Buffers for Reversed-Phase Liquid Chromatography (original) (raw)

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, 2015

Abstract

The parameters affecting the properties of buffers commonly used in chromatographic mobile phases, mainly the content of organic solvent and temperature, are examined. They play a key role in the pH of maximum buffer capacity and the effective pH range of buffering action. The effect of organic modifier on the pKa of buffering acids and bases and, consequently, on the pH is discussed, and the relation of this pH variation with the retention of analytes with acid–base properties is pointed out. The different pH scales used in hydroorganic mobile phases and their relationships are discussed as well.

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