Determination of Antibacterial Efficacy of AgNPs Synthesized by Leaf extract of Azadirachta indica against Staphylococcus aureas and Bacillus cereus (original) (raw)

2018, ,3rd International Conference on Communication and Electronics Systems (ICCES 2018) IEEE sponsored, Coimbatore, India, October 2018.

Abstract

Green synthesis of silver nanoparticles using leaf extract of Azadirachta indica (Neem) for development for antibacterial agent. 0.01 M Silver nitrate (100 mL) was mixed with 3 mL crude extract of leaf and incubated resultant reaction mixture in ice bath for 45 min. The reddish brown color was observed after incubation, which was characterized by UV-Vis Spectroscopy and Scanning Electron Microscopy (SEM). The λmax was found to be in the range of 201 nm-207 nm and optical band gap calculated was 5.59 eV to 6.17eV. Antibacterial efficacy was determined by anti-well diffusion assay against Staphylococcus aureus and Bacillus cereus. Both the cultures were found to be sensitive for Ag NPs at concentration range of 10 µg/mL-1000 µg/mL. The Bacillus cereus was more sensitive for Ag NPs compared to Staphylococcus aureus.

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