Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana (original) (raw)

1999, Journal of Chromatography A

A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2′-deacetoxydecinnamoyltaxinine J and 2′-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV–Vis as well as photodiode array detection.