized silver nanoparticles showed antibacterial property against pathogenic bacteria Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Staphylococcus aureus. Agar well diffusion method was adopted to assay the nanoparticles for bactericidal activity against test organisms on nutrient agar plates. Silver nanoparticles biosynthesized from orange fruit juice were more effective as antibacterial agent. The maximum zones of inhibition of 8.0 mm, 6.0 mm, 8.0 mm and 5.0 mm were observed against Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Staphylococcus aureus respectively. The study shows that gram negative bacteria are more susceptible to antibacterial action of silver nanoparticles. Such studies are crucial in the demonstration of therapeutic importance of silver nanoparticles.
Zenodo (CERN European Organization for Nuclear Research), 2020
Silver nanoparticles (AgNPs) are gaining attention because of its wide application in medical science as antimicrobial and antibacterial properties. A conventional method for synthesis of AgNPs consumes hazardous chemicals and during production of NPs harmful by-products may also form. Proposed review suggests "greener technology" for synthesis of silver nanoparticles with the help of easily available plants. Aqueous extract of leaf or roots of Impatiens balsamina, Lantana camara, Eriobotrya japonica, Berberis vulgaris, Azadirachta indica, Psidium guajava, Moringa oleifera and Catharanthus roseus and other plant extracts are used as reducing agents to convert Ag + into Ag 0. Light or faint color of plant extract after addition of AgNO 3 turns to brown or deep brown in color, indicates the formation of AgNPs. Fourier Transform Infra Red Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Tunneling Electron Microscopy (TEM) and Dynamic Light Scattering Spectroscopy (DLSS) are techniques employed for characterization and morphological analysis of synthesized silver nanoparticles. Antibacterial, antifungal and antiparacitic applications of synthesized AgNPs have been also discussed. It is concluded that AgNPs synthesized by green way has great potency towards both Gram-negative and Gram-positive bacteria. Moreover, green synthesis of silver nanoparticles is a nonhazardous, eco-friendly, economic and safe technique and will envisaged better option for synthesis of AgNPs.
Advances in Nanoparticles, 2024
Green synthesis of silver nanoparticles (AgNPs) using aqueous extracts of orange and lemon peels, as a reducing agent, and silver nitrate salts as a source of silver ions is a promising field of research due to the versatility of biomedical applications of metal nanoparticles. In this paper, AgNPs were synthetized at different reaction parameters such as the type and concentration of the extracts, metal salt concentration, temperature, speed stirring, and pH. The antibacterial properties of the obtained silver nanoparticles against E. coli, as well as the physical and chemical characteristics of the synthesized silver nanoparticles, were investigated. UV-Vis spectroscopy was used to confirm the formation of AgNPs. In addition to green biogenic synthesis, chemical synthesis of silver nanoparticles was also carried out. The optimal temperature for extraction was 65˚C, while for the synthesis of AgNPs was 35˚C. The synthesis is carried out in an acidic environment (pH = 4.7 orange and pH = 3.8 lemon), neutral (pH = 7) and alkaline (pH = 10), then for different concentrations of silver nitrate solution (0.5 mM-1 mM), optimal time duration of the reaction was 60 min and optimal stirring speed rotation was 250 rpm on the magnetic stirrer. The physical properties of the synthesized silver nanoparticles (conductivity, density and refractive index) were also studied, and the passage of laser light through the obtained solution and distilled water was compared. Positive inhibitory effect on the growth of new Escherichia coli colonies have shown AgNPs synthesized at a basic pH value and at a 0.1 mM AgNO 3 using orange or lemon peel extract, while for a 0.5 mM AgNO 3 How to cite this paper: Bratovcic, A. and Dautovic, A. (2024) Green Synthesis of Silver Nanoparticles Using Aqueous Orange and Lemon Peel Extract and Evaluation of Their Antimicrobial Properties. Advances in Nanoparticles, 13, 11-28.
Green Synthesis and Antibacterial Activities of Silver Nanoparticles
2012
Silver ions and silver compounds have been known to have strong antibacterial activities. Using silver nanoparticles lead to an increase in number of particles per unit area thus, antibacterial effects can be maximized. In this study, silver nanoparticles were prepared by treating a solution of AgNO3 dissolved in deionized water with Vitamin C rich fruits (Guava, grape and tomato) as reducing agent. The factors that affect the synthesis of silver nanoparticles (reaction time, temperature and concentration of silver nitrate) were investigated. The nanoparticles were characterized by UV-vis spectrophotometer, X-ray diffraction and Fourier transform infrared (FT-IR). A suitable condition for silver nanoparticle synthesis is using 0.001M silver nitrate and guava extract at 60 °C for 8 hours. The UV-vis spectrophotometer show peak located of silver nanoparticles at 420 nm. X-ray diffraction analysis also showed functional structure and pattern of silver nanoparticles. The FT-IR spectra i...
The synthesis of metal nanoparticles is a growing area for research due to its potentiality in the application and development of advanced technologies. In general, nanoparticles are synthesized by using chemical methods which are not eco-friendly. Here, we have used a fast, convenient and environment-friendly method for the synthesis of silver nanoparticles by reducing silver nitrate with fruit extract of grape (Vitis vinifera). Characterization of the metallic nanoparticles was done by UV-Vis Spectroscopy, Dynamic Light Scattering (DLS) and Energy Dispersive X-ray Spectroscopy (EDX). The particle size and lattice image of the silver nanoparticles was studied by Transmission Electron Microscopy (TEM). The antibacterial activity of these nanoparticles was studied against Bacillus subtilis and Escherichia coli. Growth curves of bacteria in presence of silver nanoparticles showed inhibition of growth suggesting antibacterial property of the nanoparticles.
Energy Procedia, 2016
A facile, green and eco-friendly approach was introduced for the synthesis of silver nanoparticles using dragon fruit peel extract (DPE) as reducing as well as capping agent. The effect of pH on the formation of silver nanoparticles was studied utilizing various characterization techniques including UV-Vis spectroscopy, FTIR, XRD, EDX, TEM and zeta potential. The formation of silver nanoparticles was confirmed by UV-Vis spectroscopy with the presence of surface plasmon resonance band between 430 and 460 nm. TEM images demonstrated that the size of synthesized silver nanoparticles was reduced with an increase of pH. The silver nanoparticles was spherical in shape with a negative charge on the surface of the nanoparticles. The size of the nanoparticles was in the range of 25-26 nm. XRD and EDX analysis indicated that the biosynthesized nanoparticles were crystallized in face centered cubic symmetry with the existence of Ag 2 O. A possible organic compound in DPE involving in the reduction and stabilization of nanoparticles was revealed by FTIR. Moreover, the synthesized nanoparticles exhibited effective antibacterial action against representative pathogenic bacteria.
Green Synthesis of Silver Nanoparticles Using Apple Extract and Its Antibacterial Properties
Advances in Materials Science and Engineering, 2016
Silver nanoparticles (AgNPs) were synthesized using apple extract as a reducing agent and aqueous silver nitrate as the precursor. The AgNPs formation was observed as a color change of the mixture from colorless to dark-brownish. The X-ray diffraction pattern confirmed the presence of only Ag crystallites, and the dynamic light scattering estimates the average sizes of the AgNPs to be 30.25 ± 5.26 nm. Furthermore, Fourier Transform Infrared as well as UV-vis spectroscopy identifies ethylene groups as the reducing agent and capping agent for the formation of the AgNPs. This green synthesis provides an economic, eco-friendly, and clean synthesis route to AgNPs. AgNPs in suspension showed activity against Gram-negative and Gram-positive bacteria with minimum bactericidal concentrations (MBCs) to be in the range from 125 g/mL to 1000 g/mL.
Evaluation of antibacterial activites of silver nanoparticles green-synthesised using pineapple leaf
Pineapple leaf was used in this study for the synthesis of silver nanoparticles based on the search for sustainable synthetic means. Indeed, this offered an economical and sustainable synthetic route relative to expensive and toxic chemical methods. The leaf extract was used and the corresponding nanoparticles obtained were subjected to UV-vis analysis at different times. The UV-vis was used to monitor the silver nanoparticle formation through sampling at time intervals. The formation of silver nanoparticles was apparently displayed within 2 min with evidence of surface plasmon bands (SPB) between 440 and 460 nm. The crystals was equally characterized using FTIR, X-ray diffraction methods and TEM. The different results obtained suggested the appearance of silver nanoparticles (SNPs) as determined by the process parameters with a particle size of 12.4 nm. The sample was further screened against Staphylococcus aureus, Streptococcus pneumoniae, Proteus mirabilis and Escherichia coli using Gentamicin as control. From the results, there is evidence of inhibition towards bacteria growth. It can now be inferred from the studies that biosynthesis of nanoparticles could be a gateway to our numerous health issues.