Molecular mapping in oil radish ( Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode ( Heterodera schachtii (original) (raw)
2009, Theoretical and Applied Genetics
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible). A dominant major QTL Hs1Rph explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1Rph from radish into rapeseed by means of chromosome addition lines.
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Theoretical and Applied Genetics, 2003
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This book was prepared to describe the scientific principles of the construction of genetic maps using an intera-specific F2 population and two PCR-based markers RAPDs and AFLPs. The book also describes the utilization of the construction of genetic maps in plant breeding programs, including gene(s)/QTL(s) mapping of desired traits, gene(s) isolation and gene(s) transfer. The mapped gene(s) can be used successfully in marker assisted selection (MAS) programs. This book will be a value added scientific resource for the MSc and PhD students who are interested to do their thesis’s and dissertations in the field of plant genome, gen(s) and QTL(s) mapping. Simultaneously, the presented work well be a helpful lab manual for the young scientists and Post-Doc researchers due to the powerful information, protocols’, techniques and detailed statistical methodology were included in the book chapters.
An extended map of the sugar beet genome containing RFLP and RAPD loci
Theoretical and Applied Genetics, 1995
An updated map of sugar beet (Beta vulgaris L. ssp. vulgaris var ‘altissima Doell’) is presented. In this genetic map we have combined 248 RFLP and 50 RAPD loci. Including the loci for rhizomania resistance Rr1, hypocotyl colour R and the locus controlling the monogerm character M, 301 loci have now been mapped to the nine linkage groups covering 815 cM. In addition, the karyotype of some of the Beta vulgaris chromosomes has been correlated with existing RFLP and RAPD linkage maps.
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