PCR Detection of Indicator Genes in Methicillin-Resistant Staphylococcus aureus (mrsa) isolated from Three Saudi Hospitals (original) (raw)

The aim of the present study is to examine the recovered strains of methicillinresistant Staphylococcus aureus (MRSA) phenotypically by conventional identification. Genotypicalexamination was made also by polymerase chain reaction (PCR) to detect the genes; lukF encoding Panton-Valentine Leukocidin(PVL) and arcA an indicator of the arginine catabolic mobile element (ACME).Twinty-eight strains of Staphylococcus aureus, collected in 2013 from three Saudi central hospitals, were characterized by streaking on Mannitol salt agar plates and biochemically identified as Staphylococcus aureus. Resistance towards eight antimicrobial agents revealed that most of the tested strains of Staphylococcus aureus showed resistance to the tested antimicrobials in the following order; Oxacillin 100%, Tetracycline 71%, Cefoxitin 71%, Erythromycin 71%, Ciprofloxacin 71%, Imipenem 68%, Amikacin 60% and Vancomycin 7 %. All the tested strains produced the gene arcA, while 80% showed the presence of lukF. Al-Talib, H. et al.., (2013): Rapid detection of methicillin-resistant Staphylococcus aureus by a newly developed dry reagent-based polymerase chain reaction assay, Journal of Microbiology, Immunology and Infection, http://dx.doi.org/10.1016/j.jmii.2013.06.004\. Baddour, M.; Abuelkheir, M. and Fatani, A. (2006): Trends in antibiotic susceptibility patterns and epidemiology of MRSA isolates from several hospitals in Riyadh, Saudi Arabia. Annals of Clinical Microbiology and Antimicrobials, 5: 30, 1-11. Bergan, T.; Bruun, J.N.; Digranes, A.; Lingaas, E.; Melby, K.K. and Sander, J. (1997): Susceptibility testing of bacteria and fungi. p. 251 illus.