Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection (original) (raw)
Related papers
Enhanced bactericidal effect of silver nanoparticles synthesized using marine brown macro algae
In the present investigation silver nanoparticles of spherical shape was synthesized biologically by rapid biological drip method using marine brown macro algae, Sargassum wightii (S. Wightii). Seaweed extract act as a reducing agent for silver nitrate. Silver nanoparticles have been characterized by UV-Visible spectrophotometer, Fourier-Transform Infrared spectroscopy (FTIR), X-ray diffractometer (XRD) and High Resolution Transmission Electron Microscope (HR-TEM). Antibacterial activity of these nanoparticles was carried out by antibiotic disc diffusion method against pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Seaweed S. wightii mediated silver nanoparticles were found to be effective against these pathogens than silver nitrate.
SEAWEED SILVER NANOPARTICLES: CHARACTERIZATION, GREEN SYNTHESIS, AND BIOACTIVITY ANALYSIS
Deuton-X Ltd., 2023
An important area of nanotechnology is the development of experimental procedures for the synthesis of nanoparticles that are biologically inspired. New environmentally friendly "green" synthesis techniques are being developed to address the rising demand for commercial nanoparticles. In this study, Ulva fasciata sea weed extract was used to synthesize stable silver nanoparticles (AgNPs). The synthesis of AgNPs and the reduction of Ag ions in the media are both observed using a UV-Vis spectrometer. AgNPs that have been synthesized have had their morphology studied using XRD and SEM. The peaks in the XRD pattern correspond to the metallic silver's face-centered-cubic (FCC) structure. Silver nanoparticles are discovered to have an average grain size of 6.35 nm. TGA/DTA results showed that desorption of chemisorbed water was associated with exothermic reaction and weight loss. The carbonyl, hydroxyl, amine, and protein functional groups that form a layer atop AgNPs and stabilize them in a medium were identified using FTIR. Moreover, the antibacterial activity of silver nanoparticles was done and the results were phenomenal with large inhibition zones.
Nano Biomedicine and Engineering, 2016
Utilization of renewable marine resources for the biosynthesis of metal nanoparticles is an innovative area in the current nanotechnology research. The present article reports a simple environmentally benign method for the synthesis of silver nanoparticles using aqueous extract of marine brown seaweed Padina Tetrastromatica, which act as both the reducing and stabilising agent. The formation, morphology and crystalline nature of the synthesised silver nanoparticles were determined using UV-Visible Spectroscopy, Transmission electron microscopy, Selected area electron diffraction and X-ray diffraction techniques. The surface plasmon resonance peak at 424 nm in the UV-Visible spectrum confirmed the formation of silver nanoparticles. The biosynthesised silver nanoparticles were almost spherical with an average size of 15 nm. Crystalline nature of silver nanoparticles is evident from the characteristic peaks in the X-ray diffraction pattern, bright circular spots in the selected area electron diffraction pattern and clear lattice fringes in the High resolution Transmission electron microscopy images. Fourier transform infrared spectroscopic analysis revealed the presence of functional bio active metabolites in seaweed extract which is responsible for the formation and efficient stabilization of the nanoparticles. The biosynthesised silver nanoparticles show excellent antimicrobial activity against Escherichia coli and Aeromonas hydrophila. They also show very good catalytic activity for the reduction of methylene blue in the presence of NaBH4 in aqueous medium. Thus the green synthesised nanoparticles are expected to be a promising candidate for the application in diverse fields.
Antibacterial properties of biosynthesized silver nano particles
International Journal of Pharmacometrics and Integrated Biosciences, 2020
Nanoparticles have their demand in various fields of science and technology and their applications extend even in medical and pharmaceutical arena. They have been used as preservatives, diagnosing aids and potent antibacterial agents. But their production is a serious matter of concern when it comes to cost, efficacy and toxicity issues. Overcoming these limitations green synthesis has taken its advantage for their commercial and large scale synthesis. This research will focus on the preparation of nano particles of silver with the help of purified leaf extract from Lannea coromandelica and evaluation of the same using UV-Vis Spectrophotometry. The nanoparticles exhibited surface plasmon resonance at 420nm in UV spectroscopy. Futhermore, nanoparticles have been evaluated for their antibacterial activity on Putida vulgaris, Staphylococcus aureus, and Bacillus subtillis. The results proved the eco friendly synthesized silver nanoparticles have a good antibacterial and can be used effe...
Journal of drug delivery, 2018
Antimicrobial study of biofunctionalized silver nanoparticles has been done with the emphasis on its mechanism on both gram positive and negative bacteria. The biofunctionalized silver nanoparticles are employed considering their importance in green chemistry with respect to easy synthesis, usefulness, and economic synthetic procedure involved. The stability of these nanoparticles was determined by zeta potential analyzer. The probable mechanism of antibacterial activity was performed on Proteus mirabilis by field emission scanning electron microscopy (FESEM) and energy dispersive spectroscopy (EDAX) study which does not show the presence of silver. The free radicals generated by silver nanoparticles were responsible for lethal antibacterial activity by rupturing the cell surface which causes improper nutrient and signal supply. Free radical scavenging efficacy of silver nanoparticles was confirmed by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method. AgNP enhanced the membrane leakage of reducing sugars by destroying the proteins existing on the cell wall. These nanoparticles are found to be toxic against human pathogens and are highly effective on Staphylococcus aureus. The effect of silver nanoparticles is concentration dependent and independent of the type of strains used.
Green Synthesis of Silver nanoparticles Using Marine Algae Extract and Their Antibacterial Activity
2018
In this study, we used an environmentally friendly chemistry to synthesize nanoparticles (AgNPs) embedded biological marine extracts (BME) from four species of marine algae. Twelve extracts from four species of seaweeds (U. fasciata , Grateloupia sp., P. capillacea and C. mediterranea) were used for synthesis AgNPs/BME, antibacterial properties were investigated and hence anti-biofilm activity against indicator strains and bacterial community using well-cut diffusion technique and pouring technique. The positive records of antibacterial activity ranged between 2.4 and 23.6 AU. Occasionally, as well as, SUDW showed high AU (6.6) against bacterial community in water from both harbors. Aqueous extract of Ulva fasciata was selected highly efficient, among all algae species screened for the green synthesis of AgNPs/BME. The antibacterial activity of AgNPs/UE against both gram-positive and gram-negative bacteria(Staphylococcus aureus, Streptococcus faecalis, Pseudomonas aureogenosa, Esche...
Biological or green synthesis of nanoparticles is a relatively new emerging field of nanotechnology which are cheaper, less-labor and eco-friendly benefits over chemical and physical processes of synthesis. In the present work, three different seaweeds species (Caulerpa prolifera, Sargassum acinarium and Gigartinia teedi) aqueous extracts were compared as bioreductants for the synthesis of nanostructure silver particles (Ag-NPs). Structural; morphological and optical properties of the synthesized nanoparticles have been characterized systematically by using ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). UV–visible spectrometry study revealed different surface plasmon resonance according to species of seaweeds and incubation time. The nanoparticles were crystalline in nature confirmed by the XRD pattern. A TEM images showed that the particles are spherical in shape with size ranging from 2 to 22nm according to the species. FT-IR spectroscopy confirmed the presence of bio-components in the seaweeds extracts which were responsible for the nanoparticles synthesis. The synthesized nanoparticles have highly effective antimicrobial activity against eight tested pathogenic microbial strains compared to their corresponding seaweed extracts, silver nitrate and ampicillin as controls. Results of the present study recommended green synthesis of Ag-NPs from seaweeds to play a vital role in future for industrial and/or therapeutic applications.