Agro-industrial residues as alternative sources for cellulases and xylanases production and purification of xylanase produced by Aspergillus flavus AUMC 10331 isolated from extreme habitat (original) (raw)

Agro-industrial residues, namely wheat bran (WB), red sawdust (RSD), white sawdust (WSD), corn cobs (CC), rice husk (RH) and sugarcane bagasse (SB) were used as alternatives of pure xylan for production of xylanase, carboxy methyl cellulase (CMCase) and avicellase in solid state (SSF) and submerged (SmF) fermentation using Aspergillus flavus AUMC 10331. In SSF, WB was the most utilizable yielding the highest concentration of xylanase (792.4 IU.gds-1) and CMCase (101.8 IU.gds-1) and RH was the best for avicellase (152.4 IU.gds-1). In SmF, A. flavus regularly utilized all substrates to produce the three enzymes. From the six plant residues, CC was the superior source for xylanase production (9660 IU.gds-1) and CMCase production (307 IU.gds-1), while RH registered the highest avicellase (5554 IU.gds-1). Xylanase from CC was purified using ion exchange (IR-120 EP) and Sephadex G-75 column chromatography. The purified xylanase showed activity of 32.77 IU.ml-1 .min-1 and a specific activity of 273.1 IU.mg-1 proteins. The enzyme was active over a pH range of 4.5-8.0, and its highest activity was detected at pH 7.0 and 65 ºC. Xylanase activity was stimulated by FeSO4 up to 121.97 %. Km and Vmax were 12.18 mg.ml-1 and 204.1 IU.min-1 , respectively.

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