Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.) (original) (raw)
Related papers
2016
Herbicide resistant (HR) rice is a novel approach to enhance selectivity and crop safety in rice cultivation. HR crops provide better suppleness in weed management and new solutions to difficult weed management problems. Studies on induced HR in Sri Lankan rice varieties are limited and further research are required to include HR rice in a cropping program. Mutational breeding with molecular methods play an important role in resistant development in modern agriculture. The present study is an attempt to develop HR rice lines through conventional breeding methods using the chemical mutagen EMS. A detailed AFLP analysis was made to identify molecular markers for HR induced varieties. Twenty five cultivated rice varieties were subjected to EMS treatment and after the mutation survived plants were exposed to glyphosate to check herbicide resistance. AFLP analysis was made on EMS mutated rice plants using 16 AFLP primer combinations. Seed-derived calli were obtained from glyphosate-susce...
Inheritance of herbicide resistance in two germplasm lines of Clearfield* rice (Oryza sativa L.)
Canadian Journal of Plant Science, 2007
Inheritance of imidazolinone resistance in two germplasms of Clearfield rice lines, 93AS3510 and PWC-16, was studied using parents, F1 hybrids, F2 populations , and F2:3 families. Germination tests were conducted in Petri dishes under controlled environments to reveal any discrete phenotypic responses to herbicide treatments. PWC-16 has a herbicide resistance level 4.9 times higher than that of 93AS3510. A concentration of 1 mg L-1 a.i. (active ingredient) of imazethapyr herbicide produced three distinctive response types in 93AS3510 crosses, while a concentration of 10 mg L-1 was required to differentiate the three response types in PWC-16 crosses. The segregation of the herbicide-resistant gene from both Clearfield rice lines fit into the Mendelian 1:2:1 (susceptible:intermediate:resistant) ratio. There was no maternal effect associated with the inheritance of the trait. The imidazolinone resistance, therefore, is governed by a single incomplete dominant nuclear gene. The F1 hybri...
Field Crops Research, 2010
ABSTRACT Red rice is the main weed in rice paddy fields. Imidazolinone herbicides in resistant rice cultivars currently provide a unique opportunity to control red rice in large-scale rice fields. However, the continuous use of this technology has resulted in imidazolinone-resistant red rice biotypes. This study aimed to identify the mechanism of herbicide resistance and the frequency and spatial distribution of the known imidazolinone herbicide-resistant alleles in red rice. The nucleotide sequence of the ALS gene indicated that the G654E, S653D and A122T mutations are present in the imidazolinone herbicide-resistant rice cultivars IRGA 422 CL, SATOR CL and PUITÁ INTA CL, respectively. This information and the nucleotide sequence surrounding these mutations were used for the development of single nucleotide polymorphism (SNP) molecular markers to identify the possible mutations that confer herbicide resistance in red rice. This analysis was carried out in a total of 481 plants from 38 populations collected as individuals that escaped control with the herbicides imazethapyr and imazapic in rice paddy fields in Southern Brazil. The G654E mutation was the most frequent, being found in 100% and 90.9% of the populations in the 2006/2007 and 20007/2008 seasons, respectively. In addition, the S653D and A122T mutations were also present either alone or as double or triple mutations in some plants. Target site insensitivity is the predominant mechanism of resistance in red rice resistant to imidazolinone herbicides in Southern Brazil. The high frequency of the S653D mutation, the same mutation responsible for the resistance in the rice cultivar largely used in Southern Brazil, indicates that gene flow is occurring from the rice cultivar to red rice. Management practices related to increasing crop sanitation and decreasing of herbicide selection pressure through crop rotation should be enforced to prevent the evolution of herbicide resistance in red rice.
2021
Increasing agricultural productivity is indispensable to meet future food demand. Crop im-provement programs rely heavily on genetic diversity. The success of weeds in the ecosystem can be attributed to genetic diversity and plasticity. Weedy rice, a major weed of rice, has diverse morphology and phenology, implying wide genetic diversity. Study was conducted to genotype weedy rice accessions (n =54) previously phenotyped for herbicide tolerance and allelopathic potential using 30 SSR markers. Cultivated rice (CL163, REX) and allelopathic rice (RONDO, PI312777, PI338047) were also included in the study. Nei’s genetic diversity among weedy rice (0.45) was found to be higher than cultivated rice (0.24) but less than allelopathic rice (0.56). The genetic relationship and population structure based on herbicide tolerance and allelopathic po-tential were evaluated. Herbicide-tolerant and susceptible accessions formed distinct clusters in the dendrogram, indicating their genetic variation...
Development of herbicide-tolerant irrigated rice cultivars
Pesquisa …, 2010
The objective of this work was to develop new irrigated rice lines tolerant to imidazolinone herbicides. The backcross breeding procedure was used to transfer the imidazolinone tolerance allele from mutant 93AS3510 to the recurrent parents 'BRS 7 Taim' and 'BRS Pelota'. Individual herbicide-tolerant plants were selected in each generation, for three backcrossings (RC 1 to RC 3), followed by three selfing generations (RC 3 F 1 to RC 3 F 3). The best four RC 3 F 3 lines for agronomic traits were genotyped with 44 microsatellite markers. The observed conversion index of the new imidazolinone-tolerant lines varied from 91.86 to 97.67%. Pairwise genetic distance analysis between these lines and 22 accessions from the Embrapa's Rice Germplasm Bank clustered the new lines with their respective recurrent parents, but not with 'IRGA 417', which was originally used as recurrent parent to derive IRGA 422 CL, the only imidazolinone-tolerant irrigated rice cultivar recommended for cultivation in Brazil. Therefore, these lines represent new options of genetically diverse imidazolinone-tolerant rice accessions. Lines CNA10756 ('BRS Sinuelo CL') and CNA10757 will be released for cultivation in the Clearfield irrigated rice production system in Rio Grande do Sul, Brazil.
Euphytica, 2012
Several red rice biotypes have evolved resistance to imidazolinone herbicides. The origin of resistance has been attributed to gene flow from the herbicide-resistant rice cultivars, but independent evolution of spontaneous mutation can also contribute to the herbicide resistance. The objective of this study was to quantify the occurrence of gene flow and independent selection as the mechanisms of origin of imidazolinone-resistance in red rice to correctly define the management practices for red rice control. Three single nucleotide polymorphism markers were used to identify acetolactate synthase (ALS) gene mutations, and four simple sequence repeat markers were used to identify hybrids of imidazolinone-resistant rice cultivars and red rice. In addition, genetic diversity and population structure analyses were performed. Artificial hybrids were used as controls. Gene flow was the main origin of imidazolinone herbicide resistance, but independent selection occurred in 1.1 % of the evaluated red rice plants. Two red rice plants that independently evolved herbicide resistance had the ALS gene mutation, Gly 654 Glu. Population structure analysis also indicated intense gene flow from rice cultivars to red rice, but some populations maintained a high genetic identity based on a small amount of gene introgression from the rice cultivars. These results indicate the importance of adopting controls of red rice escapees to avoid gene flow from the imidazolinone-resistant rice, and the necessity of biotechnological approaches to mitigating gene flow in the development of new herbicide-resistant rice cultivars.
Developing dual herbicide tolerant transgenic rice plants for sustainable weed management
Scientific reports, 2018
Herbicides are important constituents of modern integrated weed management system. However, the continuous use of a single herbicide leads to the frequent evolution of resistant weeds which further challenges their management. To overcome this situation, alternating use of multiple herbicides along with conventional weed-management practices is suitable and recommended. The development of multiple herbicide-tolerant crops is still in its infancy, and only a few crops with herbicide tolerance traits have been reported and commercialized. In this study, we developed transgenic rice plants that were tolerant to both bensulfuron methyl (BM) and glufosinate herbicides. The herbicide tolerant mutant variant of rice AHAS (Acetohydroxyacid synthase) was overexpressed along with codon optimized bacterial bar gene. The developed transgenic lines showed significant tolerance to both herbicides at various stages of plant development. The selected transgenic lines displayed an increased toleranc...
The IMI-herbicides rice package has been recognized by all means among the most efficient chemical approaches for weedy rice control nowadays. Inevitably, the continuous and sole dependence, as well as ignorance on the appropriate use of imidazolinone herbicides in the IMI-herbicides rice package by rice growers has caused the development of herbicide resistance in weedy rice populations across many IMI-herbicides rice package adopted countries, inclusive of Malaysia. Hence, a comprehensive study was conducted to elucidate the occurrence, level, and mechanisms endowing resistance to IMI-herbicides on field-reported resistant (R) weedy rice populations collected from IMI-rice fields in Kampung Simpang Sanglang, Perlis (A), Kampung Behor Mentalon, Perlis (B), and Kampung Sungai Kering, Kedah (C). The collected weedy rice populations were compared with a susceptible weedy rice population (S), an imidazolinone-resistant rice cultivar (IMI-rice), and a susceptible local rice cultivar (MR...
Pesticide Biochemistry and Physiology, 2013
A putative resistant red rice (Oryza sativa) accession, an imidazolinone-resistant rice cultivar (Clearfield), a susceptible red rice accession and a susceptible rice cultivar were evaluated for cross-resistance to imazamox and imazethapyr in a whole-plant response experiment and seed bioassay. Additionally, a 210-bp fragment of the ALS gene was sequenced to identify mutations responsible for resistance. Also, a 574 bp of the ALS gene was sequenced and PCR for detection of the 'Clearfield allele' was conducted by the Andalusian Institute of Agricultural Research and Training (IFAPA, Spain). In the whole-plant response experiment, the putative resistant red rice was >23 and >21 times more resistant to imazamox and imazethapyr than the susceptible accession, respectively, whereas the respective resistance factor values based on seed bioassay were 86.4 and 141.7. Also, the respective resistance factor values for the Clearfield rice cultivar were similar with those calculated for putative resistant red rice. Additionally, the sequence of the 210 bp ALS gene fragment from the putative resistant red rice and Clearfield rice cultivar revealed the same amino acid substitution of Ser653Asn in both alleles (homozygous). Furthermore, the sequence of a 574 bp ALS gene fragment and the PCR for detection of the 'Clearfield allele' confirmed that the putative resistant red rice is homozygous mutant for the Ser653Asn mutation and provided additional evidence that its genetic background matches that of Clearfield rice. These findings indicate clearly that the insufficient control of the putative resistant red rice with imazamox was due to target-site resistance and particularly due to a point mutation at the Ser653 codon, which is positively identified as having been derived from the Clearfield rice cultivar.