EFFECT OF IPOMOEA PES-CAPRAE (LINN.) R. BR. STEMAND ROOT EXTRACTS ON DIFFERENT CLINICAL BACTERIAL ISOLATES. (original) (raw)
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In vitro studies of antibacterial and antioxidant activity of Ipomoea pes-caprae root extracts
To evaluate the antibacterial and antioxidant activity of Ipomoea pes-caprae root (belonging to the family of Convolvulaceae) was studied in five different solvents. Antibacterial activity was determined using well diffusion assay for eight strains of bacteria. Minimum inhibitory concentrations were determined in eight bacterial strains by agar well diffusion assay. Other focuses included the determination of antioxidant activity using DPPH assay and IC50 (Inhibitory concentration) values were also determined using broth dilution assay. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, tannins, saponins and phenolics. The presence of these bioactive constituents is associated with the antibacterial activity of the plant. Methanol extracts of I.pes-caprae exhibited highest inhibition zone of 17mm, 18mm and 21mm against Klebsiella pnemoniae, Escherichia coli, and Bacillus subtills. During DPPH assay of methanol extract of I.pes-caprae root shows highest antioxidant activity of 92.04% in 200(µg/ml). The results confirm that I.pes-capra roots can be used as source of drugs to fight infections caused by susceptible bacteria.
Antimicrobial Activity of Ipomoea pes-caprae L. against Selected Microbial Species
International Journal of Scientific Research in Science and Technology, 2019
In the present investigation, petroleum ether, chloroform, ethyl acetate and methanol extracts of leaves of Ipomea pes-caprae were studied for the antibacterial and antifungal activities against Gram-positive bacteria such as Bacillus pumilus, B. subtilis, Micrococcus luteus and Staphylococcus aureus, Gram-negative bacteria, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia fungal strains such as Aspergillus niger, A. fumigates and A. flavus and the results are presented. The mean zone of inhibition for bacteria ranged from 8.5 to 25.6 mm. The minimum inhibitory concentration and minimum bactericidal concentrations were from 15.62 to 500 µg/mL and 31.25 to 1000 µg/mL respectively. The standard drug, Ciprofloxacin (10 µg/disc) was used for standard bacterial strains and recorded mean zone of inhibition ranged from 23.5 to 29.5 mm. The mean zone of inhibition for Aspergillus species ranged from 9.6 to 21.5 mm. The minimum inhibitory concentration and minimum fungicidal concentrations were from 31.25 to 500 µg /mL and 62.5 to 1000 µg/mL respectively. The standard drug, Ketoconazole (5 µg/disc) was used for Aspergillus species and recorded mean zone of inhibition ranged from 23.5 mm to 26.5 mm.
Journal of Pharmacy and Alternative Medicine, 2014
pomoea nil (Linn) Roth, (morning glory / kaladanah; Convolvulaceae), is frequently grown in gardens and lawns for its ornamental flowers and often runs wild in hedges and wastelands. Plant is documented to possess beneficial effect in erectile dysfunction, impotence and also have antispasmodic, bronchodilator, blood purifier, diuretics, astringent, anti-inflammatory and hepato-protective etc. In this study, disc diffusion method was adopted to evaluate and compare the antimicrobial activity of the aqueous and organic (DCM, ethanol, methanol) extracts of the seeds and whole plant (leaves, flower and bark) of Ipomoea nil, in order to know the best extract and plant part having the beneficial activity against pathogenic bacteria species of both G +ve strains, i.e., Staphylococcus aureus, Bacillus pumilus, Streptococcus pneumoniae, G -ve strains, i.e., Escherichia coli, Citrobacter freundii and Klebsiella pneumoniae and two species of fungi (Candida albicans, Aspergillus niger). Methanolic and ethanolic extract of the seeds of the Ipomoea nil showed significant antibacterial activity against Staphylococcus aureus (G +ve) having the relative percentage of inhibition of 89.88 % and 85.67 % respectively, while methanolic extract of the whole plant also have relative percentage of inhibition of 83.96%, whereas ethanolic extract of the whole plant have good antibacterial activity. Aqueous and DCM extract of seeds and whole plant showed weak inhibitory response. Modified agar well diffusion method was adopted to measure the minimum inhibitory concentration (MIC) and MIC values for G +ve, lies within the range of 30 to 60 μg /ml (seeds), 60 to 90 μg /ml (whole plant) and for the Gram -ve, it lies within the range of 90 to 250 μg /ml (seeds), 180 to 500 μg /ml (whole plant) and for the fungi, it varies from 500 to 2000 μg /ml. Present study clearly indicate that the antimicrobial activity varies from part to part and the plant material used and it also indicate that the methanolic extract of Ipomoea nil is a potentially good candidate for the therapy of antibacterial-resistant bacteria and would therefore require further study.
Phytochemical and Antibacterial Properties of Leaf Extracts of Ipomoea asarifolia
Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.
Antibacterial and Cytotoxic Activities of Extracts and Isolated Compounds of Ipomoea turpethum
Pak. J. Biological Sci.,, 2002
D glucoside), H-2 (22, 23-dihydro-"-spinosteryl glucoside) & CH-2 (salicylic acid) isolated from the chloroform extract of stem of Ipomoea turpethum and the crude petroleum ether, chloroform and ethyl acetate extracts were screened against thirteen pathogenic bacteria for their antibacterial activities. The minimum inhibitory concentration (MIC) of the isolated compound CH-2 was also measured against Bacillus subtilis, Shigella dysenteriae, Sarcina lutea and Escherichia coli. The values were found to be between 128 and 256 µg/ml. The cytotoxic activity of CH-2 and chloroform and ethyl acetate extracts was also measured by brine shrimp lethality bioassay and the LC values were found 50
ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF IPOMOEA HEDERACEA
Farmacia
Abstract Research on antimicrobial and anti-oxidant activity from plant sources is increasing due to positive effects on human health. However relatively little work has been done to investigate the antimicrobial and antioxidant effects of methanolic extracts from Ipomoea hederacea. In the present study methanolic extracts of leaves, flowers, stem, seed and roots from Ipomoea hederacea were evaluated using an antimicrobial assay, the determination of total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and the total antioxidant capacity. Our results suggested that methanolic extracts exhibited high antimicrobial effects when tested against various bacterial and fungal strains (i.e. B. subtilis, P. multocida, S. aureus, E. coli, A. niger, A. flavus, A. alternate and R. solani). The total phenolic content showed the highest value for stem extract (131.11±1.22 mg/g), and the lowest for root extract (74.44±1.52 mg/g). The tested extracts were able to reduce the stab...
Extracts from the seeds of two morning glories were studied to determine their anti-microbial activity. Ipomoea alba and Ipomoea tricolor were analyzed by broth microdilution technique. I. alba extract showed very strong antibacterial activity against Enterococcus faecalis and Bacillus cereus, with minimum inhibitory concentration (MIC) values of 19.5 µg/mL for both. Antifungal activity was also observed against Cryptococcus neoformans, with MIC of 78 µg/mL. Strong cytotoxicity against the human breast cancer cell line MDA-MB-231, with IC50 value of 35.72 ± 1.86 µg/mL was observed. I. tricolor extract showed strong activity against E. faecalis and B. cereus, with MIC values of 19.5 and 9.75 µg/mL, respectively. Strong activity was observed against Cryptococcus neoformans, with MIC of 9.75 µg/mL. Strong cytotoxicity was also observed against human breast cancer cell lines MDA-MB-231 and Hs 578T, with IC50 values of 28.47 ±1.98 µg/mL and <30 µg/mL, respectively.
Pharmacognosy …, 2011
Background: Ipomoea batatas (L.) Lam., popularly known as sweet potato (SP), has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. Ethnopharmacological data show that SP leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in Brazil. The aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves’ extract of SP leaves.Materials and Methods:The screening was performed for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. The color intensity or the precipitate formation was used as analytical responses to these tests. The total antioxidant capacity was evaluated by the phosphomolybdenum complex method. Antimicrobial activity was made by agar disk and agar well diffusion tests.Results:The phytochemical screening showed positive results for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. Total contents of 345.65, 328.44, and 662.02 mg were respectively obtained for alkaloids, anthraquinones, and phenolic compounds in 100 g of the dry sample. The total antioxidant capacity was 42.94% as compared to ascorbic acid. For antimicrobial studies, no concentration of the SP freeze dried extract was able to inhibit the growth of Streptococcus mutans, S. mitis, Staphylococcus aureus, and Candida albicans in both agar disk and agar well diffusion tests.Conclusions:SP leaves demonstrated the presence of secondary metabolites with potential biological activities. No antimicrobial activity was observed.
An In-Vitro Assessment of Antimicrobial, Thrombolytic and Cytotoxic Activity on Ipomoea Pes-Tigridis
2014
To explore activities antimicrobial, thrombolytic, cytotoxic of the ethyl acetate extract and n-hexane extract of Ipomoea pes-tigridis leaves. Antimicrobial study was measured by noticing zone of inhibition. In vitro thrombolytic activity by clot lysis method and cytotoxic activity was considered by using brine shrimp lethality bioassay.Anti-microbiological screening ethyl acetate extract of leaves showed mild antimicrobial activity with zone of inhibition ranging from 11 to 14 mm and n hexane also showed mild antimicrobial activity with zone of inhibition ranging from 4 to 9 mm as compared to standard ciprofloxacin (zone of inhibition of 50 mm). In vitro thrombolytic model was applied to find out the clot lysis effect of ethyl acetate and n-hexane extracts of Ipomoea pes-tigridis where streptokinase and water were employed as a positive and negative controls, respectively. The extracts showed thrombolytic activity 14.90% and 8.18%. In Brine Shrimp Lethality Bioassay, ethyl acetate ...