Isolation and Identification of Diesel oil-degrading Bacteria in used engine oil contaminated soil (original) (raw)
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The present work was conducted to isolate and identify bacteria from oil- 21 contaminated soil to evaluate their role in biodegradation of commercial diesel under laboratory 22 conditions. Diesel fuels are used by different vehicles, diesel generators and especially heavy 23 transport vehicles. Its manufacturing, transportation, utilization and disposal have the threat to 24 pollute the surrounding environment. Biodegradation is one of the biological processes to 25 remediate the pollutants. This is the cheaper and easy method as compared to other methods like 26 direct burning, land foaming and bioventing because diesel smoke has a carcinogenic effect. 27 In the current exploration ordinary bacterial species that degrade diesel were isolated from 28 selected areas of Mansehra. Six sites were selected and a total of 60 samples were collected. 29 These sites were coded according to their names. For the isolation of diesel degrading bacteria 30 soil samples were analyzed. The isolation of bacteria was done on nutrient agar, nutrient broth 31 and mineral salt medium (MSM), and IR spectroscopy was used to observe the degradation of 32 diesel. Twenty samples were found having bacterial growth. Further analysis showed that four 33 samples BHG, SHNK, TAK and KMOR had diesel degrading capability as these showed 34 positive results. The isolated bacteria were identified by morphological and biochemical features, 35 while the degradation of diesel was determined qualitatively by interpreting the intensity of the 36 peaks of IR spectroscopy. The intensity of the peaks in the spectrum of control was compared 37 with the intensity of the peaks in the spectrum of the test samples which indicated degradation of 38 diesel. Three bacterial species, namely, Bacillus cereus, Bacillus subtilis and Pseudomonas 39 aeruginosa were identified using Berge's manual method. The bacterial species were tested for 40 their capability to degrade commercial diesel presented at different concentrations i.e. 1000 ppm 41 and 5000 ppm.
2014
Abstract: The biodegradation potential of five bacteria isolated from used engine oil contaminated soil was investigated in this study. The bacteria were inoculated into used oil supplemented with Mineral Salt Medium and incubated at 37 o C for 25 days. The results obtained showed that all the bacteria were able to utilize the engine oil as source of carbon. IR analysis of the residual engine oil showed that the bacteria have exerted microbial action on the oil. The results obtained from IR analysis revealed 9 bands in P. vulgaris, B. cereus, S. typhi, S. faecium, 10 bands for P. aeruginosa while that of the control was recorded to 8 bands. This study showed that the bacteria strains used in this study could be relevant in the bioremediation of ecosystem that may be contaminated with hydrocarbons
Isolation and Characterization of Diesel Degrading Bacteria from Petroleum Oil Contaminated Soil
Bacterial Empire
Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).
Journal of Biochemistry, Microbiology and Biotechnology
The high rise in the exploration and usage of petroleum products have led to widespread contamination in the environment. Thence, the continuous search for microorganisms with the potential to mineralize these pollutants is necessary. This study was conducted to isolate, identify and determine the diesel degradation potential of bacteria from oil-contaminated soil collected from three filling stations in Azare, Katagum LGA, Bauchi State, Nigeria. The diesel degrading bacteria were identified using standard protocols. The isolates were screened spectrophotometrically for their potential to utilize 1% diesel (v/v) as their sole carbon and energy source and the best candidate was used for determining the effect of diesel concentration on its biodegradation. The results revealed the presence of three bacterial species including Pseudomonas aeruginosa, Pseudomonas putida and Bacillus subtilis. P. Putida showed the highest diesel degradation at 120 h with an absorbance of 2.27±0.03 at 600...
The continuous and indiscriminate discharge of crude and/or refined oils during the various processing and transportation of oils leads to the contamination of the environment. This study was aimed at determining the bacterial diversity of contaminated soils around tank farm areas and the changes that occur in the oil hydrocarbon components during the process of biodegradation by the selected bacterial species. Contaminated soil samples were collected around different tank farm areas based on their petroleum contents at the Nigerian National Petroleum Corporation (NNPC) depot, Apata, Ibadan, Oyo State, Nigeria. The total heterotrophic bacterial count ranged from 1.04 × 10 7 to 2.73 × 10 7 cfu/ml in the various contaminated soil samples. The hydrocarbon utilizing bacteria count ranged from 1.9 × 10 3 to 7.3 × 10 3 cfu/ml in the contaminated soil samples. Among the 28 bacterial isolates capable of utilizing crude oil vapour, two isolates thrived on the highest crude oil concentration employed during screening and were used singly and in a mixed culture for the biodegradation of the contaminated soil samples. The two hydrocarbon-utilizing bacteria isolates were characterized and identified as Pseudomonas species (AG13) and Bacillus species (PM4). The percentage degradation of the aliphatic and aromatic hydrocarbons in the contaminated soil samples after 8 weeks was highest in the Pseudomonas species (AG13) treated sample with 71.51% and 63.30% respectively and this was followed closely by the mixture of both bacteria isolates with 67.14% and 50.69% respectively and Bacillus species (PM4) having the lowest with 31.92% and 19.03% respectively. Therefore, hydrocarbon-utilizing bacteria species can be employed in the removal of oil pollutants from the soil, but a careful selection is required to ensure effectiveness.
Isolation and characterisation of bacteria from petroleum oil contaminated soil
Petroleum contaminated soil samples were collected and investigated for the presence of bacteria that can biodegrade petroleum hydrocarbons. The samples were collected from a car repair garage from the surface and at 5 cm down the surface. Both samples showed growth on Nutrient agar (NA) and Nutrient Broth (NB) supplemented with Diesel and Gasoline oils as sole carbon source. However, the surface samples had more growth compared to the 5 cm sample. Isolated strain X1, X2 and X3, were found Gram’s negative. X1 was rod and X2 and X3 were coccobacilli type. API 20E and API 20NE identified Three potent bacteria strains capable of degrading hydrocarbons from the genera Mannheimia haemolytica (X1), Sphingomonas paucimobilis (X2) and Brevundimonas vesicularis (X3). M. haemolytica had the highest growth and shortest lag phase compared to S. paucimobilis and B. vesicularis.
Indiscriminate release of hydrocarbon squander such as, spent motor oil (SEO) in the environment is becoming worrisome due to its likely effect on the soil ecosystem. The study assessed the capacity of some bacterial isolates to utilize spent motor oil. A total of fifteen (15) bacteria isolate comprising 4 distinct species of Bacillus, 3 Pseudomonas, 3 Citrobacter species, 3 Monococcus species and 2 Acinetobacter species were isolated. The ability of the pure culture and mixed bacterial consortium to degrade spent motor oil was tested in a shake-flask culture containing mineral salt medium (MSM) supplemented with 2% (V/v) spent motor oil and 5% (V/v) bacteria isolate and the residual hydrocarbon content was measured gravimetrically after 28days of incubation. The percentage utilization of the pure isolates were 78.20%, 74.82%, 56.28%, 61.28%and 50.66% after 28 days of incubation for Bacillus, Pseudomonas, Monococcus, Acinetobacter and Citrobacter species respectively while the consortium of bacteria degraded 89.27% of spent motor oil under similar conditions. Thus, the consortium of bacteria was more effective in the degradation of SMO in soil. Based on our findings, we recommend the use of bacterial isolates for remediation of spent oil contaminated soil since it is eco